Analysis of Nucleotide sugar: Association with pathogenesis of COPD in Leukocyte

• Project/Area Number: 22790755
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: The Institute of Physical and Chemical Research (2011-2012); Osaka University (2010)
• Principal Investigator: TAKAMIYA Rina 独立行政法人理化学研究所, 糖鎖認識研究チーム, 基幹研究所研究員 (70365419)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 抗炎症性脂質メディエーター / 慢性閉塞性肺疾患 / マクロファージ / 活性酸素種 / 脂質メディエーター / COPD / SOD1 / 酸化ストレス / 糖鎖 / マクロフィージ / SOD-1

Research Abstract

It is well known that cigarette smoke (CS) induces disability of macrophage function by oxidative stress. We indicated that CS induced bisecting GlcNAc modification in macrophage. Furthermore, pretreatment of macrophages with a pro-resolving mediator, Resolvin E1(RvE1) restored the phagocytic activity and reduced cell death induced by treatment of CSE. These results suggest that RvE1 have new potential therapeutic applications in COPD.

Research Products

• [Journal Article] The interaction between Siglec-15 and tumor-associated sialyl-Tn antigen enhances TGF-β secretion from monocytes/macrophages through the DAP12-Syk pathway (2013)
  • Author(s): Takamiya R, Ohtsubo K, Takamatsu S, Taniguchi N, Angata T
  • Journal Title: Glycobiology Volume: 23 Pages: 178-187
  • Peer Reviewed
• [Journal Article] The interaction between Siglec-15 and tumor-associated sialyl-Tn antigen enhances TGF-β secretion from monocytes/macrophages through the DAP12-Syk pathway. (2013)
  • Author(s): Rina Takamiya
  • Journal Title: Glycobiology Volume: 23 Issue: 2 Pages: 178-187
  • DOI: 10.1093/glycob/cws139
  • Peer Reviewed
• [Journal Article] Dysregulated synthesis of protectin D1 in eosinophils from patients with severe asthma (2013)
  • Author(s): Miyata J, Fukunaga K, Iwamoto R, Isobe Y, Niimi K, Takamiya R, Takihara T, Tomomatsu K, Suzuki Y, Oguma T, Sayama K, Arai H, Betsuyaku T, Arita M, Asano K.
  • Journal Title: J Allergy Clin Immunol Volume: 131 Issue: 2 Pages: 353
  • DOI: 10.1016/j.jaci.2012.07.048
  • Peer Reviewed
• [Journal Article] Resolvin E1 maintains macrophage function under cigarette smoke-induced oxidative stress (2012)
  • Author(s): Takamiya R, Fukunaga K, Arita M, Miyata J, Seki H, Minematsu N, Suematsu M, Asano K
  • Journal Title: FEBS Open Bio Volume: 2 Pages: 328-333
  • Peer Reviewed
• [Journal Article] Dysregulated synthesis of protectin D1 in eosinophils from patients with severe asthma (2012)
  • Author(s): Miyata J, Fukunaga K, Iwamoto R, Isobe Y, Niimi K, Takamiya R, Takihara T, Tomomatsu K, Suzuki Y, Ogum, Sayama K, Arai H, Betsuyaku T, Arita M, Asano K
  • Journal Title: J Allergy Clin Immun
  • Peer Reviewed
• [Journal Article] Annexin V decreases PS-1 mediated macrophage efferocytosis and deteriorates elastase-induced pulmonary emphysema in mice (2012)
  • Author(s): Yoshida S, Minematsu N, Chubachi S, Nakamura H, Miyazaki M, Tsuduki K, Takahashi S, Miyasho T, Iwabuchi T, Takamiya R, Tateno H, Mouded M, Shapiro SD, Asano K, Betsuyaku T
  • Journal Title: Am J Physiol Lung Cell Mol Physiol Volume: 303
  • Peer Reviewed
• [Journal Article] Annexin V decreases PS-mediated macrophage efferocytosis and deteriorates elastase-induced pulmonary emphysema in mice. (2012)
  • Author(s): Shuichi Yoshida
  • Journal Title: Am J Physiol Lung Cell Mol Physiol. Volume: 303 Issue: 10 Pages: L852-L860
  • DOI: 10.1152/ajplung.00066.2012
  • Peer Reviewed
• [Journal Article] Carbon black nanoparticles enhance bleomycin-induced lung inflammatory and fibrotic changes in mice (2011)
  • Author(s): Kamata H, Tasaka S, Inoue K, Miyamoto K, Nakano Y, Shinoda H, Kimizuka Y, Fujiwara H, Ishii M, Hasegawa N, Takamiya R, Fujishima S, Takano H, Ishizaka A,
