Disordered expression of the homing receptor glycans on helper memory T cells in patients with severe bronchial asthma

• Project/Area Number: 22790774
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: SAKUMA Keiichiro 愛知県がんセンター(研究所), 分子病態学部, 主任研究員 (90402891)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 閉塞性肺疾患 / 喘息 / ホーミング / メモリーT細胞 / 糖鎖 / ヘルパーメモリーT細胞 / 硫酸基転移酵素 / 転写 / アトピー性皮膚炎

Research Abstract

1.The expression levels of the sulfated homing-receptor glycan on helper T(Th) cells were higher in patients with bronchial asthma or atopic dermatitis than those in normal controls. These levels were significantly correlated with frequency of exacerbation of atopic dermatitis, while the expression levels of non-sulfated Th-trafficking glycan were correlated with the disease severity at the time when the blood sample was collected. Statistic analysis suggested potential utility of the sulfated homing-receptor glycan for discriminating patients with highly recurrent atopic dermatitis.
2.We have reported the mechanism of the transcriptional regulation of a sulfotransferase gene HEC-GlcNAc6ST. We have also revealed the involvement of HEC-GlcNAc6ST in the synthesis of a sulfated cell-adhesion glycan in helper T cells.

Research Products

• [Journal Article] Colonic epithelial cells express specific ligands for mucosal macrophage immunosuppressive receptors, siglec-7 and-9 (2012)
  • Author(s): Miyazaki, K., Sakuma, K., Kawamura, Y. I., Izawa, M., Ohmori, K., Mitsuki, M., Yamaji, T., Hashimoto, Y., Suzuki, A., Saito, Y., Dohi, T., and Kannagi, R.
  • Journal Title: J. Immunol Volume: 188 Issue: 9 Pages: 4690-4700
  • DOI: 10.4049/jimmunol.1100605
  • Peer Reviewed
• [Journal Article] Induction of 6-sulfated glycans with cell adhesion activity via T-bet and GATA-3 in human helper T cells (2012)
  • Author(s): Sakuma K, Chen GY, Aoki M, Kannagi R.
  • Journal Title: Biochim Biophys Acta Volume: 1820 Issue: 7 Pages: 841-848
  • DOI: 10.1016/j.bbagen.2012.03.005
  • Peer Reviewed
• [Journal Article] Transcription factors c-MYC and CDX2 mediate E-selectin ligand expression in colon cancer cells undergoing EGF/bFGF-induced epithelial-mesenchymal transition (2012)
  • Author(s): Sakuma K, Aoki M, Kannagi R.
  • Journal Title: Proc Natl Acad Sci USA Volume: 109(20) Issue: 20 Pages: 7776-7781
  • DOI: 10.1073/pnas.1111135109
  • Peer Reviewed
• [Journal Article] Sialylated and sulfated carbohydrate ligands for selectins and siglecs : involvement in traffic and homing of human memory T and B lymphocytes (2011)
  • Author(s): Kannagi, R., Ohmori, K., Chen, G. Y., Miyazaki, K., Izawa, M., and Sakuma, K.
  • Journal Title: Adv. Exp. Med. Biol Volume: 705 Pages: 549-569
  • DOI: 10.1007/978-1-4419-7877-6_29
  • ISBN: 9781441978769, 9781441978776
  • Peer Reviewed
• [Presentation] Th2-specific homing ligands involved in recurrent atopic dermatitis exacerbations (2011)
  • Author(s): 佐久間圭一朗
  • Organizer: 日本免疫学会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2011-11-27
• [Presentation] EGF and bFGF induce EMT and E-selectin ligandglycan expression on colorectal cancer cells (2011)
  • Author(s): 佐久間圭一朗、青木正博、神奈木玲児
  • Organizer: 第70回日本癌学会総会
  • Place of Presentation: 名古屋
• [Presentation] Th2-specific homing ligands involved in recurrent atopic dermatitis exacerbations. (2011)
  • Author(s): 佐久間圭一朗、古橋卓也、近藤幸子、矢部宇一郎、大森勝之、森田明理、神奈木玲児
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 千葉
• [Presentation] Quantifying carbohydrate adhesion molecules on helper memory T cells for the diagnosis and evaluation of atopic dermatitis severity (2010)
  • Author(s): 古橋卓也
  • Organizer: 日本研究皮膚科学会
  • Place of Presentation: 和歌山県民文化会館(和歌山県)
  • Year and Date: 2010-12-04
• [Remarks]
  • URL: http://www.pref.aichi.jp/cancer-center/ri/01bumon/07bunshi_byotai/index.html
• [Remarks]
  • URL: http://www.pref.aichi.jp/cancer-center/ri/01bumon/07bunshi_byotai/index.html
• [Remarks]
  • URL: http://www.pref.aichi.jp/cancer-center/400/420/421/421-07.html