Genetic chemosensitivity test with novel drug-resistance genes in epithelial ovarian cancer

• Project/Area Number: 22791531
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Tottori University
• Principal Investigator: NANIWA Jun 鳥取大学, 医学部, 助教 (70467702)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 上皮性卵巣癌 / 薬剤耐性 / 抗癌剤感受性試験 / 感受性試験 / 耐性遺伝子 / 卵巣癌

Research Abstract

We established two ovarian cancer cell lines which showed drug resistance for Paclitaxel/Cisplatin from their parental cell lines. The cDNA microarray analysis of these parental and resistant cell proposed eight candidate genes(ABCB1, ABCG2, IL6, IL8, DR5, p21, KISS1, MMP1) for the novel chemo-sensitivity test, which levels in resistant cell were significantly higher than in parental cell. The result of quantitative analysis of the eight genes in these ovarian cancer cell lines supported this consequence. Gaining informed consent, 13 ovarian cancer clinical samples and information were provided, which is available for database. The quantitative analysis of these eight genes in these 13 clinical samples revealed that the levels of resistant group were higher than sensitive group.