Analysis of wheat protein in sera from patients with wheat allergy using mass spectrometer

• Project/Area Number: 22790930
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Shimane University
• Principal Investigator: TAKAHASHI Hitoshi 島根大学, 医学部, 助教 (10432618)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 食物アレルギー / 抗原 / IgE / 質量分析

Research Abstract

The aims of this study were to identify wheat proteins in sera of patient with wheat dependent exercise-induced anaphylaxis (WDEIA) during provocation tests using iTRAQ-based analysis, and to evaluate usefulness of CAP-fluorescent enzyme-immunoassay (CAP-FEIA) using recombinant high molecular weight-glutein. In iTRAQ-based analysis, although wheat proteins were detected in patients’ sera, those wheat proteins were not involving with allergic reactions. When recombinant HMW-glutenin was immobilized to CAP-FEIA, it was confirmed that detection of specific IgE anaginst recombinant HMW-glutenin is useful for diagnosis of WDEIA when combined with the CAP-FEIA (recombinant omega-5 gliadin) test.

Research Products

• [Journal Article] Serum gliadin monitoring extracts patients with false negative results in challenge tests for the diagnosis of wheat-dependent exercise-induced anaphylaxis. (2013)
  • Author(s): Kohno K, Matsuo H, Takahashi H, Niihara H, Chinuki Y, Kaneko S, Honjoh T, Horikawa T, Mihara S, Morita E.
  • Journal Title: Allergol Int. Volume: 62 Pages: 229-38
  • NAID: 130004873070
  • Peer Reviewed
• [Journal Article] Higher allergenicity of high molecular weight hydrolysed wheat protein in cosmetics for percutaneous sensitization. (2013)
  • Author(s): Chinuki Y, Takahashi H, Dekio I, Kaneko S, Tokuda R, Nagao M, Fujisawa T, Morita E.
  • Journal Title: Contact Dermatitis. Volume: 68 Pages: 86-93
  • Peer Reviewed
• [Journal Article] Serum Gliadin Monitoring Extracts Patients with False Negative Results in Challenge Tests for the Diagnosis of Wheat-Dependent Exercise-Induced Anaphylaxis. (2013)
  • Author(s): Kohno K, Matsuo H, Takahashi H, Niihara H, Chinuki Y, Kaneko S, Honjoh T, Horikawa T, Mihara S, Morita E.
  • Journal Title: Allergol Int. Volume: 掲載確定
  • NAID: 130004873070
  • Peer Reviewed
• [Journal Article] CD203c expression-based basophil activation test for diagnosis of wheat-dependent exercise-induced anaphylaxis. (2012)
  • Author(s): Chinuki Y, Kaneko S, Dekio I, Takahashi H, Tokuda R, Nagao M, Fujisawa T, Morita E.
  • Journal Title: J Allergy Clin Immunol. Volume: 129 Pages: 1404-6
  • Peer Reviewed
• [Journal Article] Prevalence of wheat allergy in Japanese adults. (2012)
  • Author(s): Morita E, Chinuki Y, Takahashi H,Nabika T, Yamasaki M, Shiwaku K.
  • Journal Title: Allergol Int. Volume: 61 Pages: 101-5
  • NAID: 130004873026
  • Peer Reviewed
• [Journal Article] Recombinant high molecular weight-glutenin subunit-specificIgE detection is useful in identifying wheat-dependent exercise-induced anaphylaxis complementary to recombinant omega-5 gliadin-specific IgE test. (2012)
  • Author(s): Takahashi H, Matsuo H, Chinuki Y,Kohno K, Tanaka A, Maruyama N,Morita E.
  • Journal Title: Clin Exp Allergy. Volume: 42 Pages: 1293-8
  • Peer Reviewed
• [Journal Article] Recombinant high molecular weight-glutenin subunit-specific IgE detection is useful in identifying wheat-dependent exercise-induced anaphylaxis complementary to recombinant omega-5 gliadin-specific IgE test. (2012)
  • Author(s): Takahashi H, Matsuo H, Chinuki Y, Kohno K, Tanaka A, Maruyama N, Morita E.
  • Journal Title: Clin Exp Allergy. Volume: 42 Issue: 8 Pages: 1293-1298
  • DOI: 10.1111/j.1365-2222.2012.04039.x
  • Peer Reviewed
• [Journal Article] Prevalence of Wheat Allergy in Japanese Adults (2012)
  • Author(s): Morita E, Chinuki Y, Takahashi H, Nabika T, Yamasaki M, Shiwaku K.
  • Journal Title: Allergology International Volume: 61 Issue: 1 Pages: 101-105
  • DOI: 10.2332/allergolint.11-OA-0345
  • NAID: 130004873026
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed
• [Presentation] 小麦水溶性アレルゲンの大腸菌における可溶化発現. (2012)
  • Author(s): 松山梓、横大路智治、 高橋仁、森田栄伸、松尾裕彰.
  • Organizer: 日本皮膚科学会第124回山陰・第20回島根合同開催地方会
  • Place of Presentation: ホテルサンルート米子(米子市).
  • Year and Date: 2012-07-22
• [Presentation] リコンビナント小麦蛋白質特異 IgE 測定の有用性. (2012)
  • Author(s): 千貫祐子、松尾裕彰、新原邦江、 高橋仁、田中昭、森田栄伸.
  • Organizer: 第42回日本皮膚アレルギー・接触皮膚炎学会総会学術大会
  • Place of Presentation: 軽井沢プリンスホテルウエスト(軽井沢).
• [Presentation] 食物アレルギーとセツキシマブアレルギー. (2012)
  • Author(s): 高橋 仁、千貫祐子、森田栄伸.
  • Organizer: 第42回日本皮膚アレルギー・接触皮膚炎学会総会学術大会
  • Place of Presentation: 軽井沢プリンスホテルウエスト(軽井沢).
• [Presentation] 牛肉アレルギーの抗原の同定とセツキシマブとの交叉反応性. (2012)
  • Author(s): 高橋 仁、千貫祐子、森田栄伸.
  • Organizer: 第24回日本アレルギー学会春季臨床大会
  • Place of Presentation: 大阪国際会議場(大阪市).