Physiological roles of(pro) renin receptor in vascular smooth muscle cells

• Project/Area Number: 22790729
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Circulatory organs internal medicine
• Research Institution: Keio University
• Principal Investigator: MITO Asako 慶應義塾大学, 医学部, 助教 (20468474)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: (プロ)レニン受容体 / 血管平滑筋細胞 / 線維化 / V-ATPase / 繊維化

Research Abstract

This study is performed to examine the roles of(pro) renin receptor in the development and physiology of vascular smooth muscle cells. The smooth muscle cell-specific Atp6ap2 conditional knockout(CKO) mice were generated by mating female Atp6ap2-floxed mice with male mice that expressed the Cre recombinase under the control of the myosin heavy chain(Myh-11) promoter. Histological examination revealed that the CKO mice exhibited the decreased number of vascular smooth muscle cells and increased number of fibroblasts in the aorta due to vascular muscle cell death. Our researches revealed that the(P) RR/ATP6AP2 physiologically plays an essential role in the cell survival of mouse vascular smooth muscle cells through contributing to assembly and function of vacuolar H^+-ATPase.

Research Products

• [Presentation] 培養血管平滑筋細胞における(プロ)レニン受容体の生理学的役割 (2011)
  • Author(s): 三戸麻子
  • Organizer: 日本高血圧学会
  • Place of Presentation: 宇都宮東武ホテルグランデ
  • Year and Date: 2011-10-22
• [Presentation] 培養血管平滑筋細胞における(プロ)レニン受容体の生理学的役割 (2011)
  • Author(s): 三戸麻子
  • Organizer: 日本高血圧学会
  • Place of Presentation: 宇都宮東武ホテルグランデ(栃木)
  • Year and Date: 2011-10-22
• [Presentation] Physiological roles of(pro) renin receptor/ATP6AP2 in vascular smooth muscle cells (2011)
  • Author(s): Asako Mito
  • Organizer: American Heart Association High Blood Pressure counsil
  • Place of Presentation: Orlando, Florida, USA
  • Year and Date: 2011-09-21
• [Presentation] Physiological roles of (pro)renin receptor/ATP6AP2 in vascular smooth muscle cells (2011)
  • Author(s): Asako mito
  • Organizer: American Heart Association High Blood Pressure counsil
  • Place of Presentation: Orlando, Florida, USA
  • Year and Date: 2011-09-21
• [Presentation] 血管平滑筋細胞内リサイクルシステムにおける(プロ)レニン受容体の役割 (2011)
  • Author(s): 三戸麻子
  • Organizer: 日本腎臓学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-06-17
• [Presentation] 血管平滑筋細胞内リサイクルシステムにおける(プロ)レニン受容体の役割 (2011)
  • Author(s): 三戸麻子
  • Organizer: 日本腎臓学会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-06-17
• [Presentation] 平滑筋細胞特異的(プロ)レニン受容体ノックアウトマウスによるオートファジー障害 (2011)
  • Author(s): 三戸麻子
  • Organizer: 日本内分泌学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2011-04-21
• [Presentation] 平滑筋細胞特異的(プロ)レニン受容体ノックアウトマウスによるオートファジー障害 (2011)
  • Author(s): 三戸麻子
  • Organizer: 日本内分泌学会
  • Place of Presentation: 神戸国際会議場(兵庫)
  • Year and Date: 2011-04-21
• [Presentation] Physiological roles of(pro) renin receptor/ATP6AP2 in vascular smooth muscle cells (2011)
  • Author(s): Asako Kurauchi-Mito
  • Organizer: World congress of nephrology 2011
  • Place of Presentation: Vancouver Canada
  • Year and Date: 2011-04-10
• [Presentation] Physiological roles of (pro)renin receptor/ATP6AP2 in vascular smooth muscle cells (2011)
  • Author(s): Asako Kurauchi-Mito
  • Organizer: World congress of nephrology 2011
  • Place of Presentation: Vancouver Canada
  • Year and Date: 2011-04-10
• [Presentation] 平滑筋特異的(プロ)レニン受容体ノックアウトマウスの解析 (2011)
  • Author(s): 三戸麻子
  • Organizer: 日本心脈管作動物質学会
  • Place of Presentation: かがわ国際会議場
  • Year and Date: 2011-02-05