Single cell Analysis of asymmetrical cell division of hematopoietic stem cell

• Project/Area Number: 22790920
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: Keio University
• Principal Investigator: HOSOKAWA Kentaro 慶應義塾大学, 医学部, 特任助教 (90569584)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 造血幹細胞 / 非対称分裂 / 単一細胞 / Tie2 / Paired daughter cells / 体外増幅 / Angiopoietin-1 / single cell / 遺伝子 / 移植・再生医療 / 細胞・組織 / 再生医学 / 蛋白質 / 発生・分化

Research Abstract

We focused on the distribution of Tie2 on the Pired Daughter Cells(PDCs) of hematopoietic stem cells from the gene expression analysis data. The distribution pattern of Tie2 to PDCs was asymmetrically on steady state, on the other hand, it was symmetrically on the proliferative condition. Moreover, it was suggested that stimulation of Tie2 ligand, Ang-1 shifted the cell division pattern to asymmetric. It will be expected that the operation of cell division pattern of hematopoietic stem cells and stem cell expansion from this research.

Research Products

• [Journal Article] p57(Kip2) and p27(Kip1) cooperate to maintain hematopoietic stem cell quiescence through interactions with Hsc70 (2011)
  • Author(s): Zou P, Yoshihara H, Hosokawa K, Tai I, Shinmyozu K, Tsukahara F, Maru Y, Nakayama K, Nakayama KI, Suda T
  • Journal Title: Cell Stem Cell Volume: 9(3) Pages: 247-261
  • Peer Reviewed
• [Journal Article] Telomerase reverse transcriptase protects ATM-deficient hematopoietic stem cells from ROS-induced apoptosis through a telomere-independent mechanism (2011)
  • Author(s): Nitta E, Yamashita M, Hosokawa K, Xian M, Takubo K, Arai F, Nakada S, Suda T
  • Journal Title: Blood Volume: 117(16) Pages: 4169-4180
  • Peer Reviewed
• [Journal Article] p57^<Kip2> and p27^<Kip1> cooperate to maintain hematopoietic stem cell quiescence through interaction with Hsc70 (2011)
  • Author(s): Zou P, et al
  • Journal Title: Cell Stem Cell Volume: 9 Issue: 3 Pages: 247-261
  • DOI: 10.1016/j.stem.2011.07.003
  • Peer Reviewed
• [Journal Article] Telomerase reverse transcriptase protects ATM-deficient hematopoietic stem cells from ROS-induced apoptosis through a telomere independent mechanism (2011)
  • Author(s): Nitta Eriko
  • Journal Title: Blood Volume: 117 Issue: 16 Pages: 4169-80
  • DOI: 10.1182/blood-2010-08-297390
  • Peer Reviewed
• [Journal Article] Knockdown of N-cadherin suppresses the long-term engraftment of hematopoietic stem cells. (2011)
  • Author(s): Hosokawa K., et al.
  • Journal Title: Blood Volume: 116 Pages: 554-563
  • Peer Reviewed
• [Journal Article] Isolation and characterization of endosteal niche cell populations that regulate hematopoietic stem cells (2010)
  • Author(s): Nakamura Y, Arai F, Iwasaki H, Hosokawa K, Kobayashi I, Gomei Y, Matsumoto Y, Yoshihara H, Suda T
  • Journal Title: Blood Volume: 116(9) Pages: 1422-1432
  • Peer Reviewed
• [Journal Article] Knockdown of N-cadherin suppresses the long-term engraftment of hematopoietic stem cells (2010)
  • Author(s): Hosokawa K, Arai F, Yoshihara H, Iwasaki H, Nakamura Y, Gomei Y, Suda T
  • Journal Title: Blood Volume: 116(4) Pages: 554-563
  • Peer Reviewed
• [Journal Article] Cadherin-based adhesion is a potential target for niche manipulation to protect hematopoietic stem cells in adult bone marrow (2010)
  • Author(s): Hosokawa K, Arai F, Yoshihara H, Iwasaki H, Hembree M, Yin T, Nakamura Y, Gomei Y, Takubo K, Shiama H, Matsuoka S, Li L, Suda T
  • Journal Title: Cell Stem Cell Volume: 6(3) Pages: 194-198
  • Peer Reviewed
• [Journal Article] Functional differences between two Tie2 ligands, angiopoietin-1 and-2, in regulation of adult bone marrow hematopoietic stem cells (2010)
  • Author(s): Gomei Y, Nakamura Y, Yoshihara H, Hosokawa K, Iwasaki H, Suda T, Arai F
  • Journal Title: Exp Hematol Volume: 38 Pages: 82-89
  • Peer Reviewed
• [Journal Article] Isolation and characterization of endosteal niche cell populations that regulate hematopoietic stem cells. (2010)
  • Author(s): Nakamura Y., et al.
  • Journal Title: Blood Volume: 116 Pages: 1422-1432
  • Peer Reviewed
• [Presentation] Function of Protection of Telomere (POT1) in the maintenance of hematopoietic stem cells (2011)
  • Author(s): 細川健太郎
  • Organizer: KEY Forum in Developmental Biology and Regenerative Medicine
  • Place of Presentation: 熊本大学・熊本
  • Year and Date: 2011-09-09
• [Presentation] Function of Protection of Telomere(POT1) in the maintenance of hematopoietic stem cells (2011)
  • Author(s): Hosokawa K, Arai F, Nojima Y and Suda T
  • Organizer: KEY Forum in Developmental Biology and Regenerative Medicine
  • Place of Presentation: Kumamoto(Japan)
• [Presentation] Protection of Telomere (Pot1)による造血幹細胞の静止期状態維持機構の解析 (2010)
  • Author(s): 細川健太郎
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜市、横浜
  • Year and Date: 2010-09-24
• [Presentation] FUNCTION OF PROTECTION OF TELOMERE (POT1) IN THE MAINTENANCE OF HEMATOPOIETIC STEM CELLS. (2010)
  • Author(s): 細川健太郎
  • Organizer: ISEH 40th Annual Scientific Meeting
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2010-08-17
• [Presentation] Function of Protection of Telomere(Pot1) in the maintenance of hematopoietic stem cells (2010)
  • Author(s): Hosokawa K, Arai F, Nojima Y and Suda T
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜(神奈川)
• [Presentation] FUNCTION OF PROTECTION OF TELOMERE(POT1) IN THE MAINTENANCE OF HEMATOPOIETIC STEM CELLS (2010)
  • Author(s): Hosokawa K, Arai F, Nojima Y and Suda T
  • Organizer: ISEH 2010 Meeting
  • Place of Presentation: Melbourne(Australia)
• [Remarks]
  • URL: http://web.sc.itc.keio.ac.jp/celldiff/index.html