Establishment of hepatic stellate cell de-activation in liver fibrosis model using 3 dimensional bio artificial liver
| Project/Area Number |
22790673
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 病態モデル / 人工臓器工学 / 肝線維化 / 肝星細胞 / バイオ人工肝臓 / 星細胞 / 3次元培養 |
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| Research Abstract |
Bio artificial liver model which is co-cultured with immortal mouse stellate cell, endothelial cell and hepatocyte using radial flow bioreactor showed activity of liver fibrosis in conventional culture fluid(2% FBS+ASF-104N). When an anti-fibrotic agent was added in this bio artificial liver model, LRAT which reflect ability to store vitamin A into stellate cell was re-expressed in mRNA and protein level.
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Report
(3 results)
Research Products
(5 results)
