Development of lipid-derived radical detection method

• Project/Area Number: 22790038
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Physical pharmacy
• Research Institution: Kyushu University
• Principal Investigator: YAMADA Kenichi 九州大学, 大学院・薬学研究院, 准教授 (60346806)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 脂質 / 過酸化脂質 / ラジカル / 蛍光 / スピン / 酸化ストレス / 脂質ラジカル / 磁気共鳴 / ニトロキシド

Research Abstract

Lipid-derived radicals and peroxides are involved in the pathogenesis of oxidative stress diseases. However, a useful lipid radical detection method has remained tentative. We focused on nitroxide reactivity, which allows spin trapping with carbon-centered radicals via radical-radical reactions and fluorophore quenching through interactions with nitroxide' s unpaired electron. Thus, the aim here was to demonstrate a useful detection method for lipid-derived radicals in in vivo.

Research Products

• [Journal Article] Structure-Reactivity Relationship of Piperidine Nitroxide : Electrochemical, ESR and Computational Studies (2011)
  • Author(s): Toshihide Yamasaki, et al
  • Journal Title: J.Organic Chemistry Volume: 76 Issue: 2 Pages: 435-440
  • DOI: 10.1021/jo101961m
  • Peer Reviewed
• [Journal Article] Structural Concept of Nitroxide As a Lipid Peroxidation Inhibitor (2011)
  • Author(s): Yamasaki T, et al
  • Journal Title: The Journal of Organic Chemistry Volume: 76(10) Issue: 10 Pages: 4144-4148
  • DOI: 10.1021/jo200361p
  • Peer Reviewed
• [Journal Article] Oxygen concentration dependence of lipid peroxidation and lipid-derived radical generation : application of profluorescent nitroxide switch (2011)
  • Author(s): Mito F, Kitagawa K, Yamasaki T, Shirahama C, Oishi T, Ito Y, Yamato M, Yamada K
  • Journal Title: Free Radic Res Volume: 45(9) Issue: 9 Pages: 1103-10
  • DOI: 10.3109/10715762.2011.595410
  • Peer Reviewed
• [Journal Article] Structure-reactivity relationship of piperidine nitroxide : electrochemical, ESR and computational studies (2011)
  • Author(s): Yamasaki T, et al.
  • Journal Title: The Journal of Organic Chemistry Volume: 76 Pages: 435-440
  • Peer Reviewed
• [Journal Article] In vivo evaluation of novel nitroxyl radicals with reduction stability (2010)
  • Author(s): Kinoshita Y, Yamada K, Yamasaki T, Mito F, Yamato M, Kosem N, Deguchi H, Shirahama C, Ito Y, Kitagawa K, Okukado N, Sakai K, Utsumi H
  • Journal Title: Free Radic Biol Med Volume: 49(11) Pages: 1703-9
  • URL: http://dx.doi.org/10.1016/j.freeradbiomed.2010.08.027
  • Peer Reviewed
• [Journal Article] In vivo evaluation of novel nitroxyl radicals with reduction stability. (2010)
  • Author(s): Kinoshita Y, et al.
  • Journal Title: Free Radical Biology & Medicine Volume: 49 Pages: 1703-1709
  • Peer Reviewed
• [Presentation] In vivo evaluation of novel nitroxyl radicals (nitroxide) with reduction stability (2011)
  • Author(s): Kenichi Yamada
  • Organizer: International Symposium of Free Radical Research : Contribution to Medicine
  • Place of Presentation: 京都
  • Year and Date: 2011-01-21
• [Presentation] Lipid-derived radical detection method : profluorescent nitroxide switching (2011)
  • Author(s): Yamada K, Matsuoka Y, Yamasaki T, Mito F, Kitagawa K, Oishi T, Ito Y, Shirahama C, Yamato M
  • Organizer: Society for Free Radical Biology and Medicine 18^<th> Annual Meeting, Atlanta
  • Place of Presentation: USA November
• [Presentation] Development and Application ofα-Substituented Piperidine Nitroxides. International conference devoted to Synthesis, Properties and Implications of Nitroxides (2011)
  • Author(s): Yamada K, Yamasaki T, Mito F
  • Organizer: Marseilles, France
  • Place of Presentation: Marseilles, France
• [Presentation] Utsumi H. In vivo evaluation of novel nitroxyl radicals(nitroxide) with reduction stability (2011)
  • Author(s): Yamada K, Kinoshita Y, Yamasaki T, Mito F, Yamato M, Ito Y, Kitagawa K, Kosem N, Pandian S, Nakano K, Murugesan R, Okukado N, Sakai K
  • Organizer: International Symposium of Free Radical Research : Contribution to Medicine
  • Place of Presentation: Kyoto
• [Presentation] General consideration of ascorbic acid resistance nitroxide (2010)
  • Author(s): Yamada K, Kinoshita Y, Yamasaki T, Mito F, Yamato M, Kosem I, Deguchi H, Shirahama C, Ito Y, Okukado N, Sakai K, Utsumi H
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii, USA