Establishment of a laticifer-specific gene expression system in Hevea brasiliensis

• Project/Area Number: 22780298
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied molecular and cellular biology
• Research Institution: Tohoku University
• Principal Investigator: TAKAHASHI Seiji 東北大学, 大学院・工学研究科, 准教授 (90343061)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 発現制御 / 天然ゴム / パラゴムノキ / 遺伝子発現制御 / ラテックス / EST

Research Abstract

Natural rubber obtained from latex in Hevea brasiliensis has the excellent physical properties, especially required for tire manufacturing. To meet the continuously increasing demand for natural rubber, metabolic engineering of H. brasiliensis is required to improve natural rubber production, achieved by a latex-specific overexpression of genes involved in the natural rubber biosynthesis. In this study, we have succeeded in discovering of latex-specific genes and cloning of 5'-upstream regions of these latex specific genes. Furthermore, we also identified several latex-specific transcription factors, expected to participate in the regulation of the latex-specific gene expression.

Research Products

• [Journal Article] Transcription analyses of GmICHG, a gene coding for a b-glucosidase that catalyzes the specific hydrolysis of isoflavone conjugates in Glycine max (L.) Merr (2013)
  • Author(s): DongChan Yoo
  • Journal Title: Plant Science Volume: 208 Pages: 10-19
  • DOI: 10.1016/j.plantsci.2013.03.006
  • Peer Reviewed
• [Journal Article] Purification, gene cloning, and biochemical characterization of a β-glucosidase capable of hydrolyzing sesaminol triglucoside from Paenibacillus sp. KB0549 (2013)
  • Author(s): Nair, A., Kuwahara, A. Nagase, A. Yamaguchi, H. Yamazaki, T., Hosoya, M., Omura, A., Kiyomoto, K., Yamaguchi, M., Shimoyama, T., Takahashi, S., and Nakayama, T.
  • Journal Title: PLOS ONE Volume: 8 Issue: 4 Pages: e60538-e60538
  • DOI: 10.1371/journal.pone.0060538
  • Peer Reviewed
• [Journal Article] Characterization of <i>cis</i>-prenyltransferases from the rubber producing plant <i>Hevea brasiliensis</i> heterologously expressed in yeast and plant cells (2012)
  • Author(s): Seiji Takahashi, Hye-Jin Lee, Satoshi Yamashita, Tanetoshi Koyama
  • Journal Title: Plant Biotechnology Volume: 29 Issue: 4 Pages: 411-417
  • DOI: 10.5511/plantbiotechnology.12.0625a
  • NAID: 10031122168
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] Identification and characterization of a cis,trans-mixed heptaprenyl diphosphate synthase from Arabidopsis thaliana (2012)
  • Author(s): Kota Kera, Seiji Takahashi, Tsuyoshi Sutoh, Tanetoshi Koyama and Toru Nakayama
  • Journal Title: FEBS Journal Volume: 279 Issue: 20 Pages: 3813-3827
  • DOI: 10.1111/j.1742-4658.2012.08742.x
  • Peer Reviewed
• [Journal Article] メタボロン・・・植物二次代謝工学におけるインパクト (2012)
  • Author(s): 中山亨、兪東燦、高橋征司
  • Journal Title: 生物工学会誌 Volume: 90 Pages: 576-581
  • NAID: 110009517335
• [Journal Article] Catalytic removal of acetaldehyde in saliva by a Gluconobacter strain (2012)
  • Author(s): Haruhiko Yamaguchi
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 114 Issue: 3 Pages: 268-274
  • DOI: 10.1016/j.jbiosc.2012.04.008
  • Peer Reviewed
• [Journal Article] メタボロン・・・植物二次代謝工学におけるインパクト (2012)
  • Author(s): 中山 亨
  • Journal Title: 生物工学 Volume: 90 Pages: 576-581
• [Journal Article] 包括的転写制御による植物イソプレノイドの代謝工学 (2011)
  • Author(s): 高橋征司、古山種俊、中山亨
  • Journal Title: 生物工学 Volume: 89 Pages: 649-652
  • NAID: 110008762374
• [Journal Article] Gene cloning and biochemical characterization of a catalase from Gluconobacter oxydans (2011)
