Study of regulation of maternal T cell function[s] by placenta specific microRNAs

• Project/Area Number: 22791554
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Nippon Medical School
• Principal Investigator: ARI Mohamedo 日本医科大学, 医学部, 助教 (50573226)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 産科学 / 妊娠 / マイクロ RNA / 免疫細胞 / マイクロRNA / T細胞

Research Abstract

We hypothesized that circulating placenta-specific miRNAs could be delivered to maternal lymphocytes via exosomes and could be functional in the recipient cells. We tested this hypothesis using an in vitro model system; the human trophoblast cell line BeWo and T cell lymphoblast-like cell line Jurkat. We demonstrated the miRNAs present in BeWo-exosomes to be capable of modulating gene expression in Jurkat cells using in the in vitro model system. We provide a new insight into cell-cell communication via exosomes between placenta and lymphocytes.

Research Products

• [Journal Article] 二本鎖RNAセンサーによるリガンド認識とシグナル伝達機構の複雑性 (2012)
  • Author(s): 瀧澤俊広,石橋宰,アリモハメド
  • Journal Title: 実験医学 Volume: 30 Pages: 1303-1306
  • URL: https://www.yodosha.co.jp/jikkenigaku/current2012.html
• [Journal Article] Hydroxysteroid(17-β) dehydrogenase 1 is dysregulated by miR-210 and miR-518c that are aberrantly expressed in preeclamptic placentas : a novel marker for predicting preeclampsia (2012)
  • Author(s): Ishibashi O, Ohkuchi A, Ali MM, Kurashina R, Luo SS, Ishikawa T, Takizawa T, Hirashima C, Takahashi K, Migita M, Ishikawa G, Yoneyama K, Asakura H, Izumi A, Matsubara S, Takeshita T, Takizawa T
  • Journal Title: Hypertension Volume: 59 Issue: 2 Pages: 265-273
  • DOI: 10.1161/hypertensionaha.111.180232
  • Peer Reviewed
• [Journal Article] Short RNA duplexes elicit RIG-I-mediated apoptosis in a cell type-and length-dependent manner (2011)
  • Author(s): Ishibashi O, Ali MM, Luo SS, Ohba T, Katabuchi H, Takeshita T, Takizawa T
  • Journal Title: Sci Signal Volume: 4 Issue: 198 Pages: 74-74
  • DOI: 10.1126/scisignal.2001614
  • Peer Reviewed
• [Presentation] microRNAの網羅的解析手法と生殖免疫学分野での応用.シンポジウム3生殖医学研究に役立つニュー・コンセプト、ニュー・テクノロジー (2012)
  • Author(s): 菊池邦生,アリモハメド,岩城隼,吉武洋,瀧澤敬美,石川源,松原茂樹,竹下俊行,瀧澤俊広
  • Organizer: 第27回日本生殖免疫学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-12-09
• [Presentation] Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1) is dysregulated by miR-210 and miR-518c in the human placenta complicated with preeclampsia: plasma levels of HSD17B1 as a novel marker for predicting late-onset preeclampsia. (2012)
  • Author(s): Takizawa T, Ohkuchi A, Ali MM, Luo SS, Takizawa T, Migita M, Ishikawa G, Matsubara S, Takeshita T.
  • Organizer: IFPA 2012 Hiroshima Meeting(第18回国際胎盤学会)
  • Place of Presentation: 広島
  • Year and Date: 2012-09-19
• [Presentation] BeWo-cell-derived miR-517a modulates the expression of PRKG1 in Jurkat cells via Exosomes. (2012)
  • Author(s): Ali MM, Takizawa T, Kikuchi K, Saito S, Takizawa T.
  • Organizer: IFPA 2012Hiroshima Meeting(第18回国際胎盤学会)
  • Place of Presentation: 広島
  • Year and Date: 2012-09-18
• [Presentation] Placenta specificmiR-517a modulates gene expression in Jurkat cells. (2012)
  • Author(s): Ali MM, Song X, Ishibashi O, Kikuchi K, Ishikawa T, Takizawa T, Takizawa T.
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨
  • Year and Date: 2012-03-26
• [Presentation] Placenta specific miR-517α modulates gene expression in Jurkat cells (2012)
  • Author(s): アリモハメド
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス(山梨県)
  • Year and Date: 2012-03-26
• [Presentation] BeWo-cell-derived miR-517a modulates the expression of PRKG1 in Jurkat cells via Exosomes (2012)
