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Clock genes analysis in human breast cancer

Research Project

Project/Area Number 22791243
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionHirosaki University

Principal Investigator

MOROHASHI Satoko  弘前大学, 医学(系)研究科(研究院), 助教 (90569592)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywords時計遺伝子 / 乳癌 / 治療 / アポトーシス / DEC / 転写因子
Research Abstract

Expression of clock genes, DEC1 and DEC2 were up-regulated in the MCF-7 human breast cancer cells by TNF-α in a concentration-dependent manner. TNF-α induced the apoptosis of MCF-7 cells. We also demonstrated that siRNA-mediated DEC2 knockdown, but not DEC1, increased the Bax expression, cleavage PARP and cleavage caspase-7 in MCF-7 cells. In conclusion, we speculated that DEC2 plays an important role in the regulation of both apoptosis and proliferation of the MCF-7 cells.
In human breast cancer MCF-7 cells, paclitaxel increased the expression of DEC1 and DEC2. However, DEC1 and DEC2 exhibited opposite effects in paclitaxel inducing apoptosis, That is DEC1 has pro-apoptotic, while DEC2 has anti-apoptotic function. Both the two transcription factors play important roles in regulating paclitaxel induced apoptosispathway.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (36 results)

All 2012 2011 2010

All Journal Article (20 results) (of which Peer Reviewed: 20 results) Presentation (12 results) Book (4 results)

  • [Journal Article] The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer2012

    • Author(s)
      Wu, Y., Kawamoto, T., Noshiro, M., et al.(他10名)
    • Journal Title

      Int J Oncol

      Volume: 41 Issue: 4 Pages: 1337-1346

    • DOI

      10.3892/ijo.2012.1559

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The basic helix-loop-helix transcription factor DEC2 inhibits TGF-beta-induced tumor progression in human pancreatic cancer BxPC-3 cells2012

    • Author(s)
      Sato, F., Kawamura, H., Kawamoto, T., Noshiro, M.,(他10名)
    • Journal Title

      Int J Mol Med

      Volume: 30 Issue: 3 Pages: 495-501

    • DOI

      10.3892/ijmm.2012.1037

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] BHLH transcription factor DEC2 regulates pro-apoptotic factor Bim in human oral cancer HSC-3 cells.2012

    • Author(s)
      Wu Y, Sato F, Bhawal UK, Kawamoto T, Fujimoto K, Noshiro M, Seino H, Morohashi S, Kato Y, Kijima H
    • Journal Title

      Biomed Res

      Volume: 33(2) Pages: 75-82

    • NAID

      130004470811

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Smad3 and Snail show circadian expression in human gingival fibroblasts, human mesenchymal stem cell, and in mouse liver2012

    • Author(s)
      Sato, F., Wu, Y., Noshiro, M.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 419 Issue: 2 Pages: 441-446

    • DOI

      10.1016/j.bbrc.2012.02.076

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epithelial cell adhesion molecule (EpCAM) overexpression is correlated with malignant potentials of intraductal papillary mucinous neoplasms (IPMNs) of the pancreas.2012

    • Author(s)
      Yonaiyama S
    • Journal Title

      Biomed Res

      Volume: 34 Pages: 87-95

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] BHLH transcription factor DEC2 regulates pro-apoptotic factor Bim in human oral cancer HSC-3 cells.2012

    • Author(s)
      Wu Y
    • Journal Title

      Biomed Res.

      Volume: 33 Pages: 75-82

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intimal sarcoma of the pulmonary artery--diagnostic challenge.2011

    • Author(s)
      Fukuda W, Morohashi S, Fukuda I
    • Journal Title

      Acta Cardiol

      Volume: 66(4) Pages: 539-41

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Histopathological phenotypes of early gastric cancer and its background mucosa.2011

    • Author(s)
      Kudo Y, Morohashi S, Takasugi K, Tsutsumi S, Ogasawara H, Hanabata N, Yoshimura T, Sato F, Fukuda S, Kijima H
    • Journal Title

      Biomed Res

      Volume: 32(2) Pages: 127-34

    • NAID

      130004470772

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] PERIOD1 (PER1) has anti-apoptotic effects, and PER3 has pro-apoptotic effects during cisplatin (CDDP) treatment in human gingival cancer CA9-22 cells.2011