  • Author(s): Yamaguchi, Haruhiko
  • Journal Title: JOURNAL OF BIOSCIENCE AND BIOENGINEERING Volume: 111 Issue: 5 Pages: 522-527
  • DOI: 10.1016/j.jbiosc.2010.12.021
  • Peer Reviewed
• [Journal Article] Functional Differentiation of the Glycosyltransferases That Contribute to the Chemical Diversity of Bioactive Flavonol Glycosides in Grapevines (Vitis vinifera) (2010)
  • Author(s): Eiichiro Ono, Yu Homma, Manabu Horikawa, Satoshi Kunikane-Doi, Haruna Imai, Seiji Takahashi, Yosuke Kawai, Masaji Ishiguro, Yuko Fukui and Toru Nakayama
  • Journal Title: Plant Cell Volume: 22 Issue: 8 Pages: 2856-2871
  • DOI: 10.1105/tpc.110.074625
  • Peer Reviewed
• [Journal Article] In vitro synthesis of high molecular weight rubber by Hevea small rubber particles (2010)
  • Author(s): Porntip Rojruthai, Jitladda Tangpakdee Sakdapipanich, Seiji Takahashi, Lee Hyegin, Motoyoshi Noike, Tanetoshi Koyama and Yasuyuki Tanaka
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 109 Issue: 2 Pages: 107-114
  • DOI: 10.1016/j.jbiosc.2009.08.009
  • NAID: 110007573238
  • Peer Reviewed
• [Journal Article] Production of tetraketide lactones by mutated Antirrhinum majus chalcone synthases (AmCHS1) (2010)
  • Author(s): Hatayama, M., Unno, H., Kusunoki, M., Takahashi, S., Nishino, T., and Nakayama, T
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 110 Issue: 2 Pages: 158-164
  • DOI: 10.1016/j.jbiosc.2010.02.010
  • NAID: 110007700563
  • Peer Reviewed
• [Journal Article] Production of tetraketide lactones by mutated Antirrhinum majus chalcone synthases (AmCHS1) (2010)
  • Author(s): Hatayama M, et al.
  • Journal Title: JOURNAL OF BIOSCIENCE AND BIOENGINEERING Volume: 110 Pages: 58-164
  • Peer Reviewed
• [Journal Article] Functional Differentiation of the Glycosyltransferases That Contribute to the Chemical Diversity of Bioactive Flavonol Glycosides in Grapevines (Vitis vinifera) (2010)
  • Author(s): Ono E, et al.
  • Journal Title: Plant cell Volume: 22 Pages: 2856-2871
  • Peer Reviewed
• [Presentation] Enzymatic Characterization of cis-Prenyltransferases from the Natural Rubber Producing Plant Hevea brasiliensis (2012)
  • Author(s): Seiji Takahashi
  • Organizer: 10th International Congress on Plant Molecular Biology
  • Place of Presentation: Jeju, Korea
• [Presentation] パラゴムノキ由来ラテックスに含まれる天然ゴム生合成酵素の機能解析 (2012)
  • Author(s): 高橋征司
  • Organizer: 第30回日本植物細胞分子生物学会大会・シンポジウム
  • Place of Presentation: 京都
• [Presentation] パラゴムノキ由来ラテックスに含まれる天然ゴム生合成酵素の機能解析 (2012)
  • Author(s): 高橋征司
  • Organizer: 第30回 日本植物細胞分子生物学会 大会・シンポジウム
  • Place of Presentation: 京都
• [Presentation] 高等植物のオーキシンを介した形態形成に対するシス型プレニルトランスフェラーゼの寄与 (2011)
  • Author(s): 高橋征司
  • Organizer: 第21回ドリコールおよびイソプレノイド研究会
  • Place of Presentation: 松江
• [Presentation] 葉緑体内イソプレノイド代謝制御におけるPhytochrome-interacting factorsの寄与 (2011)
  • Author(s): 高橋征司
  • Organizer: 第29回日本植物細胞分子生物学会大会
  • Place of Presentation: 福岡
• [Presentation] 葉緑体内イソプレノイド代謝制御における Phytochrome-interacting factorsの寄与 (2011)
  • Author(s): 高橋征司
  • Organizer: 第29回 日本植物細胞分子生物学会大会
  • Place of Presentation: 福岡
• [Presentation] 代謝経路を包括的に制御する転写因子による植物イソプレノイドの代謝工学 (2010)
  • Author(s): 高橋征司
  • Organizer: 第62回日本生物工学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-10-28
• [Book] 酵素タンパク質の一次構造に基づく遺伝子の単離同定(執筆担当部分第3章)、植物の分子育種学 (2011)
  • Author(s): 中山亨、高橋征司
  • Publisher: 講談社サイエンティフィック
• [Book] 植物の分子育種学 (2011)
  • Author(s): 鈴木正彦
  • Total Pages: 220
  • Publisher: 講談社サイエンティフィック
• [Remarks]
  • URL: http://www.che.tohoku.ac.jp/~seika/