A role of nuclear import activity on the proliferation of cutaneous diseases

• Project/Area Number: 22791070
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Osaka University
• Principal Investigator: UMEGAKI Noriko 大阪大学, 医学系・研究科, 助教 (80397629)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 核移行シグナル / karyopherinα2 / TAP methods / mass spectrometry / MTSアッセイ / karyopherin α / 増殖 / LC/MS/MS

Research Abstract

Detection and analysis of target proteins imported by karyopherin alpha 2(KPNA2) in HaCaT cells using tandem affinity purification method and mass spectrometry to investigate the roles of KPNA2 in the cell growth. Immunohistochemical staining using anti-KPNA2 antibody was performed in the skin diseases including psoriasis and squamous cell carcinomas to demonstrate the relation ship between KPNA2 and keratinocyte proliferation.

Research Products

• [Journal Article] Pregnancy and childbirth in three mothers with recessive dystrophic epidermolysis bullosa (2012)
  • Author(s): Hanafusa T, Tamai K, Umegaki N, Yamaguchi Y, Fukuda S, Nishikawa Y, Yaegashi N, Okuyama R, McGrath JA, Katayama I
  • Journal Title: Clinical and experimental dermatology Volume: 37(1) Pages: 10-4
  • Peer Reviewed
• [Journal Article] Hereditary angioedema in Japan : Genetic analysis of 13 unrelated cases (2012)
  • Author(s): Yamamoto T, Horiuchi T, Miyahara H, Yoshizawa S, Maehara J, Shono E, Takamura K, Machida H, Tsujioka K, Kaneko T, Uemura N, Suzawa K, , Inagaki N, Umegaki N, Kasamatsu Y, Hara A, Arinobu Y, Inoue Y, Niiro H, Kashiwagai Y, Harashima S, Tahira T, Tsukamoto
  • Journal Title: The American Journal of the Medical Sciences Volume: 343(3) Pages: 210-4
  • Peer Reviewed
• [Journal Article] The course of Pregnancy and childbirth in three mothers with recessive dystrophic epidermolysis bullosa (2012)
  • Author(s): Hanafusa T, Tamai K, Umegaki N, Yamaguchi Y, Fukuda S, Nishikawa Y, Yaegashi N, Okuyama R, McGrath JA, Katayama I
  • Journal Title: Clin Exp Dermatol Volume: 37(1) Pages: 10-4
  • Peer Reviewed
• [Journal Article] Kitaba S, Matsui S, Iimuro E, Nishioka M, Kijima A, Umegaki N, Murota H, Katayama I (2011)
  • Author(s): Kitaba S, Matsui S, Iimuro E, Nishioka M, Kijima A, Umegaki N, Murota H, Katayama I
  • Journal Title: Link between Dry Skin and Autoimmune Anhidrosis Volume: 60(3) Pages: 387-91
  • Peer Reviewed
• [Journal Article] Vorner type palmoplantar keratoderma : novel KRT9 mutation associated with knuckle pad-like lesion and recurrent mutation causing digital mutilation (2011)
  • Author(s): Umegaki N, Nakano H, Tamai K, Mitsuhashi Y, Akasaka E, Sawamura D, Katayama I
  • Journal Title: Br J Dermatol Volume: 165(1) Pages: 199-201
  • Peer Reviewed
• [Journal Article] PDGFR alpha-positive cells in bone marrow are mobilized by high mobility group box 1(HMGB1) to regenerate injured epithelia (2011)
  • Author(s): Tamai K, Yamazaki T, Chino T, Ishii M, Otsuru S, Kikuchi Y, Iinuma S, Saga K, Nimura K, Shimbo T, Umegaki N, Katayama I, Miyazaki J, Takeda J, McGrath JA, Uitto J, Kaneda Y
  • Journal Title: Proc Natl Acad Sci Volume: 108(16) Pages: 6609-14
  • Peer Reviewed
• [Journal Article] A case of bullous pemphigoid exacerbated by irradiation after breast conservative radiotherapy (2011)
  • Author(s): Isohashi F, Konishi K, Umegaki N, Tanei T, Koizumi M, Yoshioka Y
  • Journal Title: Jpn J Clin Oncol Volume: 41(6) Pages: 811-3
  • Peer Reviewed
• [Journal Article] A case of giant squamous cell carcinoma of the buttock possibly arose from syringocystadenoma and invaded to the rectum (2011)
  • Author(s): Nishioka M, Tanemura A, Yamanaka T, Umegaki N, Tani M, Katayama I, Takemasa I, Sekimoto M, Tomita K, Tamai N
  • Journal Title: J Skin Cancer Volume: online journal Pages: 21340-21340
  • Peer Reviewed
• [Journal Article] Four Cases of Atopic Dermatitis Complicated by Sjogren's Syndrome : Link between Dry Skin and Autoimmune Anhidrosis (2011)
  • Author(s): Kitaba S, Matsui S, Iimuro E, Nishioka M, Kijima A, Umegaki N, Murota H, Katayama I
  • Journal Title: Allergol Int Volume: 60(3) Pages: 387-91
  • Peer Reviewed
• [Journal Article] PDGFRalpha-positive cells in bone marrow are mobilized by high mobility group box 1 (HMGB1)to regenerate injured epithelia (2011)
