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Airway administration of small silencing RNA oligoneucleotides in mice : transient airspace enlargement induced by VEGF specific blockade in lung.

Research Project

Project/Area Number 22791320
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionKeio University

Principal Investigator

TAKAHASHI Yusuke  慶應義塾大学, 医学部, 助教 (00445214)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords遺伝子翻訳阻害 / 肺気腫
Research Abstract

We report a readily available model of lung targeted VEGF depletion by airway administration of VEGF small inhibitory RNA oligonucleotides(siRNAs). In the present study, airway administration of VEGF siRNAs mixed in surface active material induced transient air space enlargement in the mouse lung morphologically resembling the previously reported models of pulmonary emphysema. The present model may be useful in dissecting the involvement of VEGF in pulmonary emphysema. Additionally, we found that airway administration of DCI, a combination of dexamethasone, 3'-5'-cyclic adenosine monophosphate, and isobutylmethylxanthine, which has been reported to induce differentiation of type II alveolar cells, attenuated the air space enlargement in this particular model, at least in part through the recovery of lung VEGF expression. We believe that the results of this study have broad implications in the field of respiratory physiology.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (15 results)

All 2011 2010

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (10 results)

  • [Journal Article] Fibrous stroma is associated with poorer prognosis in lung squamous cell carcinoma patients2011

    • Author(s)
      Takahashi Y, Ishii G, et al
    • Journal Title

      J Thorac Oncol

      Volume: 6(9) Pages: 1460-1467

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Airway a dministration of dexamethason, 3'-5'-cyclic adenosine mono-phosphate, and isobutyl methylxanthine facilitates compensatory lung growth in adult mice2011

    • Author(s)
      Takahashi Y, Izumi Y, et al
    • Journal Title

      Am J Physiol Lung Ce ll Mol Physiol

      Volume: 300(3)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Airway ademonistration of dexamethason, 3'-5'-cyclic adenosine mono--phosphate, and isobutyl methylxanthine facilitates compensatory lung growth in adult mice.2011

    • Author(s)
      Takahashi Y, Izumi Y, et al.
    • Journal Title

      Am J Physiol Lung Cell Mol Physiol

      Volume: 300(3)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thyroid transcription factor-1 influences the early phase of compensatory lung growth in adult mice2010

    • Author(s)
      Takahashi Y, Izumi Y, et al
    • Journal Title

      Am J Cr it Care Med

      Volume: 181(2) Pages: 1397-1406

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Thyroid transcription factor-1 influences the early phase of compensatory lung growth in adult mice.2010

    • Author(s)
      Takahashi Y, Izumi Y, et al.
    • Journal Title

      Am J Crit Care Med

      Volume: 15;181(2) Pages: 1397-1406

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] マウス代償性肺成長におけるDCI投与による促進効果2011

    • Author(s)
      高橋祐介、泉陽太郎
    • Organizer
      第64回日本胸部外科学会定期学術集会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-10-11
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス代償性肺成長におけるDCI投与による促進効果2011

    • Author(s)
      高橋祐介、泉陽太郎, ら
    • Organizer
      第64回日本胸部外科学会定期学術集会
    • Place of Presentation
      名古屋国際会議場(愛知)
    • Year and Date
      2011-10-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] 扁平上皮癌における間質の形態学的特徴と悪性度の相関2011

    • Author(s)
      高橋祐介、石井源一郎
    • Organizer
      第28回日本呼吸器外科学会総会
    • Place of Presentation
      大分
    • Year and Date
      2011-05-13
    • Related Report
      2011 Final Research Report
  • [Presentation] 扁平上皮癌における間質の形態学的特徴と悪性度の相関2011

    • Author(s)
      高橋祐介、石井源一郎, ら
    • Organizer
      第28回日本呼吸器外科学会総会
    • Place of Presentation
      別府ビーコンプラザ(大分)
    • Year and Date
      2011-05-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] 成体マウス肺の再生過程におけるthyroid transcription factor2011

    • Author(s)
      高橋祐介、泉陽太郎
    • Organizer
      第100回日本病理学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2011-04-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 成体マウス肺の再生過程におけるthyroid transcription factor2011

    • Author(s)
      高橋祐介、泉陽太郎, ら
    • Organizer
      第100回日本病理学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Year and Date
      2011-04-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] マウス代償性肺再生における肺胞形成は正常肺発生に類似する2010

    • Author(s)
      高橋祐介、泉陽太郎
    • Organizer
      第63回日本胸部外科学会定期学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2010-10-25
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス代償性肺再生における肺胞形成は正常肺発生に類似する2010

    • Author(s)
      高橋祐介、泉陽太郎, ほか
    • Organizer
      日本胸部外科学会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2010-10-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] 難治性気胸に対するフェンタニル塩酸塩を用いた局所麻酔下手術の二例2010

    • Author(s)
      高橋祐介、泉陽太郎
    • Organizer
      第27回日本呼吸器外科学会総会
    • Place of Presentation
      仙台
    • Year and Date
      2010-05-13
    • Related Report
      2011 Final Research Report
  • [Presentation] 難治性気胸に対するフェンタニル塩酸塩を用いた局所麻酔下手術の二例2010

    • Author(s)
      高橋祐介、泉陽太郎, ほか
    • Organizer
      日本呼吸器外科学会
    • Place of Presentation
      仙台国際会議場
    • Year and Date
      2010-05-13
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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