| Project/Area Number |
22790898
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Chiba University |
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Principal Investigator |
OSAWA Mitsujiro 千葉大学, 大学院・医学研究院, 特任講師 (70546770)
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 血液内科学 / 再生医学 / ヒトES細胞 / ヒトiPS細胞 / 造血幹細胞 / TGFβ阻害剤 / Sox17遺伝子 / 一酸化窒素供与体 / Activin A / HoxB4 / Sox17 |
|---|
| Research Abstract |
To understand the molecular basis of hematopoietic differentiation from human induced pluripotent stem cells (hiPSCs), we first performed chemical compound screening using in vitro differentiation system based on embryoid body (EB) formation. From this screen, we found that several TGFβ inhibitors enhanced hematopoietic cell differentiation. As the result, we established an efficient methodfor induction of hematopoietic stem and progenitor cells from hiPSCs. We also evaluated the effects of overexpression of key molecules predicted to support the generation and maturation of HSCs. Among genes tested, we found that SOX17 plays a critical role in priming hemogenic potential of endothelial cells, thereby regulating the hematopoietic development from hiPSCs.
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