  • Author(s): アリ モハメド
  • Organizer: IFPA 2012 Hiroshima Meeting（第18回国際胎盤学会）
  • Place of Presentation: 広島国際会議場（広島）
• [Presentation] Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1) is dysregulated by miR-210 and miR-518c in the human placenta complicated with preeclampsia: plasma levels of HSD17B1 as a novel marker for predicting late-onset preeclampsia (2012)
  • Author(s): 瀧澤 俊広
  • Organizer: IFPA 2012 Hiroshima Meeting（第18回国際胎盤学会）（招待講演）
  • Place of Presentation: 広島国際会議場（広島）
  • Invited
• [Presentation] microRNAの網羅的解析手法と生殖免疫学分野での応用 (2012)
  • Author(s): 菊池 邦生
  • Organizer: 第27回日本生殖免疫学会総会・学術集会
  • Place of Presentation: 大阪医科大学（大阪）
• [Presentation] クソソームmicroRNAを介した胎盤-リンパ球間コミュニケーション:培養細胞を用いたモデル解析 (2011)
  • Author(s): アリモハメド,菊池邦生,石橋宰,石川朋子,瀧澤敬美,竹下俊行,瀧澤俊広
  • Organizer: 第26回日本生殖免疫学会総会・学術集
  • Place of Presentation: 愛知
  • Year and Date: 2011-12-03
• [Presentation] エクソソームmicroRNAを介した胎盤-リンパ球間コミュニケーション:培養細胞を用いたモデル解析 (2011)
  • Author(s): アリモハメド
  • Organizer: 第27回日本生殖免疫学会総会・学術集会
  • Place of Presentation: ウィンク愛知(愛知県)
  • Year and Date: 2011-12-03
• [Presentation] 胎盤特異的micro RNA (2011)
  • Author(s): 瀧澤俊広,石橋宰,菊池邦生,アリモハメド,石川朋子,瀧澤敬美,大口昭英,松原茂樹,竹下俊行
  • Organizer: 第56回日本人類遺伝学会
  • Place of Presentation: 千葉
  • Year and Date: 2011-11-10
• [Presentation] 胎盤特異的microRNA (2011)
  • Author(s): 瀧澤俊広
  • Organizer: 56回日本人類遺伝学会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2011-11-10
• [Presentation] 胎盤由来エクソソームを介した胎盤-母体間コミュニケーション (2011)
  • Author(s): 菊池邦生,アリモハメド,羅善順,石橋宰,石川朋子,瀧澤敬美,倉品隆平,石川源,右田真,大口昭英,松原茂樹,竹下俊行,瀧澤俊広
  • Organizer: 第19回日本胎盤学会学術集会/第29回日本絨毛性疾患研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-01
• [Presentation] 胎盤由来エクソソームを介した胎盤-母体間コミュニケーション (2011)
  • Author(s): 菊池邦生
  • Organizer: 第19回日本胎盤学会学術集会
  • Place of Presentation: 東京ステーションコンファレンス(東京都)
  • Year and Date: 2011-10-01
• [Presentation] 胎盤由来のエクソソーム:胎盤特異的microRNAはエクソソームを介して母体循環に分泌される (2011)
  • Author(s): 瀧澤俊広,アリハメド,羅善順,石橋宰,菊池邦生,石川朋子,瀧澤敬美,倉品隆平,右田真,大口昭英,松原茂樹,竹下俊行
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] 胎盤由来のエクソソーム:胎盤特異的microRNAはエクソソームを介して母体循環に分泌される (2011)
  • Author(s): 瀧澤俊広
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-09-22
• [Presentation] BeWo exosome-associated microRNAs (miRNAs) are transferable and capable of modulating gene expression in Jurkat cells : miRNA-communication between placenta and T cells via exosomes (2011)
  • Author(s): Md.Moksed Ali
  • Organizer: 第116回日本解剖学会
  • Place of Presentation: 神奈川
  • Year and Date: 2011-03-30
• [Presentation] Placenta specific-microRNAs in normal pregnancy and preeclampsia. (2010)
  • Author(s): Takizawa T,Ali MM,Ishibashi O, Kikuchi K,Kosuge T,Matsubara S, Takeshita T
  • Organizer: 8th European Congress on Reproductive Immunology. 第8回欧州生殖免疫学会
  • Place of Presentation: Munich, Germany
  • Year and Date: 2010-11-13
• [Presentation] Placenta specific-microRNAs in normal pregnancy and preeclampsia (2010)
  • Author(s): Toshihiro Takizawa
  • Organizer: 8th European Congress on Reproductive Immunology
  • Place of Presentation: Munich, Germany
  • Year and Date: 2010-11-13
• [Presentation] 妊娠高血圧症候群に関連するマイクロRNAの同定とその標的遺伝子 (2010)
  • Author(s): 石橋宰,アリモハメド,石川源,大口昭英,泉章夫,松原茂樹,米山剛一,朝倉啓文,竹下俊行,瀧澤俊広
  • Organizer: 第18回日本胎盤学会学術集会
  • Place of Presentation: 熊本
  • Year and Date: 2010-09-30
• [Presentation] BeWo exosome-associated microRNAs(miRNAs)are transferable and capable of modulating gene expression in Jurkat cells:miRNA-communication between placenta and T cells via exosomes.
  • Author(s): Ali MM,Ishibashi O,Ishikawa T, Kikuchi K,Takizawa T,Goto T,Takizawa T.
  • Organizer: 第116回日本解剖学会総会・全国学術集会.(誌上発表)