Regulation of autoimmune response by an Ig domain-containing sialomucin, nepmucin

• Project/Area Number: 22790466
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Osaka University
• Principal Investigator: UMEMOTO Eiji 大阪大学, 医学系・研究科, 助教 (90452440)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 免疫寛容・自己免疫 / 高内皮性静脈 / リンパ球トラフィッキング / 接着分子 / 白血球移動 / 高内皮細静脈 / 自己免疫応答 / 免疫学 / リンパ球動態 / アポトーシス

Research Abstract

Nepmucin is an Ig-domain-containing adhesion molecule expressed in certain types of vascular endothelial cells and promotes lymphocyte transendothelial migration in vitro. We found that nepmucin binds to phosphatidylserine, which appears on the outer leaflet of the plasma membrane in apoptotic cells. Nepmucin promotes the uptake of apoptotic cells by endothelial cells of lymph node high endothelial venule. Nepmucin-deficient mice showed elevated autoantibody production in an age-dependent manner. Collectively, our findings indicate that nepmucin is involved in autoimmune response.

Research Products

• [Journal Article] Constitutive plasmacytoid dendritic cell migration to the splenic white pulp is cooperatively regulated by CCR7-and CXCR4-mediated signaling (2012)
  • Author(s): *Umemoto E., * Otani K., Ikeno T., Garcia N. V., Hayasaka H., Bai Z., Jang M. H., Tanaka T., Nagasawa T., Ueda K., & Miyasaka M.(* equal contribution)
  • Journal Title: J. Immunol Volume: (In press)
  • Peer Reviewed
• [Journal Article] SIRPτ/ CD172a Regulates Eosinophil Homeostasis (2011)
  • Author(s): Garcia N. V., Umemoto E., Saito Y., Yamasaki M., Hata E., Matozaki T., Murakami M., Jung Y. J., Woo S. Y., Seoh J. Y., Jang M. H., Aozasa K., & Miyasaka M.
  • Journal Title: J. Immunol Volume: 187 Pages: 2268-2277
  • Peer Reviewed
• [Journal Article] Novel Regulators of Lymphocyte Trafficking across High Endothelial Venules (2011)
  • Author(s): Umemoto, E., Hayasaka H., Bai Z., Cai L., Yonekura S., Peng X., Takeda A., Tohya K. & Miyasaka M.
  • Journal Title: Crit. Rev. Immunol Volume: 31 Pages: 147-169
  • Peer Reviewed
• [Journal Article] Novel regulators of lymphocyte trafficking across high endothelial venule (2011)
  • Author(s): Umemoto E., Hayasaka H., et al
  • Journal Title: Critical Reviews in Immunology Volume: 31 Pages: 147-169
  • Peer Reviewed
• [Journal Article] SIRPα/CD172a regulates eosinophil homeostasis (2011)
  • Author(s): Verjan G.M., Umemoto, et al
  • Journal Title: The Journal of Immunology Volume: 187 Pages: 2268-2277
  • Peer Reviewed
• [Journal Article] Microanatomy of lymphocyte-endothelial interactions at the high endothelial venules of lymph nodes (2010)
  • Author(s): Tohya K., Umemoto E., & Miyasaka M.
  • Journal Title: Histol. Histopathol Volume: 25 Pages: 781-794
  • Peer Reviewed
• [Journal Article] Microanatomy of lymphocyte-endothelial interactions at the high endothelial venules of lymph nodes (2010)
  • Author(s): Tohya K., Umemoto E., et al.
  • Journal Title: Histology and Histopathology Volume: 25 Pages: 781-794
  • Peer Reviewed
• [Presentation] Constitutive pDC migration to the splenic white pulp is cooperatively regulated by CCR7 ligands and CXCL12 (2012)
  • Author(s): 梅本英司
  • Organizer: Keystone Symposia
  • Place of Presentation: Breckenridge, Colorado
  • Year and Date: 2012-01-11
• [Presentation] Constitutive pDC migration to the splenic white pulp is cooperatively regulated by CCR7 ligands and CXCL12 (2012)
  • Author(s): Umemoto E. et al.
  • Organizer: Keystone Symposia
  • Place of Presentation: Colorado, USA
• [Presentation] Constitutive pDC migration to the splenic white pulp is cooperatively regulated by CCR7 ligands and CXCL12 (2011)
  • Author(s): 梅本英司
  • Organizer: 第40回日本免疫学会総会・学術集会
  • Place of Presentation: 千葉
  • Year and Date: 2011-11-29
• [Presentation] Constitutive pDC migration to the splenic white pulp is cooperatively regulated by CCR7 ligands and CXCL12 (2011)
  • Author(s): 梅本英司, 他
  • Organizer: 第40回日本免疫学会
  • Place of Presentation: 千葉
• [Presentation] Nepmucin/ CD300LG promotes LPA-induced lymphocyte transcellular migration across HEV endothelial cells (2011)
  • Author(s): 竹田彰、梅本英司, 他
  • Organizer: 第40回日本免疫学会
  • Place of Presentation: 千葉
• [Presentation] The role of PV-1 and neuropilin-1 in lymphocyte transendothelial migration across HEVs (2011)
  • Author(s): 池野嵩、梅本英司, 他
  • Organizer: 第40回日本免疫学会
  • Place of Presentation: 千葉
• [Presentation] Signal regulating protein alpha(SIRPτ)/ CD172a regulates eosinophil homeostasis (2011)
  • Author(s): Verjan Garcia N, 梅本英司, 他
  • Organizer: 第40回日本免疫学会
  • Place of Presentation: 千葉
• [Presentation] In vivo inhibition of the autotaxin/ LPA axis results in inhibition of lymphocyte transmigration across high endothelial venules (2011)
  • Author(s): 秦枝里奈、白忠彬、蔡林君、梅本英司, 他
  • Organizer: 第40回日本免疫学会
  • Place of Presentation: 千葉
• [Presentation] HEV上の新しいシアロムチンnepmucin (2010)
  • Author(s): 梅本英司、宮坂昌之
  • Organizer: 第34回日本リンパ学会総会
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2010-06-26
• [Presentation] Nepmucin mediates uptake of apoptotic cells in the endothelial cells of high endothelial venules (2010)
  • Author(s): 梅本英司, 他
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸
• [Presentation] SIRPτ/ SHPS-1 is strongly expressed in mouse eosinophils (2010)
  • Author(s): Verjan Garcia N, 梅本英司, 他
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸
• [Presentation] The role of a novel HEV-associated sialomucin, nepmucin (2010)
  • Author(s): 梅本英司、宮坂昌之
  • Organizer: 第34回日本リンパ学会
  • Place of Presentation: 東京
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/orgctl/www/index-jp.html