Mechanisms underlying effects of BDNF and antidepressants on microglia ; Possible role of TRPC channels(MIZOGUCHI YOSHITO)

• Project/Area Number: 22791128
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Psychiatric science
• Research Institution: Saga University
• Principal Investigator: MIZOGUCHI Yoshito 佐賀大学, 医学部, 講師 (60467892)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: うつ病 / BDNF / ミクログリア / 神経炎症 / 薬理学

Research Abstract

In rodent microglial cells, sustained activation of SOCE, possibly mediated by TRPC3 channels, occurred after brief BDNF application and contributed to the maintenance of sustained[ Ca^<2+>] i elevation. BDNF acutely upregulates the surface expression of TRPC3 channels in rodent microglia. In addition, RT-PCR and immunocytochemical techniques revealed that TRPC3 channels are highly expressed in rodent microglial cells.

Research Products

• [Journal Article] The effect of oral presentation on salivary 3-methoxy-4-hydroxy-phenylglycol(MHPG) and cortisol concentrations in training doctors : a preliminary study. (2012)
  • Author(s): Mizoguchi Y, Sakami A, Imamura Y, Tsuruta T, Egami M, Yamada S
  • Journal Title: Endocrine Volume: (in press)
• [Journal Article] Neuroinflammation in schizophrenia especially focused on the role of microglia (2012)
  • Author(s): Monji A, Kato TA, Mizoguchi Y, Horikawa H, Seki Y, Kasai M, Yamauchi Y, Yamada S, Kanba S
  • Journal Title: Prog Neuropsychopharmacol Biol Psychiatry Volume: (in press)
• [Journal Article] 神経炎症の観点よりみたアルツハイマー病の病態生理とその治療 (2012)
  • Author(s): 門司晃、溝口義人、山田茂人
  • Journal Title: 分子精神医学 Volume: 12(1) Pages: 9-13
• [Journal Article] Baseline saliva level of 3-methoxy-4-hydroxyphenylglycole(MHPG) associates with a consequent cognitive decline in non-demented elderly subjects : three-years follow-up study (2012)
  • Author(s): Watanabe I, Li GY, Imamura Y, Nabeta H, Kunitake Y, Ishii H, Haraguchi M, Furukawa Y, Tateishi H, Kojima N, Mizoguchi Y, Yamada S
  • Journal Title: Psychiatry Res Volume: 195(3) Pages: 125-8
• [Journal Article] Possible role of BDNF-induced microglial intracellular Ca(2+) elevation in the pathophysiology of neuropsychiatric disorders. (2011)
  • Author(s): Mizoguchi Y, Monji A, Kato TA, Horikawa H, Seki Y, Kasai M, Kanba S, Yamada S
  • Journal Title: Mini Rev Med Chem Volume: 11 Pages: 575-81
• [Journal Article] Anti-Inflammatory properties of antipsychotics via microglia modulations : are antipsychotics a' fire extinguisher' in the brain of schizophrenia? (2011)
  • Author(s): Kato TA, Monji A, Mizoguchi Y, Hashioka S, Horikawa H, Seki Y, Kasai M, Utsumi H, Kanba S
  • Journal Title: Mini Rev Med Chem Volume: 11 Pages: 565-74
• [Journal Article] Aripiprazole inhibits superoxide generation from phorbol-myristate-acetate(PMA)-stimulated microglia in vitro : implication for antioxidative psychotropic actions via microglia (2011)
  • Author(s): Kato TA, Monji A, Yasukawa K, Mizoguchi Y, Horikawa H, Seki Y, Hashioka S, Han YH, Kasai M, Sonoda N, Hirata E, Maeda Y, Inoguchi T, Utsumi H, Kanba S
  • Journal Title: Schizophr Res Volume: 129(2-3) Pages: 172-82
• [Journal Article] Inhibitory effects of SSRIs on IFN-γinduced microglial activation through the regulation of intracellular calcium (2011)
