Efficient derivation of osteoprogenitor cells from induced pluripotent stem cells for bone regeneration

• Project/Area Number: 22791379
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Kobe University
• Principal Investigator: LEE Sangyang 神戸大学, 医学部附属病院, 特定助教 (40533732)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 四肢機能再建学 / 再生医療 / iPS細胞 / 骨分化 / 骨再生 / 偽関節 / 骨折 / マウスiPS細胞 / 骨分化誘導 / 胚葉体

Research Abstract

The novel direct-plating method described here provides a significant technical advance over conventional methods of iPSC. induced osteoprogenitor cells by avoiding the embryonic body formation that often leads to heterogeneous and unpredictable osteogenic differentiation. Because of simplicity, our novel direct-plating method could be amenable to large-scale production of osteoprogenitor cells for bone regeneration.

Research Products

• [Presentation] Efficient deriva tion of osteoprogenitor cells from ind uced pluripotent stem cell for bone r egeneration (2012)
  • Author(s): Dogaki Y, Lee SY, Niikura T, Koh A, Koga T, Okumachi E, Nishida K, Kur oda R, Kurosaka, M.
  • Organizer: 1^<st> AO Trauma Asia Pa cific Scientific Congress
  • Place of Presentation: Hong Kong, Chinna
• [Presentation] Efficient differentiation and characterization of murine ind uced pluripotent stem cell-derived os teoprogenitor cells without embryoid body formation (2012)
  • Author(s): Dogaki Y, Lee SY, Niikura T, Koh A, Koga T, Okumachi E, Kakutani K, Hirata H, Nishida K, Kuroda R, Kurosaka M.
  • Organizer: 58^<th> Annual Meeting of Orthopaedic Research Society
  • Place of Presentation: San Francisco, CA, USA.
• [Presentation] Efficient Differentiation and Characterization of Murine Induced Pluripotent Stem Cell-Derived Osteoprogenitor Cells without Embryoid Body Formation (2012)
  • Author(s): 堂垣佳宏
  • Organizer: Annual meeting of Orthopaedic Research Society
  • Place of Presentation: Moscone West Convention Center, San Francisco, CA, USA