The effect of human mesenchymal stem cell and risedronate on TNF-alpha-induced osteoclast differentiation

• Project/Area Number: 22791384
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Okayama University
• Principal Investigator: FUJITA Hirofumi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (20423288)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 破骨細胞 / 間葉系幹細胞 / ビスホスフォネート / 顎骨壊死 / ビスフォスフォネート

Research Abstract

We investigated the effect of human bone marrow mesenchymal stem cell(hMSC) and bisphosphonate on inflammatory cytokine-induced osteoclast differentiation. Our findings showed that osteoclast differentiation by TNF-alpha was suppressed by hMSC and the suppression was inhibited by bisphosphonate.

Research Products

• [Journal Article] Serum-dependent export of protoporphyrin IX by ATP-binding cassette transporter G2 in T24 cells (2011)
  • Author(s): Ogino T, Kobuchi H, Munetomo K, Fujita H, Yamamoto M, Utsumi T, Inoue K, Shuin T, Sasaki J, Inoue M, Utsumi K
  • Journal Title: Mol Cell Biochem Volume: 358(1-2) Pages: 297-307
  • Peer Reviewed
• [Journal Article] Effect of risedronate on osteoblast differentiation, expression of receptor activator of NF-κB ligand and apoptosis in mesenchymal stem cells (2011)
  • Author(s): Fujita H, Kurokawa K, Ogino T, Ono M, Yamamoto M, Oka T, Nakanishi T, Kobayashi N, Tanaka N, Ogawa T, Suzaki E, Utsumi K, Sasaki J.
  • Journal Title: Basic Clin Pharmacol Toxicol. Volume: 109(2) Issue: 2 Pages: 78-84
  • DOI: 10.1111/j.1742-7843.2011.00685.x
  • Peer Reviewed
• [Journal Article] Effect of Risedronate on Osteoblast Differentiation, Expression of Receptor Activator of NF-κB Ligand and Apoptosis in Mesenchymal Stem Cells. (2011)
  • Author(s): Fujita H, et al
  • Journal Title: Basic Clin Pharmacol Toxicol Volume: (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] α-Tocopheryl succinate induces rapid and reversible phosphatidylserine externalization in histiocytic lymphoma through the caspase-independent pathway (2010)
  • Author(s): Fujita H, Shiva D, Utsumi T, Ogino T, Ogawa T, Abe K, Yasuda T, Utsumi K and Sasaki J
  • Journal Title: Mol Cell Biochem Volume: 333 Pages: 137-149
  • Peer Reviewed
• [Journal Article] ビタミンEサクシネートによるホスファチジルセリンの細胞表面転移の解析 (2010)
  • Author(s): 藤田洋史, ら
  • Journal Title: ビタミンE研究の進歩XIV Volume: XIV Pages: 13-20
• [Presentation] 炎症性サイトカインによる破骨細胞形成に対するビタミンE及びその誘導体の作用の解析 (2011)
  • Author(s): 藤田洋史
  • Organizer: 第14回Vitamin E Update Forum
  • Place of Presentation: 東京
  • Year and Date: 2011-08-30
• [Presentation] 骨髄間葉系幹細胞の骨芽細胞分化誘導に伴う細胞死と石灰化の機構解析 (2011)
  • Author(s): 藤田洋史, 内海耕慥, 佐々木順造
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] Effect ofα-tocopherol and its derivative in TNFα-induced osteoclastogenesis in vitro (2011)
  • Author(s): Hirofumi Fujita, Junzo Sasaki, et al
  • Organizer: The 5th Biennial Meeting of Society for Free Radical Research-Asia(SFRR-Asia)
  • Place of Presentation: Kagoshima
• [Presentation] In vitroにおける骨髄間葉系幹細胞の骨芽細胞分化に伴う石灰化には細胞死が関与する (2011)
  • Author(s): 藤田洋史, 斧美緒, 佐々木順造
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] 炎症性サイトカインによる破骨細胞形成機構とそれに対するビタミンE誘導体の影響 (2011)
  • Author(s): 藤田洋史, 佐々木順造
  • Organizer: 第64回日本酸化ストレス学会
  • Place of Presentation: 北海道
• [Presentation] Dead cells promote calcification of mesenchymal stem cell-derived osteoblasts in vitro (2011)
  • Author(s): Hirofumi Fujita, Junzo Sasaki, et al
  • Organizer: 第116回日本解剖学会総会・全国学術集会
  • Place of Presentation: 横浜
• [Presentation] 骨髄間葉系幹細胞による破骨細胞形成の抑制とビスホスフォネートによるその阻害機構の解析 (2011)
  • Author(s): 斧美緒、藤田洋史、山本昌直、久木田敏夫、井上啓史、内海耕慥、佐々木順造
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] Effect of α-tocopherol and its derivative in TNFα-induced osteoclastogenesis in vitro (2011)
  • Author(s): Hirofumi Fujita, Tetsuya Ogino, Mio Ono, Toshio Kukita, Kozo Utsumi, Junzo Sasaki
  • Organizer: The 5th Biennial Meeting of Society for Free Radical Research-Asia
  • Place of Presentation: Kagoshima
• [Presentation] 炎症性サイトカインによる破骨細胞分化とビタミンE及びその誘導体の作用 (2011)
  • Author(s): 藤田洋史, ら
  • Organizer: 第22回ビタミンE研究会
  • Place of Presentation: 京都
• [Presentation] 間葉系幹細胞の骨芽細胞分化に伴う石灰化における細胞死の関与 (2010)
  • Author(s): 藤田洋史, 佐々木順造
  • Organizer: 第33回日本分子生物学会年会,第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] INHIBITION OF ATP-BINDING CASSETTE TRANSPORTER G2 INCREASED MITOCHONDRIAL LOCALIZATION OF PPIX IN ALA TREATED CELLS AND ENHANCED THEIR APOPTOTIC CELLBY PHOTODYNAMIC TREATMENT (2010)
  • Author(s): Utsumi K., Fujita H., Sasaki J, et al
  • Organizer: OzBio2010 Combined Conference
  • Place of Presentation: Melbourne
• [Presentation] ヒト間葉系幹細胞による炎症性破骨細胞分化の制御とビスフォスフォネートの影響. The suppression of inflammatory stimuli-induced osteoclast differentiation by human mesenchymal stemcell and the action of bisphosphonate (2010)
  • Author(s): 斧美緒, 藤田洋史, 佐々木順造
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 東京
• [Presentation] 内海耕慥ら5-Aminolevulinic acid依存性protoporphyrin IX蓄積に於けるABC transporterの機能と光動力学作用 (2010)
  • Author(s): 藤田洋史, 佐々木順造
  • Organizer: 第63回日本酸化ストレス学会学術集会
  • Place of Presentation: 神奈川
• [Presentation] 炎症性サイトカイン誘導性破骨細胞分化へのヒト間葉系幹細胞の作用とそれに対するビスフォスフォネートの影響 (2010)
  • Author(s): 藤田洋史, ら
  • Organizer: 日本解剖学会第65回中国・四国支部学術集会
  • Place of Presentation: 呉
• [Presentation] ヒト間葉系幹細胞による炎症性破骨細胞分化の制御とビスフォスフォネートの影響 (2010)
  • Author(s): 斧美緒、藤田洋史, ら
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 東京
• [Patent(Industrial Property Rights)] ミトコンドリアの蛍光染色方法 (2010)
  • Inventor(s): 藤田洋史, ら
  • Industrial Property Rights Holder: 岡山大学, 等
  • Industrial Property Number: 2010-106490
  • Filing Date: 2010-05-06