Research Project
Grant-in-Aid for Young Scientists (B)
We showed that SF-1 expression induces differentiation of mesenchymal stem cells into steroidogenic cells. In order to elucidate the mechanisms of epigenetic regulations in differentiation into steroidogenic cells including ovarian cells, we attempted a couple of experiments using the differentiated cell.(1) One is functional analysis of the nuclear complex components with SF-1.(2) The other is combined-analysis with ChIP-on-Chip and DNA microarray to identify novel SF-1 target genes.(1) Transcription factor C/EBPβwas identified as one of the nuclear complex components with SF-1. We found that C/EBPβcooperated with SF-1 to enhance the transcriptional activity of HSD3B2 gene expression, which codes for one of the enzymes for progesterone production.(2) We identified GSTA3, ALAS1 and FDXR as novel SF-1-target genes. Transcriptional activity of these genes were enhanced by SF-1 via epigenetic change. We also found these factors were involved in steroidogenesis. These results indicate that these factors are novel SF-1-target genes and participate in steroid hormone production.
All 2011 2010
All Journal Article (9 results) (of which Peer Reviewed: 7 results) Presentation (50 results) Patent(Industrial Property Rights) (2 results)
Genes & Genetic Systems
Volume: 86 Issue: 5 Pages: 305-314
10.1266/ggs.86.305
10030209843
日本生殖内分泌学会雑誌
Volume: 16 Pages: 27-29
40019009388
http://seishoku.org/11_16kan/09-topics1.pdf
生化学
Volume: 83(5) Pages: 388-391
10029068441
http://www.soc.nii.ac.jp/jbiochem/magazine/83-05-05.pdf
Mol. Cell. Endocrinol
Volume: 336 Issue: 1-2 Pages: 123-126
10.1016/j.mce.2010.11.031
Volume: 336 Issue: 1-2 Pages: 127-132
10.1016/j.mce.2010.11.025
Mol.Cell.Endocrinol.
Volume: 336 Pages: 123-126
Volume: 336 Pages: 127-132
J. Biol. Chem
Volume: 285(36) Issue: 36 Pages: 28240-28251
10.1074/jbc.m110.129510
J.Biol.Chem.
Volume: 285(36) Pages: 28240-28251
