Establishment of hepatic stellate cell de-activation in liver fibrosis model using 3 dimensional bio artificial liver

• Project/Area Number: 22790673
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Jikei University School of Medicine
• Principal Investigator: NAGATSUMA Keisuke 東京慈恵会医科大学, 医学部, 助教 (40543658)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 病態モデル / 人工臓器工学 / 肝線維化 / 肝星細胞 / バイオ人工肝臓 / 星細胞 / 3次元培養

Research Abstract

Bio artificial liver model which is co-cultured with immortal mouse stellate cell, endothelial cell and hepatocyte using radial flow bioreactor showed activity of liver fibrosis in conventional culture fluid(2% FBS+ASF-104N). When an anti-fibrotic agent was added in this bio artificial liver model, LRAT which reflect ability to store vitamin A into stellate cell was re-expressed in mRNA and protein level.

Research Products

• [Journal Article] Free fatty acids induce transglutaminase 2-dependent apoptosis in hepatocytes via ER stress-stimulated PERK pathways (2012)
  • Author(s): Kuo TF, Tatsukawa H, Nagatsuma K, Matsuura T, Kojima S.
  • Journal Title: J Cell Physiol Volume: 227 Issue: 3 Pages: 1130-7
  • DOI: 10.1002/jcp.22833
  • Peer Reviewed
• [Journal Article] Transplantation of liver organoids in the omentum and kidney (2011)
  • Author(s): Saito R、Ishii Y、Ito R、Nagatsuma K、Tanaka K、Saito M、Maehashi H、Nomoto H、Ohkawa K、Mano H、Aizawa M、Hano H、Yanaga K、Matsuura T
  • Journal Title: Artif Organs Volume: Vol.35, No.1 Issue: 1 Pages: 80-83
  • DOI: 10.1111/j.1525-1594.2010.01049.x
  • Peer Reviewed
• [Presentation] TRECK急性肝不全モデルマウスにおける肝組織TGF-β活性化反応の観察 (2011)
  • Author(s): 永妻啓介、松浦知和、小嶋聡一、羽野寛
  • Organizer: 第25回肝類洞壁細胞研究会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2011-12-18
• [Presentation] TRECK急性肝不全モデルマウスにおける肝組織TGF-β活性化反応の観察 (2011)
  • Author(s): 永妻啓介、松浦知和、小嶋聡一、羽野寛
  • Organizer: 第37回日本急性肝不全研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-01
• [Presentation] TGF-β Activation in the Liver during Acute Hepatic Failure induced in TRECK Mice (2011)
  • Author(s): 永妻啓介、松浦知和、小嶋聡一
  • Organizer: 16th International Symposium on Cells of the Hepatic Sinusoid
  • Place of Presentation: イタリア・フィレンツェ