Identification and functional analysis of two kinds of novel Chlamydphila pneumoniae effectors

• Project/Area Number: 22790419
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Kawasaki Medical School
• Principal Investigator: YANATORI Izumi 川崎医科大学, 医学部, 助教 (40454847)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 病原性 / 肺炎クラミジア / エフェクター / 感染制御 / III型分泌装置

Research Abstract

In this research, we identified two novel Chlamydophila pneumonia specific effectors, Fke034 and Fke063. Functional analysis revealed that Fke034 interacts with PACSIN2 and might control the function of PACSIN2 in the host cells, and Fke063 is localized at Cajal body and might affect the intracellular vesicle trafficking system.

Research Products

• [Journal Article] Heme and non-heme iron transporters in non-polarized and polarized cells (2010)
  • Author(s): Yanatori. I
  • Journal Title: BMC cell biology Volume: 11 Pages: 39-39
  • Peer Reviewed
• [Journal Article] 小腸における鉄吸収メカニズム (2010)
  • Author(s): 岸文雄
  • Journal Title: 日本医師会雑誌 Volume: 139 Pages: 291-294
• [Presentation] Genomic screening for the novel effector molecules in Chlamydophila pneumoniae using growth inhibitory effect in yeast cells. (2013)
  • Author(s): Yanatori I., Yasui Y., Miura K., Kishi K
  • Organizer: 6th Biennial Meeting of the Chlamydia Basic Research Society
  • Place of Presentation: San Antonio (USA)
  • Year and Date: 2013-03-19
• [Presentation] Genomic screening for the novel effector molecules in Chlamydophila pneumoniae using growth inhibitory effect in yeast cells (2013)
  • Author(s): 簗取いずみ
  • Organizer: 6th Biennial Meeting of the Chlamydia Basic Research Society
  • Place of Presentation: The Menger Hotel (Texas)
• [Presentation] Genomic screening for Chlamydophila pneumoniae antigens using sera from the patients (2013)
  • Author(s): 安井ゆみこ
  • Organizer: 6th Biennial Meeting of the Chlamydia Basic Research Society
  • Place of Presentation: The Menger Hotel (Texas)
• [Presentation] 肺炎クラミジアの新規エフェクターFke034は宿主細胞の細胞膜直下に局在する (2013)
  • Author(s): 三浦公志郎
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ国際会議場（千葉県）
• [Presentation] Characterization on an effector candidate Fke063 in Chlamydophila pneumonia (2011)
  • Author(s): Kawai Y., Miura K., Katayama A., YasuiY., Yanatori I., Ouchi K. and Kishi F
  • Organizer: International Union of Microbiological Societies 2011Congress
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-07
• [Presentation] CHARACTERIZATION OF AN EFFECTOR CANDIDATE FKE063 IN CHLAMYDOPHILA PINEUMONIAE (2011)
  • Author(s): Kawai. Y, et al
  • Organizer: International Union of Microbiological Societies (IUMS) 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-06
• [Presentation] Screenig and analysis of effector candidate molecules from Chlamydophila pneumonia genome (2011)
  • Author(s): Miura K., Kawai Y., Katayama A.,Yanatori I., Yasui Y., Kishi F
  • Organizer: The111th ASM General Meeting.
  • Place of Presentation: New Orleans (USA)
  • Year and Date: 2011-05-24
• [Presentation] Genome-wide screening of pathogen effectors in Chlamydohila pneumoniae using a yeast expression system (2010)
  • Author(s): 河合泰宏
  • Organizer: Twelfth Interllational Symposium on Human Chlamydial Infections
  • Place of Presentation: Salzburg, Austria
  • Year and Date: 2010-06-24