    • Author(s)
      Sato F, Wu Y, Bhawal UK, Liu Y, Imaizumi T, Morohashi S, Kato Y, Kijima H
    • Journal Title

      Eur J Cancer

      Volume: 47(11) Issue: 11 Pages: 1747-58

    • DOI

      10.1016/j.ejca.2011.02.025

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Basic helix-loop-helix transcription factors DEC1 and DEC2 regulate the paclitaxel-induced apoptotic pathway of MCF-7 human breast cancer cells.2011

    • Author(s)
      Wu Y, Sato F, Bhawal UK, Kawamoto T, Fujimoto K, Noshiro M, Morohashi S,Kato Y, Kijima H.
    • Journal Title

      Int J MolMed

      Volume: 27(4) Pages: 491-495

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] PERIOD1 (PER1) has anti-apoptotic effects, and PER3 has pro-apoptotic effects during cisplatin (CDDP) treatment in human gingival cancer CA9-22 cells2011

    • Author(s)
      Sato F, Wu Y, Bhawal UK, Liu Y, Imaizumi T, Morohashi S, Kato Y, Kijima H
    • Journal Title

      Eur J Cancer

      Volume: 47 Pages: 1747-1758

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Basic helix-loop-helix transcription factors DEC1 and DEC2 regulate the paclitaxel-induced apoptotic pathway of MCF-7 human breast cancer cells2011

    • Author(s)
      Wu Y, Sato F, Bhawal UK, Kawamoto T, Fujimoto K, Noshiro M, Morohashi S, Kato Y, Kijima H
    • Journal Title

      Int J Mol Med.

      Volume: 27 Pages: 491-495

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anti-apoptotic effect of claudin-1 in tamoxifen-treated human breast cancer MCF-7 cells.2010

    • Author(s)
      Akasaka H, Sato F, Morohashi S, Wu Y, Liu Y, Kondo J, Odagiri H, Hakamada K, Kijima H
    • Journal Title

      BMC Cancer

      Volume: 10 Issue: 1 Pages: 548-548

    • DOI

      10.1186/1471-2407-10-548

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vimentin expression of esophageal squamous cell carcinoma and its aggressive potential for lymph node metastasis.2010

    • Author(s)
      Jin H, Morohashi S, Sato F, Kudo Y, Akasaka H, Tsutsumi S, Ogasawara H, Miyamoto K, Wajima N, Kawasaki H, Hakamada K, Kijima H
    • Journal Title

      Biomed Res

      Volume: 31(2) Pages: 105-112

    • NAID

      130004903787

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells.2010

    • Author(s)
      Liu Y, Sato F, Kawamoto T, Fujimoto K, Morohashi S, Akasaka H, Kondo J, Wu Y, Noshiro M, Kato Y, Kijima H
    • Journal Title

      Genes Cells

      Volume: 15(4) Pages: 315-25

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells.2010

    • Author(s)
      Liu Y, Sato F, Morohashi S, et al.
    • Journal Title

      Genes to Cells

      Volume: Vol.15 Pages: 315-325

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vimentin expression of esophageal squamous cell carcinoma and its aggressive potential for lymph node metastasis.2010

    • Author(s)
      Jin H, Morohashi S, Sato F, et al.
    • Journal Title

      Biomedical Research

      Volume: Vol.31 Pages: 105-112

    • NAID

      130004903787

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anti-apoptotic effect of claudin-1 in tamoxifen-treated human breast cancer MCF-7 cells.2010

    • Author(s)
      Akasaka H, Sato F, Morohashi S, et al.
    • Journal Title

      BMC Cancer

      Volume: Vol.10 Pages: 548-548

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 日本病理学会2010

    • Author(s)
      羽賀敏博、諸橋聡子、佐藤冬樹, など
    • Journal Title

      生体肝移植術が施行された成人発症II型シトルリン血症の1例

      Volume: 27 Pages: 100-104

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 日本病理学会2010

    • Author(s)
      赤坂治枝、諸橋聡子、神寛之、堤伸二、大橋大成、工藤康之、原精一、佐藤冬樹, など
    • Journal Title

      甲状腺混合性髄様・濾胞細胞癌の1例

      Volume: 27 Pages: 115-118

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] PERIOD is involved in the proliferation of human pancreatic MIA-PaCa2 cancer cells.2012