  • Author(s): Horikawa H, Kato TA, Mizoguchi Y, Monji A, Seki Y, Ohkuri T, Gotoh L, Yonaha M, Ueda T, Hashioka S, Kanba S
  • Journal Title: Prog Neuropsychopharmacol Biol Psychiatry Volume: 34(7) Pages: 1306-16
• [Journal Article] Lewis DA(2010) Development of cannabinoid 1 receptor protein and messenger RNA in monkey dorsolateral prefrontal cortex (2011)
  • Author(s): Eggan SM*, Mizoguchi Y*, Stoyak SR
  • Journal Title: Cereb Cortex Volume: 20(5) Pages: 1164-74
• [Journal Article] Aripiprazole inhibits superoxide generation from phorbol-myristate-acetate (PMA)-stimulated microglia in vitro : implication for antioxidative psychotropic actions via microglia (2011)
  • Author(s): Kato TA, et al
  • Journal Title: Schizophr Res Volume: 129 Pages: 172-182
  • Peer Reviewed
• [Journal Article] Anti-Inflammatory properties of antipsychotics via microglia modulations : are antipsychotics a 'fire extinguisher' in the brain of schizophrenia? (2011)
  • Author(s): Kato TA, et al
  • Journal Title: Mini Rev Med Chem Volume: 11 Pages: 565-574
  • Peer Reviewed
• [Journal Article] Possible role of BDNF-induced microglial intracellular Ca(2+) elevation in the pathophysiology of neuropsychiatric disorders (2011)
  • Author(s): Mizoguchi Y, et al
  • Journal Title: Mini Rev Med Chem Volume: 11 Pages: 575-585
  • Peer Reviewed
• [Journal Article] Inhibitory effects of SSRIs on interferon-γ-induced microglial activation through the intracellular calcium (2010)
  • Author(s): Horikawa H, et al
  • Journal Title: Progress in Neuropsychopharamacology, Biological Psychiatry Volume: 34 Pages: 1306-1316
  • Peer Reviewed
• [Journal Article] Development of cannabinoid 1 receptor protein and messenger RNA in monkey dorsolateral prefrontal cortex (2010)
  • Author(s): Eggan SM, Mizoguchi Y, et al (Equal author contribution)
  • Journal Title: Cerebral Cortex Volume: 20 Pages: 1164-1174
  • Peer Reviewed
• [Presentation] SSRI potentiates BDNF-induced microglial intracellular Ca^<2+> elevation Society for Neuroscience (2011)
  • Author(s): Mizoguchi Y et al
  • Organizer: 41st Annual Meeting
  • Place of Presentation: Washington Convention Center
  • Year and Date: 2011-11-15
• [Presentation] SSRI potentiates BDNF-induced microglial intracellular Ca2+ elevation (2011)
  • Author(s): Mizoguchi Y, et al
  • Organizer: Society for Neuroscience, 41st Annual Meeting
  • Place of Presentation: Washington Convention Center
  • Year and Date: 2011-11-15
• [Presentation] 精神疾患におけるミクログリアの役割 (2011)
  • Author(s): 神庭重信ら
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-15
• [Presentation] Possible role of BDNF-induced microglial intracellular Ca^<2+> elevation in the pathophysiology of neuropsychiatric disorders (2010)
  • Author(s): Mizoguchi Y et al
  • Organizer: 40th annual meeting of the Society for Neuroscience 2010 in San Diego
  • Place of Presentation: San Diego Convention Center
  • Year and Date: 2010-11-17
• [Presentation] Possible role of BDNF-induced microglial intracellular Ca2+ elevation in the pathophysiology of neuropsychiatric disorders (2010)
  • Author(s): Mizoguchi Y, et al
  • Organizer: 40th annual meeting of the Society for Neuroscience 2010 in San Diego
  • Place of Presentation: San Diego Convention Center
  • Year and Date: 2010-11-17