Is a prion hypothesis the validity?: The real pathogen explored from the host innate immune systems

• Project/Area Number: 22790955
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Infectious disease medicine
• Research Institution: Nagasaki University
• Principal Investigator: ISHIBASHI Daisuke 長崎大学, 大学院・医歯薬学総合研究科, 助教 (10432973)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: プリオン / 自然免疫 / IRF3

Research Abstract

In this research assignment, we focused the innate immune system in host, and examined the relationship between prion infection and the system. As a result, we revealed that transcription factor IRF3 plays a key role in prion infection. Consequently, we considered that development of prevention and therapeutic agent against the prion disease which targeted the innate immune system would progress by this research project.

Research Products

• [Journal Article] Protective role of interferon regulatory factor3-mediated signaling against prion infection (2012)
  • Author(s): Ishibashi D, Atarashi R, Fuse T, Nakagaki T, Yamaguchi N, Satoh K, Honda K, Nishida N.
  • Journal Title: J Virol Volume: (In press)
  • Peer Reviewed
• [Journal Article] Direct evidence of generation and accumulation ofβ-sheet-rich prion protein in ScN2a cells de novo illuminated by human IgG1 antibody recognizingβ-form but notα-form of prion protein (2012)
  • Author(s): Kubota T, Hamazoe Y, Hashiguchi S, Ishibashi D, Akasaka K, Nishida N, Katamine S, Sakaguchi S, Kuroki R, Nakashima T, Sugimura K.
  • Journal Title: J Biol Chem Volume: (In press)
  • Peer Reviewed
• [Journal Article] Antigenic mimicry-mediated anti-prion effects induced by bacterial enzyme succinylarginine dihydrolase in mice (2011)
  • Author(s): Ishibashi D, Yamanaka H, Mori T, Yamaguchi N, Yamaguchi Y, Nishida N, Sakaguchi S.
  • Journal Title: Vaccine Volume: 29(50) Pages: 9321-8
  • Peer Reviewed
• [Journal Article] Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion (2011)
  • Author(s): Atarashi R, Satoh K, Sano K, Fuse T, Yamaguchi N, Ishibashi D, Matsubara T, Nakagaki T, Yamanaka H, Shirabe S, Yamada M, Mizusawa H, Kitamoto T, Klug G, McGlade A, Collins SJ, Nishida N.
  • Journal Title: Nat Med Volume: 17(2) Pages: 175-8
  • Peer Reviewed
• [Journal Article] Antigenic mimicry-mediated anti-prion effects induced by bacterial enzyme succinylarginine dihydrolase in mice (2011)
  • Author(s): Ishibashi D, Yamanaka H, Mori T, Yamaguchi N, Yamaguchi Y, Nishida N, Sakaguchi S
  • Journal Title: Vaccine Volume: 29(50) Issue: 50 Pages: 9321-9328
  • DOI: 10.1016/j.vaccine.2011.10.017
  • Peer Reviewed
• [Journal Article] Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion. (2011)
  • Author(s): Ryuichiro Atarashi, Katsuya Satoh, Kazunori Sano, Takayuki Fuse, Naohiro Yamaguchi, Daisuke Ishibashi, et al.
  • Journal Title: Nature medicine Volume: 17 Pages: 175-178
  • Peer Reviewed
• [Journal Article] 「プリオン仮説」の証明はどこまで進んだのか? Today, How much do we know now about the prion theory (2010)
  • Author(s): 石橋大輔
  • Journal Title: 最新医学 Volume: 65巻10号 Pages: 2306-2310
• [Journal Article] 「プリオン仮説」の証明はどこまで進んだのか? (2010)
  • Author(s): 石橋大輔
  • Journal Title: 最新医学 Volume: 65 Pages: 2306-2310
  • NAID: 40017344503
• [Presentation] 異常型プリオン蛋白の細胞内代謝機構の解明 (2012)
  • Author(s): 本間拓二郎、石橋大輔、西田教行
  • Organizer: 第36回長崎感染症研究会
  • Place of Presentation: 長崎大学(良順会館)
