• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Diagnostic markers of urothelial cancer based on DNA methylation analysis

Research Project

Project/Area Number 22791508
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionNara Medical University

Principal Investigator

CHIHARA Yoshitomo  奈良県立医科大学, 医学部, 講師 (40405395)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords腫瘍学 / 膀胱がん / DNAメチル化 / 尿中剥離細胞 / 診断 / エピゲネティクス / エピジェネティクス
Research Abstract

In this multi-center study, three independent sample sets were prepared. First, DNA methylation levels at CpG loci were measured in the training sets using the Illumina GoldenGate methylation assay to identify differentially methylated loci. Next, these methylated loci were validated by quantitative DNA methylation by PSQ, using another cohort of tissue samples. Lastly, methylation of these markers was analyzed in the independent urine samples(Urine validation set). ROC analysis was performed to evaluate the diagnostic accuracy of 12 selected markers. In the panel analysis, 12 loci showed remarkable alterations between urine from cancer patients and healthy urine, with 100% sensitivity and 100% specificity. Similarly, these markers showed favorable diagnostic accuracy for recurrence.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (5 results)

All 2011 2010

All Presentation (5 results)

  • [Presentation] Diagnostic indicator of urothelial cancer based on DNA methylation analysis2011

    • Author(s)
      Chihara Y, Kuniyasu H, Liang G, et al.
    • Organizer
      American Urological Association Annual meeting
    • Place of Presentation
      ワシントンDC
    • Year and Date
      2011-05-15
    • Related Report
      2011 Final Research Report
  • [Presentation] Diagnostic indicator of urothelial cancer based on DNA methylation analysis2011

    • Author(s)
      Chihara Yoshitomo
    • Organizer
      American Urological Assosiation Annual Meeting
    • Place of Presentation
      Washington DC
    • Year and Date
      2011-05-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] 尿中DNAメチル化異常を指標とした尿路上皮がん診断2011

    • Author(s)
      千原良友、金井弥栄、藤本博行
    • Organizer
      日本泌尿器科学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-04-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Two molecular pathways to bladder cancer involve opposing roles of DNA methylation2010

    • Author(s)
      千原良友
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2010-09-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Two molecular pathways to bladder cancer involve opposing roles of DNA methylation2010

    • Author(s)
      千原良友
    • Organizer
      日本癌学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2010-09-23
    • Related Report
      2011 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi