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Tissue remodeling associated with endometrium and endometriosis

Research Project

Project/Area Number 22791536
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionOita University

Principal Investigator

TSUNO Akitoshi  大分大学, 医学部, 助教 (30457634)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords子宮内膜症 / collagen gell / 瘢痕化 / Fasudil / collagen gel / Fasudil dihydrochloride / 癒着・瘢痕化 / 細胞増殖抑制 / collagen gel三次元培養法
Research Abstract

Fasudil significantly inhibited the proliferation and contractility of ECSC and induced the cell cycle arrest in the G2/M phase and apoptosis of these cells. Morphological observation revealed the suppression of ECSC attachment to collagen fibers and decrease of cell density by fasudil. The expression of_-smooth muscle actin, RhoA, ROCK-I, and ROCK-II proteins was inhibited by fasudil administration. The expression of the antiapoptotic factors, Bcl-2 and Bcl-XL, in two-dimensional cultured ECSC were down-regulated by the addition of fasudil, whereas, the expression of p16INK4a and p21Waf1/Cip1 was up-regulated by the addition of fasudil.
The present findings suggest that fasudil is a promising agent for the treatment of endometriosis. The inhibition of cell proliferation, contractility, and myofibroblastic differentiation, the attenuation of attachment to collagen fibers, the decrease of cell density, and the induction of cell cycle arrest and apoptosis of ECSC are involved in the active mechanisms of fasudil.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (11 results)

All 2012 2011 2010

All Journal Article (7 results) (of which Peer Reviewed: 6 results) Presentation (4 results)

  • [Journal Article] Fasudil inhibits the proliferation and contractility and induces cell cycle arrest and apoptosis of human endometriotic stromal cells : A promising agent for the treatment of endometriosis2011

    • Author(s)
      Akitoshi Tsuno, Kaei Nasu, Yasushi Kawano, Akitoshi Yuge, Hairi Li, Wakana Abe, Hisashi Narahara
    • Journal Title

      J Clin Endocrinol Metab

      Volume: 96(12)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Attachment to extracellular matrices is enhanced in human endometriotic stromal cells : a possible mechanism involved in the pathogenesis of endometriosis2011

    • Author(s)
      Masatake Adachi, Kaei Nasu, Akitoshi Tsuno, Akitoshi Yuge, Yasushi Kawano, Hisashi Narahara
    • Journal Title

      Eur J Obstet Gynecol Reprod Biol

      Volume: 155 Pages: 85-88

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Application of the histone deacetylase inhibitors for the treatment of endometriosis : histone modifications as pathogenesis and novel therapeutic target2011

    • Author(s)
      Yulie Kawano, Kaei Nasu, Haili Li, Akitoshi Tsuno, Wakana Abe, Noriyuki Takai, Hisashi Narahara
    • Journal Title

      Human Reprod

      Volume: 26(9) Pages: 2486-2498

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aberrant expression of apoptosis-related molecules in endometriosis : a possible mechanism underlying the pathogenesis of endometriosis2011

    • Author(s)
      Kaei Nasu, Nishida Masakazu, Yasushi Kawano, Akitoshi Tsuno, Wakana Abe, Akitoshi Yuge, Noriyuki Takai, Hisashi Narahara
    • Journal Title

      Reprod Sci

      Volume: 18 Pages: 206-218

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 透析医療と他科連携-見逃しやすい疾患を中心に:婦人科【透析患者と月経異常・不正性器出血】2011

    • Author(s)
      津野晃寿、甲斐健太郎、河野康志、楢原久司
    • Journal Title

      臨牀透析

      Volume: Vol.27 No.2(10)

    • Related Report
      2011 Final Research Report
  • [Journal Article] Fasudil inhibits the proliferation and contractility and induces cell cycle arrest and apoptosis of human endometriotic stromal cells : A promising agent for the treatment of endometriosis2011

    • Author(s)
      Akitoshi Tsuno, Kaei Nasu, Yasushi Kawano, Akitoshi Yuge, Hairi Li, Wakana Abe, Hisashi
    • Journal Title

      J Clin Endocrinol Metab

      Volume: 96(12) Pages: 2516-2523

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of the histone deacetylase inhibitors for the treatment of endometriosis : histone modifications as pathogenesis and novel therapeutic target2011

    • Author(s)
      Yukie Kawano, Kaei Nasu, Haili Li, Akitoshi Tsuno, Wakana Abe, Noriyuki Takai, Hisashi Narahara
    • Journal Title

      Human Reprod

      Volume: 26 Pages: 2486-2498

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 子宮内膜症に対するFasudil dihydrochlorideの効果2012

    • Author(s)
      津野晃寿
    • Organizer
      エンドメトリオーシス学会
    • Place of Presentation
      ホテルニュー長崎
    • Year and Date
      2012-01-21
    • Related Report
      2011 Final Research Report
  • [Presentation] 子宮内膜症に対するFasudil dihydrochlorideの効果2012

    • Author(s)
      津野晃寿
    • Organizer
      エンドメトリオーシス学会
    • Place of Presentation
      長崎県
    • Year and Date
      2012-01-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] 子宮内膜症による瘢痕化に対するヘパリンの作用2011

    • Author(s)
      津野晃寿
    • Organizer
      第63回日本産科婦人科学会学術講演会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2011-08-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 子宮内膜症による瘢痕形成に対するFasudilの効果2010

    • Author(s)
      津野晃寿
    • Organizer
      第62回 日本産婦人科学会 学術講演会 総会
    • Place of Presentation
      東京
    • Year and Date
      2010-04-24
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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