Research for novel regulatory mechanisms by functional analysis of novel bioactive peptide and its receptor

• Project/Area Number: 22790892
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: MORI Kenji 独立行政法人国立循環器病研究センター, 生化学部, 室長 (00416223)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 内分泌 / 神経ペプチド / 生理活性ペプチド / 受容体

Research Abstract

A novel bioactive peptide was identified by in silico screening. This peptide was produced from a precursor protein of known neuropeptide by differential proteolytic processing. Intracerebroventricular administration of peptide to rats induced increase in plasma concentration of specific pituitary hormone. A specific binding of radiolabeled peptide to a section of rat brain was observed. To elucidate the physiological role of a newly identified peptide, mice lacking only this peptide were generated.

Research Products

• [Journal Article] Identification of the novel bioactive peptides dRYamide-1 and dRYamide-2, ligands for a neuropeptide Y-like receptor in Drosophila (2011)
  • Author(s): Ida T, Takahashi T, Tominaga H, Sato T, Kume K, Ozak Mi, Hiraguchi T, Maeda T, Shiotani H, Terajima S, Sano H, Mori K, Yoshida M, Miyazato M, Kato J, Murakami N, Kangawa K, Kojima M
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Issue: 4 Pages: 872-877
  • DOI: 10.1016/j.bbrc.2011.06.081
  • Peer Reviewed
• [Journal Article] ニューロメジンＳとその多様な機能 (2011)
  • Author(s): 森健二、森美和、宮里幹也、寒川賢治
  • Journal Title: Comparative Endocrinology Volume: 37 Issue: 142 Pages: 124-133
  • DOI: 10.5983/nl2008jsce.37.124
  • NAID: 130004509341
  • ISSN: 1882-6636, 1882-6644
• [Journal Article] ニューロメジンUノックアウトマウス (2011)
  • Author(s): 森健二、児島将康
  • Journal Title: The Lipid Volume: 第22巻 Pages: 4-9
• [Journal Article] 代謝制御と日内リズムをリンクするニューロメジンUとニューロメジンS (2011)
  • Author(s): 森健二、宮里幹也、児島将康、寒川賢治
  • Journal Title: 実験医学増刊"代謝・内分泌ネットワークと医薬応用" Volume: 第29巻 Pages: 60-66
• [Journal Article] Neuromedin S regulates cardiovascular function through the sympathetic nervous system in mice (2011)
  • Author(s): Sakamoto T, Nakahara K, Maruyama K, Katayama T, Mori K, Miyazato M, Kangawa K, Murakami N
  • Journal Title: Peptides Volume: 32 Issue: 5 Pages: 1020-1026
  • DOI: 10.1016/j.peptides.2011.02.015
  • Peer Reviewed
• [Journal Article] ニューロメジンUとニューロメジンS (2011)
  • Author(s): 森健二、寒川賢治、児島将康
  • Journal Title: 生体の科学 Volume: 第62巻 Pages: 51-56
• [Journal Article] ニューロメジンSとその多様な機能 (2011)
  • Author(s): 森健二, 他
  • Journal Title: 比較内分泌 Volume: 37 Pages: 124-133
  • NAID: 10029335405
• [Journal Article] ニューロメジンUノックアウトマウス (2011)
  • Author(s): 森健二, 他
  • Journal Title: The Lipid Volume: 22 Pages: 4-9
• [Journal Article] 代謝制御と日内リズムをリンクするニューロメジンUとニューロメジンS (2011)
  • Author(s): 森健二, 他
  • Journal Title: 実験医学増刊"代謝・内分泌ネットワークと医薬応用" Volume: 29 Pages: 60-66
• [Journal Article] Neuromedin S regulates cardiovascular function through the sympathetic nervous system in mice. (2011)
  • Author(s): Sakamoto T, et al.
  • Journal Title: Peptides Volume: 32 Pages: 1020-1026
  • Peer Reviewed
• [Journal Article] ニューロメジンUとニューロメジンS (2011)
  • Author(s): 森健二, 他
  • Journal Title: 生体の科学 Volume: 62 Pages: 51-56
• [Journal Article] Comparison of feeding suppression by the anorexigenic hormones neuromedin U and neuromedin S in rats (2010)
  • Author(s): Nakahara K, Katayama T, Maruyama K, Ida T, Mori K, Miyazato M, Kangawa K, Murakami N
  • Journal Title: J Endocrinol Volume: 207 Issue: 2 Pages: 185-193
  • DOI: 10.1677/joe-10-0081
  • Peer Reviewed
• [Journal Article] Comparison of feeding suppression by the anorexigenic hormones neuromedin U and neuromedin S in rats. (2010)
  • Author(s): Nakahara K, et al.
  • Journal Title: Journal of Endocrinology Volume: 207 Pages: 185-193
  • Peer Reviewed
• [Presentation] 摂食調節におけるニューロメジンSとニューロメジンU (2011)
  • Author(s): 森健二、宮里幹也、中原桂子、村上昇、寒川賢治
  • Organizer: 第16回アディポサイエンス研究会
  • Place of Presentation: 大阪(千里)
  • Year and Date: 2011-08-20
• [Presentation] Purification of a peptide from rat brain (2010)
  • Author(s): Mori K, Miyazato M, Kangawa K
  • Organizer: 5th International Peptide Symposium
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-12-06
• [Presentation] Purification of a peptide from rat brain. (2010)
  • Author(s): Mori K, et al.
  • Organizer: 5th International Peptide Symposium
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-12-06