Clock genes analysis in human breast cancer
| Project/Area Number |
22791243
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Hirosaki University |
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Principal Investigator |
MOROHASHI Satoko 弘前大学, 医学(系)研究科(研究院), 助教 (90569592)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 時計遺伝子 / 乳癌 / 治療 / アポトーシス / DEC / 転写因子 |
|---|
| Research Abstract |
Expression of clock genes, DEC1 and DEC2 were up-regulated in the MCF-7 human breast cancer cells by TNF-α in a concentration-dependent manner. TNF-α induced the apoptosis of MCF-7 cells. We also demonstrated that siRNA-mediated DEC2 knockdown, but not DEC1, increased the Bax expression, cleavage PARP and cleavage caspase-7 in MCF-7 cells. In conclusion, we speculated that DEC2 plays an important role in the regulation of both apoptosis and proliferation of the MCF-7 cells.
In human breast cancer MCF-7 cells, paclitaxel increased the expression of DEC1 and DEC2. However, DEC1 and DEC2 exhibited opposite effects in paclitaxel inducing apoptosis, That is DEC1 has pro-apoptotic, while DEC2 has anti-apoptotic function. Both the two transcription factors play important roles in regulating paclitaxel induced apoptosispathway.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Vimentin expression of esophageal squamous cell carcinoma and its aggressive potential for lymph node metastasis.2010
Author(s)
Jin H, Morohashi S, Sato F, Kudo Y, Akasaka H, Tsutsumi S, Ogasawara H, Miyamoto K, Wajima N, Kawasaki H, Hakamada K, Kijima H
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Journal Title
Biomed Res
Volume: 31(2)
Pages: 105-112
NAID
Related Report
Peer Reviewed
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[Journal Article] Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells.2010
Author(s)
Liu Y, Sato F, Kawamoto T, Fujimoto K, Morohashi S, Akasaka H, Kondo J, Wu Y, Noshiro M, Kato Y, Kijima H
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Journal Title
Genes Cells
Volume: 15(4)
Pages: 315-25
Related Report
Peer Reviewed
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[Journal Article] 日本病理学会2010
Author(s)
羽賀敏博、諸橋聡子、佐藤冬樹, など
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Journal Title
生体肝移植術が施行された成人発症II型シトルリン血症の1例
Volume: 27
Pages: 100-104
Related Report
Peer Reviewed
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[Book] 虫垂の腫瘍2010
Author(s)
諸橋聡子、佐藤冬樹、鬼島宏
Total Pages
6
Publisher
先端医学社
Related Report
