Evaluation of RANKL reverse signaling as therapeutic targets for osteoporosis

• Project/Area Number: 22791364
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: The University of Tokyo
• Principal Investigator: HONMA Masashi 東京大学, 医学部附属病院, 助教 (60401072)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 骨・軟骨代謝学 / シグナル伝達 / 発現制御 / 生体分子 / 骨粗鬆症 / 骨芽細胞 / 破骨細胞 / 双方向シグナル / RANKL

Research Abstract

Microtubules are necessary for RANKL translocation to the osteoblastic cell surface and Rac1/Cdc42-IQGAP1-CLIP170 are indicted to be involved in the reconstruction of microtubules towards the stimulated area. Moreover, mTORC2 is indicated to be activated in response to RANK stimulation and regulate Racc1/Cdc42 activation.

Research Products

• [Journal Article] Rab27a and Rab27b are involved in stimulation-dependent RANKL release from secretory lysosomes in osteoblastic cells. (2011)
  • Author(s): Yoshiaki Kariya, Masashi Houma (equally 1st), Akiko Hanamura, Shigeki Aoki, Tadashi Ninomiya, Yuko Nakamichi, Nobuyuki Udagawa, Hiroshi Suzuki
  • Journal Title: Journal of Bone and Mineral Research Volume: Volume 26, Issue 4 Pages: 689-703
  • Peer Reviewed
• [Journal Article] Hiroshi Suzuki Rab27a and Rab27b are involved in stimulation-dependent RANKL release from secretory lysosomes in osteoblastic cells (2010)
  • Author(s): Yoshiaki Kariya, Masashi Honma, Akiko Hanamura, Shigeki Aoki, Tadashi Ninomiya, Yuko Nakamichi, Nobuyuki Udagawa
  • Journal Title: Journal of Bone and Mineral Research Volume: Vol.26Issue4 Pages: 689-703
  • Peer Reviewed
• [Presentation] RANKL骨芽細胞内挙動とRANKL逆シグナル (2011)
  • Author(s): 本間雅
  • Organizer: 第14回骨発生・再生研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-11-12
• [Presentation] RANKL骨芽細胞内挙動とRANKL逆シグナル (2011)
  • Author(s): 本間雅
  • Organizer: 第14回骨発生・再生研究会
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2011-11-12
• [Presentation] RANKL reverse signaling triggers RANKL translocation via mTORC2 activation (2011)
  • Author(s): Masashi Honma, Akiko Hanamura, Shigeki Aoki, Yoshiaki Kariya and Hiroshi Suzuki
  • Organizer: 3rd Joint Meeting of the European Calcified Tissue Society & the International Bone and Mineral Society Athens
  • Place of Presentation: Greece
  • Year and Date: 2011-05-10
• [Presentation] RANKL reverse signaling triggers RANKL translocation via mTORC2 activation (2011)
  • Author(s): Masashi Honma, Akiko Hanamura, Shigeki Aoki, Yoshiaki Kariya, Hiroshi Suzuki
  • Organizer: 3rd Joint Meeting of the European Calcified Tissue Society & the International Bone and Mineral Society
  • Place of Presentation: Athens, Greece
  • Year and Date: 2011-05-10
• [Presentation] RANKL細胞内トラフィックを制御する分子基盤 (2011)
  • Author(s): 本間雅
  • Organizer: 第88回日本生理学会大会・第116回日本解剖学会総会・全国学術集会・合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-30
• [Presentation] RANKL細胞内トラフィックの新規治療標的としての可能性 (2011)
  • Author(s): 本間雅
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-24
• [Presentation] RANKL細胞内トラフィックの生理的意義 (2010)
  • Author(s): 本間雅
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-22