    • Author(s)
      Takahiro Suzuki
    • Organizer
      第9回日本病理学会カンファレンス 2012山口
    • Place of Presentation
      山口
    • Related Report
      2012 Annual Research Report
  • [Presentation] DEC expression and cell proliferation of human breast MCF-7 cancer cells on typeI collagen-coated dishes.2012

    • Author(s)
      Yuta Tsugeno
    • Organizer
      第9回日本病理学会カンファレンス 2012山口
    • Place of Presentation
      山口
    • Related Report
      2012 Annual Research Report
  • [Presentation] 異型濾胞上皮の結節性増生を示したGraves病甲状腺の一例2011

    • Author(s)
      諸橋聡子
    • Place of Presentation
      東京
    • Year and Date
      2011-11-18
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト乳癌細胞株MCF-7におけるbHLH型転写因子DEC2のアポトーシス制御機構の解析2011

    • Author(s)
      諸橋聡子
    • Organizer
      日本乳癌学会
    • Place of Presentation
      仙台市
    • Year and Date
      2011-09-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 肺動脈内膜肉腫の1剖検例2011

    • Author(s)
      諸橋聡子
    • Organizer
      日本病理学会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-04-28
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト乳癌細胞株MCF-7におけるbHLH型転写因子DEC2のアポトーシス制御機構の解析2011

    • Author(s)
      諸橋聡子
    • Organizer
      第19回日本乳癌学会
    • Place of Presentation
      仙台市
    • Related Report
      2011 Annual Research Report
  • [Presentation] Basic helix-loop-helix (bHLH) transcription factpr DEC1 regulates EMT progression in human pancreatic cancer2011

    • Author(s)
      Wu Y, Sato F, Morohashi S, Kato Y, Kjima H
    • Organizer
      第70回日本癌学会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Annual Research Report
  • [Presentation] DEC1 has pro-apoptotic, and DEC2 has anti-apoptotic effects in paclitaxel-treated MCF-7 cells2011

    • Author(s)
      Wu Y, Sato F, Morohashi S, Kato Y, Kjima H
    • Organizer
      第8回日本病理学会カンファレンス
    • Place of Presentation
      松本
    • Related Report
      2011 Annual Research Report
  • [Presentation] 診断が困難であった乳腺症型繊維腺腫の1例の病理学的検討2010

    • Author(s)
      諸橋聡子
    • Organizer
      臨床外科学会
    • Place of Presentation
      横浜市
    • Year and Date
      2010-11-21
    • Related Report
      2012 Final Research Report
  • [Presentation] 診断が困難であった乳腺症型線維腺腫の1例の病理学的検討2010

    • Author(s)
      諸橋聡子
    • Organizer
      日本臨床外科学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2010-11-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 乳腺に発生した腺脂肪腫の1例2010

    • Author(s)
      諸橋聡子
    • Organizer
      日本乳癌学会
    • Place of Presentation
      札幌市
    • Year and Date
      2010-06-25
    • Related Report
      2012 Final Research Report
  • [Presentation] 乳腺に発生した腺脂肪腫の1例2010

    • Author(s)
      諸橋聡子
    • Organizer
      第18回日本乳癌学会学術総会
    • Place of Presentation
      ロイトン札幌(札幌市)
    • Year and Date
      2010-06-25
    • Related Report
      2010 Annual Research Report
  • [Book] 腫瘍病理鑑別診断アトラス食道癌2012

    • Author(s)
      諸橋聡子
    • Publisher
      文光堂
    • Related Report
      2012 Final Research Report
  • [Book] 腫瘍病理鑑別診断アトラス大腸癌2011

    • Author(s)
      諸橋聡子
    • Publisher
      文光堂
    • Related Report
      2012 Final Research Report
  • [Book] 前癌病変の病理 胆道癌の前癌病変の病理2010

    • Author(s)
      堤伸二、小笠原絋志、原精一、赤坂治枝、神寛之、工藤康之、諸橋聡子、佐藤冬樹, など
    • Total Pages
      9
    • Publisher
      医学書院
    • Related Report
      2010 Annual Research Report
  • [Book] 虫垂の腫瘍2010

    • Author(s)
      諸橋聡子、佐藤冬樹、鬼島宏
    • Total Pages
      6
    • Publisher
      先端医学社
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2023-03-16  

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