Formulation studies on neuropeptides for type-2 diabetes treatment

• Project/Area Number: 22790043
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Physical pharmacy
• Research Institution: University of Shizuoka
• Principal Investigator: ONOUE Satomi 静岡県立大学, 薬学部, 准教授 (00457912)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ドラッグデリバリー / ペプチド創薬 / ペプチド創剤 / 糖尿病 / 2型糖尿病 / 神経ペプチド / インスリン分泌促進 / アポトーシス

Research Abstract

The present study aimed to develop new therapeutic agent to treat type 2 diabetes with a focus on glucagon-secretin family neuropeptides. Biochemical studies and structure-activity relationship studies were undertaken to provide new promising derivatives of neuropeptides. To improve therapeutic compliance for neuropeptides, sustained-release dry powder formulation system was also established with use of biodegradable polymer.

Research Products

• [Journal Article] Strategic Formulation Study on Dry Powder Inhalation System Based on Modulated Molecular Properties and Controlled Pharmacokinetics (2013)
  • Author(s): Onoue S
  • Journal Title: YAKUGAKU ZASSHI Volume: 133 Issue: 1 Pages: 93-98
  • DOI: 10.1248/yakushi.12-00209
  • NAID: 130003361875
  • ISSN: 0031-6903, 1347-5231
  • Year and Date: 2013-01-01
  • Peer Reviewed
• [Journal Article] Novel solid self-emulsifying drug delivery system of coenzyme Q₁₀ with improved photochemical and pharmacokinetic behaviors. (2012)
  • Author(s): Onoue S, Uchida A, Kuriyama K, Nakamura T, Seto Y, Kato M, Hatanaka J, Tanaka T, Miyoshi H, Yamada S.
  • Journal Title: Eur J Pharm Sci. Volume: 46 Issue: 5 Pages: 492-9
  • DOI: 10.1016/j.ejps.2012.03.015
  • Peer Reviewed
• [Journal Article] Inhalable sustained-release formulation of long-acting vasoactive intestinal peptide derivative alleviates acute airway inflammation. (2012)
  • Author(s): Onoue S, Matsui T, Kuriyama K, Ogawa K, Kojo Y, Mizumoto T, Karaki S, Kuwahara A, Yamada S.
  • Journal Title: Peptides Volume: 35 Issue: 2 Pages: 182-9
  • DOI: 10.1016/j.peptides.2012.03.021
  • Peer Reviewed
• [Journal Article] Inhalable dry-emulsion formulation of cyclosporine A with improved anti-inflammatory effects in experimental asthma/COPD-model rats. (2012)
  • Author(s): Onoue S, Sato H, Ogawa K, Kojo Y, Aoki Y, Kawabata Y, Wada K, Mizumoto T, Yamada S.
  • Journal Title: Eur J Pharm Biopharm. Volume: 80 Issue: 1 Pages: 54-60
  • DOI: 10.1016/j.ejpb.2011.10.003
  • Peer Reviewed
• [Journal Article] Development of PACAP38 Analogue with Improved Stability: Physicochemical and In Vitro/In Vivo Pharmacological Characterization. (2011)
  • Author(s): Satomi Onoue, Junko Hanato, Kazuki Kuriyama, Takahiro Mizumoto, Shizuo Yamada
  • Journal Title: J. Mol.Neurosci Volume: 43 Pages: 85-93
• [Journal Article] Novel vasoactive intestinal peptide derivatives with improved stability protect rat alveolar L2 cells from cigarette smoke-induced cytotoxicity and apoptosis. (2011)
  • Author(s): Shingen Misaka, Hideyuki Sato, Yosuke Aoki, Takahiro Mizumoto, Satomi Onoue, Shizuo Yamada
  • Journal Title: Peptides Volume: 32 Pages: 401-407
• [Journal Article] Inhalable sustained-release formulation of glucagon: in vitro amyloidogenic and inhalation properties, and in vivo absorption and bioactivity. (2011)
  • Author(s): Satomi Onoue, Kazuki Kuriyama, Atsushi Uchida, Takahiro Mizumoto, Shizuo Yamada
  • Journal Title: Pharm. Res. Volume: 28 Pages: 1157-1166
• [Journal Article] Formulation design and in vivo evaluation of dry powder inhalation system of new vasoactive intestinal peptide derivative ([R15,20,21, L17, A24,25, des-N28]-VIP-GRR) in experimental asthma/COPD model rats. (2011)
  • Author(s): Satomi Onoue, Yoshiki Aoki, Takuya Matsui, Yoshiki Kojo, Shingen Misaka, Takahiro Mizumoto, Shizuo Yamada
  • Journal Title: IntJ Pharm Volume: 410 Pages: 54-60
• [Journal Article] Development of high-energy amorphous solid dispersion of nanosized nobiletin, a citrus polymethoxylated flavone, with improved oral bioavailability. (2011)
  • Author(s): Satomi Onoue, ほか
  • Journal Title: Journal of Pharmaceutical Sciences Volume: (In press)
  • Peer Reviewed
• [Journal Article] Development of (-)-epigallocatechin-3-gallate (EGCG)-loaded enteric microparticles with intestinal mucoadhesive property. (2011)
  • Author(s): Satomi Onoue, ほか
  • Journal Title: International Journal of Pharmaceutics Volume: 410 Pages: 111-113
  • Peer Reviewed
• [Journal Article] Formulation design and in vivo evaluation of dry powder inhalation system of new vasoactive intestinal peptide derivative ([R^<15,20,21>, L^<17>, A^<24,25>, des-N^<28>]-VIP-GRR) in experimental asthma/COPD model rats. (2011)
  • Author(s): Satomi Onoue, ほか
  • Journal Title: International Journal of Pharmaceutics Volume: 410 Pages: 54-60
  • Peer Reviewed
• [Journal Article] Inhalable sustained-release formulation of glucagon : in vitro amyloidogenic and inhalation properties, and in vivo absorption and bioactivity. (2011)
  • Author(s): Satomi Onoue, ほか
  • Journal Title: Pharmaceutical Research Volume: 28 Pages: 1157-1166
  • Peer Reviewed
• [Journal Article] Stable dry powder inhaler formulation of tranilast attenuated antigen-evoked airway inflammation in rats. (2011)
  • Author(s): Yohei Kawabata, Satomi Onoue, ほか
  • Journal Title: European Journal of Pharmaceutics and Biophar maceutics Volume: 77 Pages: 178-181
  • Peer Reviewed
• [Journal Article] Development of PACAP38 analogue with improved stability : physicochemical and in vitro/in vivo pharmacological characterization. (2011)
  • Author(s): Satomi Onoue, ほか
  • Journal Title: Journal of Molecular Neuroscience Volume: 43 Pages: 85-93
  • Peer Reviewed
• [Journal Article] Inhalable powder formulation of a stabilized vasoactive intestinal peptide (VIP) derivative: anti-inflammatory effect in experimental asthmatic rats (2010)
  • Author(s): Misaka Shingen, Yosuke Aoki, Shin-ichiro Karaki, Atsukazu Kuwahara, Satomi Onoue, Shizuo Yamada
  • Journal Title: Peptides Volume: 31 Pages: 72-78
• [Journal Article] Inhalable powder formulation of vasoactive intestinal peptide derivative, [R15,20,21, L17]-VIP-GRR, attenuated neutrophilic airway inflammation in cigarette smoke-exposed rats. (2010)
  • Author(s): Satomi Onoue, Shingen Misaka, Yosuke Aoki, Shinichiro Karaki, Atzukazu Kuwahara, Asami Ohide, Takahiro Mizumoto, and Shizuo Yamada
  • Journal Title: Eur. J.Pharm. Sci. Volume: 41 Pages: 508-514
  • NAID: 10028237981
• [Journal Article] Inhalable powder formulation of vasoactive intestinal peptide derivative, [R^<15,20,21>, L^<17>]-VIP-GRR, attenuated neutrophilic airway inflammation in cigarette smoke-exposed rats. (2010)
  • Author(s): Satomi Onoue, ほか
  • Journal Title: European Journal of Pharmaceutical Sciences Volume: 41 Pages: 508-514
