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Vaccine therapy using EPC with gene modification

Research Project

Project/Area Number 22791332
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionUniversity of Tsukuba

Principal Investigator

ISHIKAWA Eiichi  筑波大学, 医学医療系, 講師 (30510169)

Research Collaborator 坂本 規彰  University of Bonn, Department of Neuropathology, 留学生
上前 洋二  筑波大学, 大学院人間総合科学研究科, 大学院・・生
松田 真秀  筑波大学, 附属病院, 病院講師
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords血管内皮前駆細胞 / 免疫学 / 脳神経疾患 / 悪性脳腫瘍 / 神経膠腫 / 脳神経外科 / 癌幹細胞 / ワクチン / 血管内皮細胞 / ワクチン療法 / 脳腫瘍
Research Abstract

Various types of endothelial cell-targeting therapies have been developed today. The present study investigated the effect of combined vaccine from glioma cells (GL261) and endothelial cells (F-2). Tumor growth was inhibited after preventive use of the combined vaccine, prepared from GL261 and F-2 cells. Next, we investigated endothelial cell-targeting therapies using glioma initiating cells (TSG). TSG cells highly expressed CXCR4. Under specific culture condition of the glioma-initiating cells the cell morphology including high expression of CXCL12. CXCL12/CXCR-4 blockage by AMD 3100 or siRNA markedly inhibited the cell proliferation in a concentration-dependent manner. In vivo model, CXCL12 knockdown by shRNA inhibited the tumor growth. These results indicate that CXCL12 of glioma initiating cells is one of key molecules to mediate tumor growth.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (12 results)

All 2013 2012 2011 2010 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (9 results)

  • [Journal Article] Glioma Immunotherapy With Combined Autologous Tumor Cell and Endothelial Cell Vaccine In Vivo2012

    • Author(s)
      Sakamoto N, Uemae Y, Ishikawa E, Takano S, Nakai K, Yamamoto T, Zaboronok A, Matsumura A
    • Journal Title

      Neurol Med Chir (Tokyo)

      Volume: 52 Pages: 194-201

    • NAID

      10030127755

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Glioma Immunotherapy With Combined Autologous Tumor Cell and Endothelial Cell Vaccine In Vivo2012

    • Author(s)
      Sakamoto N, Uemae Y, Ishikawa E, Takano S, Nakai K, Yamamoto T, Zaboronok A, Matsumura A.
    • Journal Title

      Neurologia medico-chirurgica

      Volume: 52 Issue: 4 Pages: 194-201

    • DOI

      10.2176/nmc.52.194

    • NAID

      10030127755

    • ISSN
      0470-8105, 1349-8029
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Glioma Immunotherapy With Combined Autologous Tumor Cell and Endothelial Cell Vaccine In Vivo2012

    • Author(s)
      Sakamoto N, Uemae Y, Ishikawa E, Takano S, Nakai K, Yamamoto T, Zaboronok A, Matsuinura A.
    • Journal Title

      Neurol Med Chir (Tokyo)

      Volume: (in press)

    • NAID

      10030127755

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 悪性神経膠腫瘍に対するSDF-1(CXCL-12)/CXCR-4 を標的とした新規治療の開発2013

    • Author(s)
      石川栄一、上前洋二、松田真秀、大須賀覚、 山本哲哉、高野晋吾、松村 明
    • Organizer
      第8回脳腫瘍の基礎シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2013-03-02
    • Related Report
      2012 Final Research Report
  • [Presentation] マウス Brain Tumor-initiating Cell-line (BTICs)を用いた分子標的療法の開発2012

    • Author(s)
      石川栄一、上前洋二、大須賀覚、松田真秀、坂本規影、高野晋吾、中井啓、山本哲哉、松村 明
    • Organizer
      第30回日本脳腫瘍学会学術集会
    • Place of Presentation
      広島
    • Year and Date
      2012-11-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 不活化ウイルス粒子による悪性脳腫瘍治療の開発-免疫療法としての側面2012

    • Author(s)
      松田真秀、小沼邦之、石川栄一、金田安史、松村明
    • Organizer
      第9回がんワクチン療法研究会(AsCaVaTh)学術集会
    • Place of Presentation
      つくば
    • Year and Date
      2012-11-17
    • Related Report
      2012 Final Research Report
  • [Presentation] CXCL12 Targeting Therapy for Glioma Using a Murine Brain Tumor-Initiating Cell-line2012

    • Author(s)
      Uemae Y, Ishikawa E, Osuka S,MatsudaM, Sakamoto N, Takano S, Nakai K, Yamamoto T, Saya H, Matsumura A
    • Organizer
      The 3rdLeading Graduate Schools International Conference
    • Place of Presentation
      Tsukuba
    • Year and Date
      2012-11-02
    • Related Report
      2012 Final Research Report
  • [Presentation] マウス Brain Tumor-initiating Cell-line (BTICs)を用いた分子標的療法の開発2012

    • Author(s)
      石川栄一
    • Organizer
      第30回日本脳腫瘍学会
    • Place of Presentation
      広島
    • Related Report
      2012 Annual Research Report
  • [Presentation] ウイルスエンベロープを用いたdrug delivery systemの開発2011

    • Author(s)
      松田真秀,金田安史,石川栄一,中井啓,山本哲哉,高野晋吾,松村 明
    • Organizer
      第70回日本脳神経外科学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2011-10-14
    • Related Report
      2012 Final Research Report
  • [Presentation] マウスグリオーマ皮下モデルを用いた同種血管内皮細胞ワクチン療法の研究2010

    • Author(s)
      石川栄一、坂本規彰、上前洋二、山本哲哉、高野晋吾、松村明
    • Organizer
      第28回脳腫瘍学会
    • Place of Presentation
      軽井沢
    • Year and Date
      2010-11-30
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] CXCL12 Targeting Therapy for Glioma Using a Murine Brain Tumor–Initiating Cell-line

    • Author(s)
      Uemae Y
    • Organizer
      The 3rd Leading Graduate Schools International Conference
    • Place of Presentation
      つくば
    • Related Report
      2012 Annual Research Report
  • [Presentation] 悪性神経膠腫瘍に対するSDF-1(CXCL-12)/CXCR-4を標的とした新規治療の開発

    • Author(s)
      石川栄一
    • Organizer
      第8回脳腫瘍の基礎シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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