Analysis of BCR degradation by a lysosomal protein LAPTM5

• Project/Area Number: 22790482
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: OUCHIDA Rika 独立行政法人理化学研究所, 免疫多様性研究チーム, 研究員 (80391887)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: リソソーム / B細胞抗原受容体 / B細胞活性化 / 自己免疫疾患 / B cell receptor(BCR) / lysosome / B cell activation / B cell receptor (BCR)

Research Abstract

Modulation of antigen receptor expression is an important mechanism for the regulation of immune responses. We demonstrate that LAPTM5 regulates BCR levels and immune response by BCR degradation in lysosomes and LAPTM5-mediated BCR downmodulation is an important mechanism to prevent B cell hyperactivation and autoantibody production.

Research Products

• [Journal Article] Rapid cell division contributes to efficient induction of A/T mutations during Ig gene hypermutation (2011)
  • Author(s): 狩野智恵、大内田理佳、古久保哲朗、王継揚
  • Journal Title: Molecular Immunology Volume: 48巻 Pages: 1993-1999
  • Peer Reviewed
• [Journal Article] Rapid cell division contributes to efficient induction of A/T mutations during Ig gene hypermutation (2011)
  • Author(s): Kano C, Ouchida R, Kokubo T, Wang J-Y
  • Journal Title: Mol.Immunol. Volume: 48(15-16) Pages: 1993-1999
  • Peer Reviewed
• [Journal Article] A role for lysosomal-associated protein transmembrane 5 in the negative regulation of surface B cell receptor levels and B cell activation (2010)
  • Author(s): 大内田理佳、黒崎知博、王継揚
  • Journal Title: The Journalof Immunology Volume: 185巻 Pages: 294-301
  • Peer Reviewed
• [Journal Article] A role for lysosomal-associated protein transmembrane 5 in the negative regulation of surface B cell receptor levels and B cell activation. (2010)
  • Author(s): Rika Ouchida
  • Journal Title: The Journal of Immunology Volume: 185 Pages: 294-301
  • Peer Reviewed
• [Presentation] The lysosomal protein LAPTM5 negatively regulates B cell activation and antibody production by downmodulating cell surface BCR expression (2010)
  • Author(s): Rika Ouchida
  • Organizer: 第14回 国際免疫学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-23
• [Presentation] The lysosomal protein LAPTM5 negatively regulates B cell activation and antibody production by downmodulating cell surface BCR expression (2010)
  • Author(s): 大内田理佳
  • Place of Presentation: 神戸
• [Remarks]
  • URL: http://dx.doi.org/10.1016/j.molimm.2011.06.218