Analysis and regulation of proliferative and migratory capacities of epidermal keratinocytes

• Project/Area Number: 22791080
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Ehime University
• Principal Investigator: NANBA Daisuke 愛媛大学, 上級研究員センター, 講師 (10380255)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 皮膚炎症・再生学 / 表皮角化幹細胞 / 表皮角化細胞 / 幹細胞 / コロニー / 創傷治癒

Research Abstract

We examined the association of proliferative and migratory capacities of human cultured epidermal keratinocytes and found that proliferative capacity was positively correlated with migratory capacity in keratinocytes. We also found that a kind of integrin was involved in migratory capacity of proliferative keratinocytes and that a cell behavior generated collective dynamics in the keratinocyte colony. This result leads to understanding the collective behaviors of cells with higher proliferative capacity including stem cells and tumor cells.

Research Products

• [Journal Article] Ectodomain shedding and remnant peptide signaling of EGFRs and their ligands (2011)
  • Author(s): Higashiyama S, Nanba D, Nakayama H, Inoue H, Fukuda S
  • Journal Title: Journal of Biochemistry Volume: Vol.150 Pages: 15-22
  • NAID: 10030586134
  • Peer Reviewed
• [Presentation] Switching modes of keratinocyte response to EGF through clonal conversion by actin filament remodeling (2011)
  • Author(s): 難波大輔
  • Organizer: 日本研究皮膚科学会
  • Place of Presentation: 宜野湾市
  • Year and Date: 2011-12-10
• [Presentation] #1.Kinetic biology on stem cell colonies and their geometrical shapes : why stem cells make round colonies? #2.Switching modes of keratinocyte response to EGF through clonal conversion by actin filament remodeling (2011)
  • Author(s): 難波大輔
  • Organizer: 日本研究皮膚科学会
  • Place of Presentation: 宜野湾市
  • Year and Date: 2011-12-10
• [Presentation] Kinetic biology on stem cell colonies and their geometrical shapes : why stem cells make round colonies? (2011)
  • Author(s): 難波大輔
  • Organizer: 日本発生生物学会
  • Place of Presentation: 京都市
  • Year and Date: 2011-05-20
• [Presentation] #1.Kinetic biology on stem cell colonies and their geometrical shapes : why stem cells make round colonies? #2.Switching modes of keratinocyte response to EGF through clonal conversion by actin filament remodeling (2011)
  • Author(s): 難波大輔
  • Organizer: 日本発生生物学会
  • Place of Presentation: 京都市
  • Year and Date: 2011-05-20
• [Presentation] Collective migration of keratinocytes induced by EGF requires their proliferative capacity (2010)
  • Author(s): 難波大輔
  • Organizer: 日本研究皮膚科学会
  • Place of Presentation: 和歌山県民文化会館
  • Year and Date: 2010-12-03
• [Presentation] Collective migration of keratinocytes induced by EGF requires their proliferative capacity (2010)
  • Author(s): 難波大輔
  • Organizer: 国際分化学会
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2010-11-16
• [Presentation] Collective migration of keratinocytes induced by EGF requires their proliferative capacity (2010)
  • Author(s): 難波大輔
  • Organizer: 日本発生生物学会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-06-20
• [Remarks]
  • URL: http://www.ccr.ehime-u.ac.jp/srf/
• [Remarks]
  • URL: http://www.ccr.ehime-u.ac.jp/srf/
• [Remarks]
  • URL: http://www.ccr.ehime-u.ac.jp/srf/