• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of the biomarker in urine of cardiovascular and kidney disease.

Research Project

Project/Area Number 22790715
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionUniversity of Miyazaki

Principal Investigator

NAGATA Sayaka  宮崎大学, 医学部, 助教 (00452920)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords臨床心血管病態学 / バイオマーカー / 生理活性ペプチド / レニン・アンジオテンシン系 / プロアンジオテンシン-12 / 循環器疾患 / 腎臓疾患 / ラジオイムノアッセイ / レニン・アンジオテンシン / 高血圧 / 循環器・腎臓疾患 / 質量分析装置
Research Abstract

I isolated and identified a new angiotensinogen-derived peptide from the human urine. Then, I made antiserum to use rabbits for this peptide. And I established assay system. So, I collected urine samples and measured of this peptide by radioimmunoassay. I found that urinary new peptide/creatinin ratio was significantly higher than the estimated glomerular filtration rate (eGFR) < 30 ml/min/1.73 m2 group in other two groups (eGFR >90, 90-30 ml/min/1.73 m2). And, when compared with the protein urea plas in diabetes, the urinary new peptide/creatinin ratio was significantly higher than in protein urea minus group. In addition, urinary this peptide is associated renal derangement in patients with diabetic nephropathy. In addition, this peptide is abundant in a wide range of organs and tissues including the heart and kidney of human by immunohistochemistory.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (42 results)

All 2013 2012 2011 2010 Other

All Journal Article (18 results) (of which Peer Reviewed: 17 results) Presentation (18 results) Remarks (4 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Hemodynamic and hormonal changes to dual renin-angiotensin system inhibition in experimental hypertension2013

    • Author(s)
      Moniwa N, Varagic J, Ahmad S, VanCannon JL, Simington SW, Wang H, Groban L, Brosnihan KB, Nagata S, Kato J, Kitamura K, Gomez RA, SequeiraLopez ML, Ferrario CM
    • Journal Title

      Hypertension

      Volume: 61(2) Pages: 417-424

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hemodynamic and hormonal changes to dual renin-angiotensin system inhibition in experimental hypertension.2013

    • Author(s)
      Moniwa N
    • Journal Title

      Hypertension

      Volume: 61(2) Pages: 417-424

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasma and tissue concentrations of proangiotensin-12 in rats treated with inhibitors of the renin-angiotensin system2012

    • Author(s)
      Nagata S, Kato J, Kuwasako K, Kitamura K
    • Journal Title

      Hypertens Res

      Volume: 35 Pages: 234-238

    • NAID

      10030643913

    • Related Report
      2012 Final Research Report
  • [Journal Article] The third extracellular loop of the human calcitonin receptor-like receptor is crucial for the activation of adrenomedullin signalling2012

    • Author(s)
      Kuwasako K, Hay DL, Nagata S, Hikosaka T, Kitamura K, Kato J
    • Journal Title

      Brit J Pharmacol

      Volume: 166 Pages: 137-150

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of proangiotensin-12 infused continuously over 14 days in conscious rats2012

    • Author(s)
      KomatsuY, Kida N, Nozaki N, Kuwasako K, Nagata S, Kitamura K, Kato J
    • Journal Title

      Eur J Pharmacol

      Volume: 683 Pages: 186-189

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the single transmembrane domain of human receptor activity-modifying protein 3 in adrenomedullin receptor internalization2012

    • Author(s)
      Kuwasako K, Kitamura K, Nagata S, Nozaki N, Kato J
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 420 Pages: 582-587

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Circulation control by adrenomedullin 1 receptor complex2012

    • Author(s)
      Kuwasako K, Kitamura K, Nagata S, Kato J
    • Journal Title

      Nihon Yakurigaku Zasshi

      Volume: 140(1) Pages: 8-13

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neuromedin B stimulates proliferation of mouse chondrogenic cell line ATDC52012

    • Author(s)
      Saito H,Ikeda R, Inoue K, Nagata S, Kitamura K, Minamino N, Kangawa K, Miyata A
    • Journal Title