  • Journal Title: Exp Biol Med (Maywood) Volume: 236 Pages: 315-324
  • Peer Reviewed
• [Presentation] 癌関連糖鎖抗原Sialyl-Tn によるがん微小環境調節機構の解明 (2012)
  • Author(s): 高宮里奈、大坪和明、高松真二、白土健、谷口直之、安形高志
  • Organizer: 第85回日本生化学会
  • Place of Presentation: 福岡
• [Presentation] Regulation of tumor microenvironment by tumor-associated sialyl-Tn antigen through ROS production by immune cells (2012)
  • Author(s): Takamiya R, Ohtsubo K, Takamatsu S, Taniguchi N, Angata T
  • Organizer: The 33rd Naito Conference on Oxygen Biology: Hypoxia, oxidative stress and disease
  • Place of Presentation: Sapporo
• [Presentation] 癌関連糖鎖抗原Sialyl-Tnによるがん微小環境調節機構の解明 (2012)
  • Author(s): 高宮里奈
  • Organizer: 第８５回日本生化学会
  • Place of Presentation: 福岡
• [Presentation] Regulation of tumor microenvironment by tumor-associated sialyl-Tn antigen through ROS production by immune cells (2012)
  • Author(s): Rina Takamiya
  • Organizer: The 33rd Naito Conference on Oxygen Biology: Hypoxia, oxidative stress and disease
  • Place of Presentation: 札幌
• [Presentation] 癌特異的糖鎖抗原Sialyl-Tnによる活性酸素種産生を伴う単球の活性化機構の解明 (2011)
  • Author(s): 高宮里奈、大坪和明、高松真二、白土健、谷口直之、安形高志
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] Sialyl-Tn抗原の認識による活性酸素種産生を伴う単球細胞株の活性化 (2011)
  • Author(s): 高宮里奈、大坪和明、高松真二、白土健、谷口直之
  • Organizer: 第64回酸化ストレス学会
  • Place of Presentation: 留寿都
  • Year and Date: 2011-07-03
• [Presentation] 癌特異的糖鎖抗原Sialyl-Tn による活性酸素種産生を伴う単球の活性化機構の解明 (2011)
  • Author(s): 高宮里奈、大坪和明、高松真二、白土健、谷口直之、安形高志
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都
• [Presentation] Regulation of monocyte function by tumor associated sialyl-Tn antigen through ROS production (2011)
  • Author(s): Takamiya R, Ohtsubo K, Takamatsu S, Taniguchi N, Angata T
  • Organizer: 5th SFRR-ASIA, 8th ASMRM, 11th J-mit 2011
• [Presentation] Sialyl-Tn 抗原の認識による活性酸素種産生を伴う単球細胞株の活性化 (2011)
  • Author(s): 高宮里奈、大坪和明、高松真二、白土健、谷口直之、安形高志
  • Organizer: 第64回酸化ストレス学会
  • Place of Presentation: 留寿都
• [Presentation] Cigarette smoke extract induces bisecting GlcNAc modification in RAW264.7 murine macrophage (2010)
  • Author(s): 高宮里奈
  • Organizer: 7th International Symposium on Glycosyltransferases
  • Place of Presentation: 東京
  • Year and Date: 2010-07-31
• [Presentation] High mobility group A1 protein mediates human nitric oxide synthase 2 gene expression (2010)
  • Author(s): 高宮里奈
  • Organizer: The 6th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
  • Place of Presentation: 京都
  • Year and Date: 2010-06-17
• [Presentation] Baron RM, Perrella MA, A deficiency of heme oxygenase-1 leads to increased baseline inflammation and enhanced expression of HMGB1 in macrophages (2010)
  • Author(s): 高宮里奈、永石宇司、Owen C, Hall SR, Ith B, 安形高志、谷口直之、前野敏孝
  • Organizer: 日本生化学会・分子生物学会合同大会
  • Place of Presentation: 神戸
• [Presentation] Cigarette smoke extract induces bisecting GlcNAc modification in RAW264.7 murine macrophage (2010)
  • Author(s): Takamiya R, Ohtsubo K, Takamatsu S, Nakajima K, Shirato K, Taniguchi N, Angata T
  • Organizer: 7th International Symposium on Glycosyltransferases
  • Place of Presentation: Tokyo
• [Presentation] High mobility group A1 protein mediates human nitric oxide synthase 2 gene expression (2010)
  • Author(s): Takamiya R, Baron RM, Yet S-F, Layne MD, Angata T, Taniguchi N, Perrella MA
  • Organizer: The 6th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
  • Place of Presentation: Kyoto
• [Presentation] A deficiency of heme oxygenase-1 leads to increased baseline inflammation and enhanced expression of HMGB1 in macrophages (2010)
  • Author(s): 高宮里奈
  • Organizer: BMB2010
  • Place of Presentation: 神戸