Elucidation of the mechanisms of novel epigenetic regulations in ovarian differentiation
| Project/Area Number |
22791523
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 生殖医学 / ステロイドホルモン / SF-1 / ALAS1 / FDXR / GSTA3 / エピジェネティクス / 卵巣 / 分化 / 転写 / C/EBPβ |
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| Research Abstract |
We showed that SF-1 expression induces differentiation of mesenchymal stem cells into steroidogenic cells. In order to elucidate the mechanisms of epigenetic regulations in differentiation into steroidogenic cells including ovarian cells, we attempted a couple of experiments using the differentiated cell.(1) One is functional analysis of the nuclear complex components with SF-1.(2) The other is combined-analysis with ChIP-on-Chip and DNA microarray to identify novel SF-1 target genes.(1) Transcription factor C/EBPβwas identified as one of the nuclear complex components with SF-1. We found that C/EBPβcooperated with SF-1 to enhance the transcriptional activity of HSD3B2 gene expression, which codes for one of the enzymes for progesterone production.(2) We identified GSTA3, ALAS1 and FDXR as novel SF-1-target genes. Transcriptional activity of these genes were enhanced by SF-1 via epigenetic change. We also found these factors were involved in steroidogenesis. These results indicate that these factors are novel SF-1-target genes and participate in steroid hormone production.
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Report
(3 results)
Research Products
(61 results)
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[Journal Article] Fub1p, a novel protein isolated by boundary screening, binds the proteasome complex2011
Author(s)
Hatanaka A, Chen B, Sun J-Q, Mano Y, Funakoshi M, Kobayashi H, Ju Y, Mizutani T, Shimyozu K, Nakayama J, Miyamoto K, Uchida H, Oki M
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Journal Title
Genes & Genetic Systems
Volume: 86
Issue: 5
Pages: 305-314
DOI
NAID
ISSN
1341-7568, 1880-5779
Related Report
Peer Reviewed
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[Journal Article] Stem cell differentiation into steroidogenic cell lineages by NR5A family2011
Author(s)
Miyamoto, K., Yazawa, T., Mizutani, T., Imamichi, Y., Kawabe, S., Kanno, M., Matsumura, T., Ju, Y., Umezawa, A.
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Journal Title
Mol. Cell. Endocrinol
Volume: 336
Issue: 1-2
Pages: 123-126
DOI
Related Report
Peer Reviewed
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[Journal Article] Differentiation of mesenchymal stem cells and embryonic stem cells into steroidogenic cells using steroidogenic factor-1 and liver receptor homolog-12011
Author(s)
Yazawa, T., Kawabe, S., Inaoka, Y., Okada, R., Mizutani, T., Imamichi, Y., Ju, Y., Yamazaki, Y., Usami, Y., Kuribayashi, M., Umezawa, A., Miyamoto, K.
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Journal Title
Mol. Cell. Endocrinol
Volume: 336
Issue: 1-2
Pages: 127-132
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of a novel distal control region upstream of the human steroidogenic acute regulatory protein(StAR) gene that participates in SF-1-dependent chromatin architecture2010
Author(s)
Mizutani, T., Yazawa, T., Ju, Y., Imamichi, Y., Uesaka, M., Inaoka, Y., Matsuura, K., Kamiki, Y., Oki, M., Umezawa, A., Miyamoto, K.
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Journal Title
J. Biol. Chem
Volume: 285(36)
Issue: 36
Pages: 28240-28251
DOI
Related Report
Peer Reviewed
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[Presentation] Glutatione S-transferase A3(GSTA3)プロモーター領域における転写制御2011
Author(s)
松村健大,今道力敬,水谷哲也,具云峰,矢澤隆志,菅野真史,河邉真也,梅澤明弘,稲谷大,赤木好男,宮本薫
Organizer
第34回日本分子生物学会年会
Place of Presentation
横浜
Related Report
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[Presentation] Delta-aminolevulinate synthase 1(ALAS1) is a novel steroidogenic factor-1(SF-1) target gene important for steroidogenesis2011
Author(s)
Ju, Y., Mizuani, T., Imamichi, Y., Matsumura, T., Yazawa, T., Kawabe, S., Kanno, M., Umezawa, A., Miyamoto, K.
Organizer
第34回日本分子生物学会年会
Place of Presentation
横浜
Related Report
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[Presentation] SF-1によるクロマチン構造変換を介した新たな転写調節機構2011
Author(s)
水谷哲也,具云峰,今道力敬,松村健大,矢澤隆志,河邉真也,菅野真史,松浦かおる,上木康衣,梅澤明弘,尾崎司,南野直人,宮本薫
Organizer
第84回日本内分泌学会学術集会
Place of Presentation
神戸
Related Report
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[Presentation] 転写因子SF-1の新たな標的遺伝子の同定2011
Author(s)
今道力敬,水谷哲也,具云峰,松村健大,矢澤隆志,河邉真也,菅野真史,梅澤明弘,小亀浩一,寒川賢治,宮本薫
Organizer
第84回日本内分泌学会学術集会
Place of Presentation
神戸
Related Report
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[Presentation] Transcriptional regulation of steroidogenic-related genes by SF-1 through its dependent alternations of chromatin structure2011
Author(s)
Mizutani, T., Ju, Y., Imamichi, Y., Matsumura, T., Yazawa, T., Kawabe, S., Kanno, M., Osaki, T., Minamino, N., Umezawa, A., Miyamoto, K.
Organizer
Experimental Biology 2011
Place of Presentation
Washington, DC.
Related Report
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[Presentation] ヒトSt遺伝子の新たな転写調節機構2010
Author(s)
水谷哲也,矢澤隆志,具云峰,今道力敬,松村健大,河邉真也,松浦かおる,上木康衣,梅澤明弘,宮本薫
Organizer
日本生化学会北陸支部第28回大会
Place of Presentation
福井
Year and Date
2010-05-29
Related Report
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[Presentation] クロマチン構造変換を介したStARの新たな転写調節メカニズム2010
Author(s)
水谷哲也,具云峰,今道力敬,松村健大,矢澤隆志,河邉真也,菅野真史,松浦かおる,上木康衣,梅澤明弘,尾崎司,南野直人,宮本薫
Organizer
第83回日本生化学会大会・第33回日本分子生物学会年会BMB2010
Place of Presentation
神戸
Related Report
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[Presentation] PGC-1αはSF-1とLRH-1のコアクチベーターとしてプロジェステロン産生を促進する2010
Author(s)
矢澤隆志,稲岡斉彦,岡田令子,河邉真也,水谷哲也,今道力敬,山崎由希子,宇佐美陽子,栗林真悠,梅澤明弘,宮本薫
Organizer
第83回日本生化学会大会・第33回日本分子生物学会年会BMB2010
Place of Presentation
神戸
Related Report
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[Presentation] 卵巣・顆粒膜細胞におけるアンドロジェンの作用2010
Author(s)
矢澤隆志,河邉真也,稲岡斉彦,岡田令子,水谷哲也,今道力敬,山崎由希子,宇佐美陽子,栗林真悠,梅澤明弘,宮本薫
Organizer
日本動物学会第81回大会
Place of Presentation
東京
Related Report
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[Presentation] Regulation of SF-1-mediated transcription of the human steroidogenic acute regulatory protein gene by chromation-loop formation2010
Author(s)
Mizutani, T., Yazawa, T., Ju, Y., Uesaka, M., Inaoka, Y., Imamichi, Y., Matsuura, K., Kamiki, Y., Umezawa, A., Miyamoto, K.
Organizer
The 92th Annual Meeting & Expo
Place of Presentation
San Diego
Related Report
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