      Peptides

      Volume: 36(2) Pages: 299-302

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of proangiotensin-12 infused continuously over 14 days in conscious rats.2012

    • Author(s)
      Komatsu Y
    • Journal Title

      Eur J Pharmacol

      Volume: 683 Pages: 186-189

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasma and tissue concentrations of proangiotensin-12 in rats treated with inhibitors of the renin-angiotensin system2012

    • Author(s)
      Sayaka Nagata, et al
    • Journal Title

      Hypertension Research

      Volume: 35(2) Pages: 234-238

    • NAID

      10030643913

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure-function analysis of helix 8 of human calcitonin receptor-like receptor within the adrenomedullin 1 receptor2011

    • Author(s)
      Kuwasako K, Kitamura K, Nagata S, Hikosaka T, Kato J
    • Journal Title

      Peptides

      Volume: 32(1) Pages: 144-149

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Shared and separate functions of the RAMP-based adrenomedullin receptors2011

    • Author(s)
      Kuwasako K, Kitamura K, Nagata S, Hikosaka T, Kato J
    • Journal Title

      Peptides

      Volume: 32(7) Pages: 1540-1550

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Adrenomedullin production is increased in colorectal adenocarcinomas; its relation to matrix metalloproteinase-92011

    • Author(s)
      Hikosaka T, Tsuruda T, Nagata S, Kuwasako K, Tsuchiya K, Hoshiko S, Inatsu H, Chijiiwa K, Kitamura K
    • Journal Title

      Peptides

      Volume: 32(9) Pages: 1825-1831

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular basis of adrenomedullin 1 receptor function and its roles in the cardiovascular system2011

    • Author(s)
      Kuwasako K, Kitamura K, Nagata S, Kato J
    • Journal Title

      Curr Hypertens Rev

      Volume: 7 Pages: 207-216

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure-function analysis of helix 8 of human calcitonin receptor-like receptor within the adrenomedullin 1 receptor.2011

    • Author(s)
      Kuwasako, K.Kitamura, K.Nagata, S.Hikosaka, T.Kato, J.
    • Journal Title

      Peptides

      Volume: 32 Pages: 144-149

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Function of the cytoplasmic tail of human calcitonin receptor-like receptor in complex with receptor activity-modifying protein 22010

    • Author(s)
      Kuwasako K, Kitamura K, Nagata S, Hikosaka T, Kato J
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 392(3) Pages: 380-385

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plasma and tissue levels of proangiotensin-12 and components of the renin-angiotensin system (RAS) following low- or high-salt feeding in rats2010

    • Author(s)
      Nagata S, Kato J, Kuwasako K, Kitamura K
    • Journal Title

      Peptides

      Volume: 31(5) Pages: 889-892

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plasma and tissue levels of proangiotensin-12 and components of the renin-angiotensin system (RAS) following low- or high-salt feeding in rats.2010

    • Author(s)
      Nagata, S.Kato, J.Kuwasako, K.Kitamura, K.
    • Journal Title

      Peptides

      Volume: 31 Pages: 889-892

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] アンジオテンシン II 産生におけるプロアンジオテンシン-12 の役割2012

    • Author(s)
      永田さやか、桑迫健二、北村和雄
    • Organizer
      第85回 日本内分泌学会学術総会
    • Place of Presentation
      愛知
    • Related Report
      2012 Final Research Report
  • [Presentation] アンジオテンシンII産生におけるプロアンジオテンシンー12の役割2012

    • Author(s)
      永田さやか
    • Organizer
      日本内分泌学会総会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] G蛋白共役型受容体キナーゼ(GRK)の新作用:GRK4 と GRK5 による1型アドレノメデュリン受容体(AM 1 受容体)の細胞膜輸送と細胞内シグナリングの強力な抑制2011

    • Author(s)
      桑迫健二、北村和雄、永田さやか、野崎尚美、加藤丈司
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] Adrenomedullin production is increased in colorectal adenocaecinomas; its relation to matrix metalloproteinase-92011

