| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
In GK7C-IR and GK22C-IR, the resistance to imatinib had been increasing from 2. 56 to 3. 73 fold comparing with the parental cells. Expression of ABC transporters : ABCC2 and ABCC5 has been shown in imatinib resistant cell lines and associated with its capacity to export a broad range of cytotoxic drugs. The status of phosphorylation of key cell signaling pathways(receptor tyrosine kinase : KIT, PDGFRa and its downstream-signaling AKT and ERK1/2 or non-receptor tyrosine kinase : SRC) was analyzed in established cell lines. As a result, the phosphorylation of SRC and ERK1/2 was increased compare to wild type cells. These results suggested that in imatinib resistant cell, the growth and survival has been to improve by high expression of ABC transporter and activation of Src and Erk1/2.
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