  • Year and Date: 2012-03-24
• [Presentation] プリオン感染におけるInterferon regulatory factor-3(IRF-3)遺伝子の発現抑制機序の解明 (2011)
  • Author(s): 本間拓二郎、石橋大輔、西田教行
  • Organizer: 第35回長崎感染症研究会
  • Place of Presentation: 長崎大学(良順会館)
  • Year and Date: 2011-03-26
• [Presentation] プリオン感染におけるInterferon regulatory factor-3 (IRFL3)遺伝子の発現抑制機序の解明 (2011)
  • Author(s): 本間拓二郎、石橋大輔、西田教行
  • Organizer: 第35回 長崎感染症研究会
  • Place of Presentation: 長崎大学(良順会館)
  • Year and Date: 2011-03-26
• [Presentation] 細菌由来の蛋白succinylarginine dihydrolaseの免疫によるプリオン病の予防効果. Immunization with bacterial enzyme succinylarginine dihydrolase induces antigenic mimicry-mediated anti-prion effects in mice (2011)
  • Author(s): Daisuke Ishibashi, Hitoki Yamanaka, Naohiro Yamaguchi, Yoshitaka Yamaguchi, Noriyuki Nishida. Suehiro Sakaguchi
  • Organizer: 第40回日本免疫学会総会
  • Place of Presentation: 幕張メッセ
• [Presentation] Analysis of murine interferon regulatory factor-3(IRF-3) promoter. IUMS 2011 (2011)
  • Author(s): Takujiro Homma, Daisuke Ishibashi, Noriyuki Nishida
  • Organizer: Meetings of the Three Divisions of the International Union of Microbiological Societies 2011
  • Place of Presentation: Sapporo
• [Presentation] Protective Role of Interferon Regulatory Factor 3-Dependent Signaling Against Prion Infection (2011)
  • Author(s): Daisuke Ishibashi, Noriyuki Nishida
  • Organizer: Prion 2011
  • Place of Presentation: Montreal, Canada
• [Presentation] Bio.072 : Protective role of interferon regulatory factor 3-dependent signaling against priori infection (2011)
  • Author(s): Daisuke Ishibashi, Noriyuki Nishida
  • Organizer: Prion 2011
  • Place of Presentation: Montreal (Canada)
• [Presentation] Analysis of murine interferon regulatory factor-3 (IRF-3) promoter (2011)
  • Author(s): Takujiro Homma, Daisuke Ishibashi, Noriyuki Nishida
  • Organizer: IUMS 2011 (Meetings of the Three Divisions of the International Union of Microbiological Societies 2011)
  • Place of Presentation: Sapporo (Japan)
• [Presentation] 細菌由来の蛋白succinylarginine dihydrolaseの免疫によるプリオン病の予防効果/Immunization with bacterial enzyme succinylarginine dihydrolase induces antigenic mimicrymediated anti-prion effects in mice (2011)
  • Author(s): 石橋大輔, 山中仁木, 山口尚宏, 山口仁孝, 西田教行, 坂口末廣
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉)
• [Presentation] Reconstruction of infectious prion protein in vitro (2010)
  • Author(s): Kazunori Sano, Ryuichiro Atarashi, Daisuke Ishibashi, Naohiro Yamaguchi, Takayuki Fuse, Noriyuki Nishida
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] FK506はプリオン感染マウスの生存期間を延長させる (2010)
  • Author(s): 中垣岳大, 佐藤克也, 鎌足雄司, 新竜一郎, 石橋大輔, 山口尚宏, 西田教行
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] Reconstruction of infectious prion protein in vitro (2010)
  • Author(s): Kazunori Sano, ryuichiro atarashi, daisuke ishibashi, naohiro yamaguchi, takayuki fuse, noriyuki nishida
  • Organizer: BMB2010 第33回日本分子生物学会年会 第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] FK506はプリオン感染マウスの生存期間を延長させる (2010)
  • Author(s): 中垣岳大, 佐藤克也, 鎌足雄司, 新竜一郎, 石橋大輔, 山口尚宏, 西田教行
  • Organizer: BMB2010 第33回日本分子生物学会年会 第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
• [Remarks]
  • URL: http://www.med.nagasaki-u.ac.jp/mmi/cmb/index.html
• [Remarks]
  • URL: http://www.med.nagasaki-u.acjp/mmi/cmb/index.html
• [Remarks]
  • URL: http://www.med.nagasaki-u.ac.jp/mmi/cmb/index.html