    • Author(s)
      Hikosaka T, Tsuruda T, Nagata S, Ashizuka S, Inatsu H, Kitamura K
    • Organizer
      The 19th United European Gastroenterology Week
    • Place of Presentation
      Stockholm, Sweden
    • Related Report
      2012 Final Research Report
  • [Presentation] 2つのアドレノメデュリン(AM)受容体の活性化を規定する責任領域の特定と特徴化:CGRP 受容体と異なる新たな活性化機構2011

    • Author(s)
      桑迫健二、北村和雄、永田さやか、野崎尚美、加藤丈司
    • Organizer
      第34回日本高血圧学会総会
    • Place of Presentation
      宇都宮
    • Related Report
      2012 Final Research Report
  • [Presentation] 降圧系1型アドレノメデュリン(AM 1)受容体のへリックス8は AM1 受容体を負に制御し、Gs 蛋白の結合に不可欠である2011

    • Author(s)
      桑迫健二、北村和雄、永田さやか、彦坂ともみ、加藤丈司
    • Organizer
      第84回日本薬理学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] ラット骨芽細胞における増殖調節因子としてのニューロメディン B の生理的役割の解明.2011

    • Author(s)
      齊藤弘樹、井上和彦、池田龍二、中町友哉、塩田清二、永田さやか、北村和雄、南野直人、宮田篤郎
    • Organizer
      第84回日本薬理学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] プロアンジオテンシン-12 の単離同定と産生調節機序の検索2011

    • Author(s)
      加藤丈司、永田さやか、桑迫健二、北村和雄
    • Organizer
      第40回日本心脈管作動物質学会シンポジウム「RAAS ホットトピック」
    • Place of Presentation
      高松
    • Related Report
      2012 Final Research Report
  • [Presentation] Interaction between receptor activity-modifying proteins (RAMPs) and G-protein-coupled receptors and the function of the C-terminal tail of calcitonin receptor-like receptor in complex with RAMP2.2010

    • Author(s)
      Kuwasako, K.Kitamura, K.Nagata, S.Hikosaka, T.Kato, J.
    • Organizer
      7^<th> International Symposia on the CGRP Family ; CGRP, Adrenomedullin, Amylin, Intermedin and Calcitonin
    • Place of Presentation
      Queenstown, New Zealand
    • Year and Date
      2010-08-31
    • Related Report
      2010 Annual Research Report
  • [Presentation] Effect of adrenomedullin administration in two rat models of experimental inflammatory colitis.2010

    • Author(s)
      Nagata, S.Hikosaka, T.Kuwasako, K.Ashizuka, S.Tsuruda, T.Kato, J.Kitamura, K.
    • Organizer
      7^<th> International Symposia on the CGRP Family ; CGRP, Adrenomedullin, Amylin, Intermedin and Calcitonin
    • Place of Presentation
      Queenstown, New Zealand
    • Year and Date
      2010-08-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Increased levels of adrenomedullin protein and expression of related genes in local colorectal cancer.2010

    • Author(s)
      Hikosaka, T.Nagata, S.Tsuruda, T.Kuwasako, K.Inatsu ; H.Kitamura, K.
    • Organizer
      7^<th> International Symposia on the CGRP Family ; CGRP, Adrenomedullin, Amylin, Intermedin and Calcitonin
    • Place of Presentation
      Queenstown, New Zealand
    • Year and Date
      2010-08-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] G蛋白共役型受容体キナーゼ4(GRK4)と GRK5 によるアドレノメデュリン(AM)受容体の細胞膜輸送の強力な抑制効果およびその受容体上の責任領域の同定2010

    • Author(s)
      桑迫健二、北村和雄、永田さやか、加藤丈司
    • Organizer
      第33回日本高血圧学会総会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] Increased levels of adrenomedullin peptide and expression of related genes in local colorectal cancer.2010

