Studies on the assembly of the proteasome

• Project/Area Number: 22790060
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: KANEKO Takeumi 東京大学, 大学院・薬学系研究科, 助教 (80508135)
• Research Collaborator: MURATA Shigeo 東京大学, 大学院・薬学系研究科, 教授 (20344070)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: プロテアソーム / ユビキチン / タンパク質分解 / 分子集合 / 蛋白質分解

Research Abstract

The 26S proteasome is an about 2. 5 MDa large protein complex consisting of a catalytic 20S proteasome and one or two 19S regulatory particles. More than 70 subunits are gathered to form the 26S proteasome. To reveal how such a large complex is constructed, we focused on the assembly mechanism of the lid subcomplex, which has not yet been examined precisely. Our research revealed that the lid is assembled in a well-ordered way, and the assembly of the lid is completed by the integration of Rpn12.

Research Products

• [Journal Article] Using siRNA techniques to dissectproteasome assembly pathways in mammaliancells (2012)
  • Author(s): Kaneko T, Murata S
  • Journal Title: Methods Mol Biol Volume: 832 Pages: 433-42
  • Peer Reviewed
• [Journal Article] Proteasome assembly defect due to aproteasome subunit beta type 8(PSMB8) mutation causes the autoinflammatorydisorder, Nakajo-Nishimura syndrome (2011)
  • Author(s): Arima K, Kinoshita A, Mishima H, Kanazawa N, Kaneko T, Mizushima T, Ichinose K, Nakamura H, Tsujino A, Kawakami A, Matsunaka M, Kasagi S, KawanoS, Kumagai S, Ohmura K, Mimori T, HiranoM, Ueno S, Tanaka K, Tanaka M, ToyoshimaI, Sugino H, Yamakawa A, Tanaka
  • Journal Title: ProcNatl Acad Sci U S A Volume: 108(36) Pages: 14914-9
  • Peer Reviewed
• [Presentation] The assembly pathway of the mammalian proteasome lid subcomplex (2010)
  • Author(s): 金子岳海
  • Organizer: PPDUP 2010
  • Place of Presentation: バンクーバー、カナダ
  • Year and Date: 2010-05-08
• [Presentation] The assemblypathway of the mammalian proteasome lid subcomplex PPDUP (2010)
  • Author(s): 金子岳海、村田茂穂
  • Place of Presentation: バンクーバー、カナダ
• [Remarks]
  • URL: http://www.f.u-tokyo.ac.jp/~tanpaku/index.html
• [Remarks]
  • URL: http://www.f.u-tokyo.ac.jp/~tanpaku/index.html
• [Remarks]
  • URL: http://www.f.u-tokyo.ac.jp/~tanpaku/