| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
When colon cancer cells HT29 were transplanted with intestinal embryonic fibroblasts(IEFs) from the Foxf2-/-and wild-type mice, the efficiency and tumor size were highest in cotranslantation with the wild-type IEFs, higher in that with the Foxf2-/-IEFs compared to that without fibroblasts. I isolated RNas from IEFs from the Foxf2-/-and wild-type mice and applied to DNA microarray analysis. I found 23 genes were significantly higher in the wild-type compared to the Foxf2-/-mice. I chose one gene named Wnt5a and demonstrated that the Wnt5a-expressing fibroblasts increased the efficiency and tumor size of HT29 cell transplantation.
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