  • Author(s): Tamai K, Yamazaki T, Chino T, Ishii M, Otsuru S, Kikuchi Y, Iinuma S, Saga K, Nimura K, Shimbo T, Umegaki N, Katayama I, Miyazaki J, Takeda J, McGrath JA, Uitto J, Kaneda Y
  • Journal Title: Proc Natl Acad Sci Volume: 108(16) Pages: 6609-14
  • Peer Reviewed
• [Journal Article] Clinical implications of leukocytapheresis using a centrifugal cell separator for steroid-resistant pyoderma gangrenosum associated with inf lammatory bowel disease (2011)
  • Author(s): Hanafusa T, Azukizawa H, Umegaki N, Tani M, Yamaguchi Y, Katayama I
  • Journal Title: J Dermatol Volume: 38(5) Pages: 507-510
  • Peer Reviewed
• [Journal Article] PDGFRalpha-positive cells in bone marrow are mobilized by high mobility group box 1 (HMGB1) to regenerate injured epithelia (2011)
  • Author(s): Tamai K, Yamazaki T, Chino T, Ishii M, Otsuru S, Kikuchi Y, Iinuma S, Saga K, Nimura K, Shimbo T, Umegaki N, Katayama I, Miyazaki J, Takeda J, McGrath JA, Uitto J, Kaneda Y
  • Journal Title: Proc Natl Acad Sci Volume: 108(16) Pages: 6609-14
  • Peer Reviewed
• [Journal Article] Hereditary angioedema in Japan : Genetic analysis of 13 unrelated cases (2011)
  • Author(s): Yamamoto T, Horiuchi T, Miyahara H, Yoshizawa S, Maehara J, Shono E, Takamura K, Machida H, Tsujioka K, Kaneko T, Uemura N, Suzawa K,, Inagaki N, Umegaki N, Kasamatsu Y, Hara A, Arinobu Y, Inoue Y, Niiro H, Kashiwagai Y, Harashima S, Tahira T, Tsukamoto H, Akashi K
  • Journal Title: The American Journal of the Medical Sciences Volume: (In press)
  • Peer Reviewed
• [Journal Article] Clinical implications of leukocytapheresis using a centrifugal cell separator for steroid-resistant pyoderma gangrenosum associated with inflammatory bowel disease (2011)
  • Author(s): Hanafusa T, Azukizawa H, Umegaki N, Tani M, Yamaguchi Y, Katayama I
  • Journal Title: J Dermatol Volume: 2010.01009
  • Peer Reviewed
• [Journal Article] Pregnancy and childbirth in three mothers with recessive dystrophic epidermolysis bullosa (2011)
  • Author(s): Hanafusa T, Tamai K, Umegaki N, Yamaguchi Y, Fukuda S, Nishikawa Y, Yaegashi N, Okuyama R, McGrath JA, Katayama I
  • Journal Title: Clinical and experimental dermatology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Clinical implications of leukocytapheresis using a centrifugal cell separator for steroid-resistant pyoderma gangrenosum associated with inflammatory bowel disease (2010)
  • Author(s): Hanafusa T, Azukizawa H, Umegaki N, TaniM, Yamaguchi Y, Katayama I
  • Journal Title: J Dermatol Volume: 38(5) Pages: 507-10
  • Peer Reviewed
• [Journal Article] Relation between the expression levels of thePOU transcription factors Skn-1a and Skn-1n and keratinocyte differentiation (2010)
  • Author(s): Takemoto H, Tamai K, Akasaka E, Rokunohe D, Takiyoshi N, Umegaki N, Nakajima K, Aizu T, Kaneko T, Nakano H, Sawamura D
  • Journal Title: J Dermatol Sci Volume: 60(3) Pages: 203-5
  • Peer Reviewed
• [Journal Article] Impact of sedative and non-sedative antihistamineson the impaired productivity and quality of life in patients with pruritic skin diseases (2010)
  • Author(s): Murota H, Kitaba S, Tani M, Wataya-Kaneda M, Azukizawa H, Tanemura A, Umegaki N, Terao M, Kotobuki Y, Katayama I
  • Journal Title: Allergol Int Volume: 59(4) Pages: 345-54
  • Peer Reviewed
• [Journal Article] Good' s syndrome(hypogammaglobulinemia with thymoma) presenting intractable opportunistic infections and hyperkeratotic lichen planus (2010)
  • Author(s): Hanafusa T, Umegaki N, Yamaguchi Y, Katayama I
  • Journal Title: J Dermatol Volume: 37(2) Pages: 171-4
  • Peer Reviewed
• [Journal Article] 肛門病変を契機に診断した直腸肛門部悪性黒色腫の1例 (2010)
  • Author(s): 中森利枝、西村由佳理、梅垣知子、谷守、種村篤、片山一朗、濱野梨絵、竹政伊知郎、関本貢嗣
  • Journal Title: 皮膚の科学 Volume: 9(3) Pages: 244-249
  • NAID: 130004547419
  • Peer Reviewed
• [Presentation] Investigation of roles and mechamisims of karyopherin alpha2 in keratinocyte proliferative disorders (2010)
  • Author(s): Noriko Umegaki, Katsuto Tamai, Keisuke Nimura, Satoshi Serada, Tetsuji Naka, Takehiko Yamazaki, Hajime Nakano, Yasufumi Kaneda and Ichiro Katayama
  • Organizer: 40^<th> ESDR
  • Place of Presentation: ヘルシンキ,フィンランド
  • Year and Date: 2010-09-08
• [Presentation] Voerner型掌蹠角化症の遺伝子変異解析と病因論的意義 (2010)
  • Author(s): 梅垣知子、中野創、吉良正浩、澤村大輔、片山一朗
  • Organizer: 第109回日本皮膚科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-04-16