| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Research Abstract |
In this study, we generated a novel transgenic mouse expressing ffLuc, a novel reporter gene, which could express both fluorescent and luminescent proteins(a variant of YFP and Luc2), under the control of the Bdkrb1 promoter(Bdkrb1-ffLuc). In this novel mouse, we made well-known neuropathic pain models. First, we performed the Seltzer model, partial sciatic nerve ligation model. At 2 weeks post-injury, a robust luminescent signal was detected on the injured side, whereas a mild signal was also detected on the contralateral side. Furthermore, the luminescent signal was shifted from ipsilateral to contralateral side at 4 weeks post-injury.
|