  • NAID: 10028237981
  • Peer Reviewed
• [Journal Article] Improved dissolution and pharmacokinetic behavior of cyclosporine A using high-energy amorphous solid dispersion approach. (2010)
  • Author(s): Satomi Onoue, ほか
  • Journal Title: International Journal of Pharmaceutics Volume: 399 Pages: 94-101
  • NAID: 130004639881
  • Peer Reviewed
• [Presentation] 物性ならびに薬物動態制御を基板とした薬物送達研究 (2012)
  • Author(s): 尾上誠良
  • Organizer: 日本薬剤学会 第 27 年会
  • Place of Presentation: 神戸
  • Invited
• [Presentation] Development of Inhalable Sustained-Release Formulation of Glucagon. (2011)
  • Author(s): Satomi Onoue, Kazuki Kuriyama, Atsushi Uchida, Takahiro Mizumoto, Shizuo Yamada
  • Organizer: The 2011 AAPS Annual Meeting and Exposition
  • Place of Presentation: Washington D.C., US
  • Year and Date: 2011-10-25
• [Presentation] Nano-crystalline solid dispersion system of poorly-soluble drugs for in halation therapy (2010)
  • Author(s): 尾上誠良
  • Organizer: International biomedicine and technology&health care management summit
  • Place of Presentation: Jiangyin, China
  • Year and Date: 2010-11-27
• [Presentation] Development of Nano-crystalline Solid Dispersion of Tranilast for Inhalation Therapy of Airway Inflammatory Diseases (2010)
  • Author(s): 尾上誠良, ほか
  • Organizer: FIP PSWC 2010/AAPS Annual Meeting and Exposition
  • Place of Presentation: New Orleans, US
  • Year and Date: 2010-11-17
• [Presentation] Phrmacokinetics and pharmacodynamics of glucagon-loaded PLGA nanospheres for inhalation therapy. (2010)
  • Author(s): 栗山和樹, 尾上誠良, ほか
  • Organizer: 25^<th> JSSX Annual Meeting in Tokyo
  • Place of Presentation: 東京
  • Year and Date: 2010-10-08
• [Presentation] Neurotrophic activities of newly developed pituitary adenylate cyclase activating polypeptide derivatives in central and peripheral nervous systems. (2010)
  • Author(s): Satomi Onoue
  • Organizer: The 3rd IANRAC hosted by International Association of Neurorestoratology
  • Place of Presentation: Beijing, China
• [Presentation] Phrmacokinetics and pharmacodynamics of glucagon-loaded PLGA nanospheres for inhalation therapy. (2010)
  • Author(s): Kazuki Kuriyama, Atsushi Uchida, Takahiro Mizunoto, Satomi Onoue and Shizuo Yamada
  • Organizer: 25th JSSX Annual Meeting in Tokyo.
  • Place of Presentation: Tokyo
• [Presentation] Inhalable powder formulation of vasoactive intestinal peptidede rivative, [R15, 20,21, L17]-VIP-GRR, attenuated neutrophilic airway inflammation in cigarette smoke-exposed rats. (2010)
  • Author(s): Yosuke Aoki, Shingen Misaka, Takahiro Mizumoto, Satomi Onoue
  • Organizer: 5th International peptide symposium
  • Place of Presentation: Kyoto
• [Presentation] Inhalable powder formulation of stabilized vasoactive intestinal peptide derivative (IK312548). (2010)
  • Author(s): Yoshiki Kojo, Yosuke Aoki, Shingen Misaka, Takuya Matsui, Takahiro Mizumoto, Satomi Onoue
  • Organizer: 5th International peptide symposium
  • Place of Presentation: Kyoto
• [Presentation] Development of glucagon-loaded PLGA nanospheres for inhalation therapy with improved pharmacokinetics and pharmacodynamics. (2010)
  • Author(s): Kazuki Kuriyama, Atsushi Uchida, Takahiro Mizumoto, Satomi Onoue
  • Organizer: 5th International peptide symposium
  • Place of Presentation: Kyoto
• [Remarks] ホームページにおいて研究成果報告
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/~yakuzai/6