Molecular mechanism of rolling circle DNA replication of human papillomavirus

• Project/Area Number: 22790443
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: MATSUO Rika (KUSUMOTO Rika) 国立感染症研究所, 病原体ゲノム解析研究センター, 主任研究官 (90514133)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ウイルス / がん / DNA 複製 / パピローマウイルス / 複製 / キナーゼ / DNA複製

Research Abstract

To elucidate the mechanism of DNA replication of human Papillomavirus, we searched for a factor that affects the virus DNA replication in cells. We performed ALPHA screening and identified ten tyrosine kinases that bind to HPV16 E1. We also identified peptides phosphorylated by an unknown kinase bound to E1 among a set of tyrosine kinase substrate peptides, and speculated seven kinases. Interaction between these kinases and E1 in cells and the effects of the kinases to HPV DNA replication will be tested.

Research Products

• [Journal Article] Genotype distribution of human papillomaviruses in Japanese women with abnormal cervical cytology (2012)
  • Author(s): Kondo K, Uenoyama A, Kitagawa R, Tsunoda H, Kusumoto-Matsuo R, Mori S, Ishii Y, Takeuchi T, Kanda T, Kukimoto I
  • Journal Title: Open Virol J Volume: 6 Issue: 1 Pages: 277-283
  • DOI: 10.2174/1874357901206010277
  • Peer Reviewed
• [Journal Article] Novel multiplexed genotyping of human papillomavirus using a VeraCode-allele-specific primer extension method. (2012)
  • Author(s): Kitamura-Muramatsu Y., Kusumoto-Matsuo R., and Kukimoto I.
  • Journal Title: Microbiol Immunol (Wiley-Blackwell) Volume: 56 Issue: 2 Pages: 128-133
  • DOI: 10.1111/j.1348-0421.2011.00406.x
  • Peer Reviewed
• [Journal Article] Biased amplification of human papillomavirus DNA in specimens containing multiple human papillomavirus types by PCR with consensus primers. (2011)
  • Author(s): Mori S., Nakao S., Kukimoto I., Kusumoto-Matsuo R., Kondo K. and Kanda T
  • Journal Title: Cancer Sci (Blackwell Publishing) Volume: 102 Issue: 6 Pages: 1223-1227
  • DOI: 10.1111/j.1349-7006.2011.01922.x
• [Journal Article] Rolling circle replication of human papillomavirus type 16 DNA in epithelial cell extracts. (2011)
  • Author(s): Kusumoto-Matsuo R., Kanda T. and Kukimoto I
  • Journal Title: Genes Cells (Blackwell Science Ltd.) Volume: 16 Issue: 1 Pages: 23-33
  • DOI: 10.1111/j.1365-2443.2010.01458.x
• [Journal Article] Rolling circle replication of human papillomavirus type 16 DNA in epithelial cell extracts. (2011)
  • Author(s): Kusumoto-Matsuo R, Kanda T, Kukimoto I.
  • Journal Title: Genes to Cells Volume: 16 Pages: 23-33
  • Peer Reviewed
• [Journal Article] Biased amplification of human papillomavirus DNA in specimens containing multiple human papillomavirus types by PCR with consensus primers. (2011)
  • Author(s): Mori S, Nakao S, Kukimoto I, Kusumoto-Matsuo R, Kondo K, Kanda T.
  • Journal Title: Cancer Science Volume: 102 Pages: 1223-1227
  • Peer Reviewed
• [Journal Article] Cooperation of DNA-PKcs and WRN helicase in the maintenance of telomeric D-loops. (2010)
  • Author(s): Kusumoto-Matsuo R., Opresko PL., Tahara H. and Bohr, VA
  • Journal Title: Aging (Albany NY) (Impact Journals) Volume: 2 Pages: 274-284
  • URL: http://www.impactaging.com/papers/v2/n5/full/100141.html