    • Author(s)
      Hikosaka T,Nagata S, Thuruda T, Kuwasako K, Inatsu H, Kitamura K
    • Organizer
      The 7th International Symposia on the CGRP Family; CGRP, Adrenomedullin, Amylin, Intermedin and Calcitonin
    • Place of Presentation
      Queenstown, New Zealand
    • Related Report
      2012 Final Research Report
  • [Presentation] Interaction between receptor activity-modifying proteins (RAMPs) and G-protein-coupled receptors and the function of the C-terminal tail of calcitonin receptor-like receptor in complex with RAMP22010

    • Author(s)
      Kuwasako K,Kitamura K, Nagata S, Hikosaka T,Kato J
    • Organizer
      The 7th International Symposia on the CGRP Family; CGRP, adrenomedullin, Amylin, Intermedin and Calcitonin
    • Place of Presentation
      Queenstown, New Zealand
    • Related Report
      2012 Final Research Report
  • [Presentation] Effect of adrenomedullin administration in two rat models of experimental inflammatory colitis2010

    • Author(s)
      Nagata S, Hikosaka T, Ashizuka S, Tsuruda T, Kuwasako K, Kato J, Kitamura K
    • Organizer
      7th International Symposia on the CGRP Family; CGRP, Adrenomedullin, Amylin, Intermedin and Calcitonin
    • Place of Presentation
      Queenstown, New Zealand
    • Related Report
      2012 Final Research Report
  • [Presentation] Effects of salt depletion and RAS blockade on plasma and tissue levels of rat angiotensin-related peptides2010

    • Author(s)
      Nagata S, Kato J, Kuwasako K, Kitamura K
    • Organizer
      The 14th Scientific Session of the Society of Cardiovascular Endocrinology and Metabolism
    • Place of Presentation
      Nara, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] 1回膜貫通型の受容体活性調節蛋白が作用するG蛋白共役型受容体を同定するための簡易スクリーニング法の確立および応用解析2010

    • Author(s)
      桑迫健二、北村和雄、永田さやか、加藤丈司
    • Organizer
      第83回日本薬理学会年会
    • Place of Presentation
      大阪
    • Related Report
      2012 Final Research Report
  • [Presentation] Characterization of the trafficking interactions between endocrine G protein-coupled receptors and receptor activity-modifying proteins.2010

    • Author(s)
      Kuwasako K, Kitamura K, Nagata S, Kato J
    • Organizer
      The 14th International Congress of Endocrinology (ICE2010)
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2012 Final Research Report
  • [Remarks] 研究会 (1)永田さやか、北村和雄:循環器・腎臓疾患の新しいバイオマーカーの開発を目指して.第2回 ペプチド・ホルモン若手研究会、2012 年 3 月、広島

    • Related Report
      2012 Final Research Report
  • [Remarks] (2)永田さやか、加藤丈司、朝見麻希、北村和雄:ラット血中・組織中のアンジオテンシン II(Ang II)産生におけるプロアンジオテンシン-12(proang-12)の役割.第2回 Molecular Cardiovascular Conference、2011 年 9 月、北海道

    • Related Report
      2012 Final Research Report
  • [Remarks] (3)Hikosaka T, Nagata S, Ashizuka S, Inatsu H, Kitamura K: Therapeutic effect of adrenomedullin administration in two rat models of experimental inflammatory colitis. 第2回 Molecular Cardiovascular Conference、2011 年 9 月、北海道

    • Related Report
      2012 Final Research Report
  • [Remarks] (4)永田さやか、加藤丈司、桑迫健二、北村和雄:低塩刺激および RA 系阻害薬投与に伴うプロアンジオテンシン-12(proang-12)やレニン・アンジオテンシン系(RA 系)因子の血中・組織中の変化.第15回アンジオテンシンカンファレンス、2010 年 2 月、大阪

    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 新規ペプチドおよびその用途2012

    • Inventor(s)
      永田さやか、北村和雄、浅田裕士郎
    • Industrial Property Rights Holder
      宮崎大学
    • Industrial Property Number
      2012-244752
    • Filing Date
      2012-11-06
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 新規ペプチドおよびその用途2012

    • Inventor(s)
      永田さやか、北村和雄、浅田祐士朗
    • Industrial Property Rights Holder
      宮崎大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-11-06
    • Related Report
      2012 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi