To investigate molecular mechanisms for dynamics of retinal angiogenesis using time-lapse imaging

• Project/Area Number: 22791654
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Kyoto University
• Principal Investigator: MURAKAMI Tomoaki 京都大学, 医学研究科, 助教 (50549095)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 眼生化学 / 分子生物学 / 網膜血管新生 / タイムラプス観察 / VEGF / アクチン / glycogen synthase kinase(GSK)-3β / stromal-derived factor(SDF)-1 / glycogen synthase kinase (GSK)-3β / stromal-derived factor (SDF)-1

Research Abstract

We established a novel time-lapse imaging for retinal angiogenesis in retinal explants. The quantification elucidated both dynamic and static phases in angiogenic sprouting. Further, we demonstrated a novel finding that SDF-1 and GSK-3βregulate the motility of vascular endothelial cells in retinal angiogenesis.

Research Products

• [Journal Article] SDF-1/CXCR4contributes to the activation of tip cells and microglia in retinal angiogenesis (2010)
  • Author(s): Unoki N, Murakami T, Nishijima K, Ogino K, van Rooijen N, Yoshimura N
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 51(7) Issue: 7 Pages: 3362-71
  • DOI: 10.1167/iovs.09-4978
  • Peer Reviewed
• [Journal Article] Time-lapse imaging of retinal angiogenesis reveals decreased development and progression of neovascular sprouting byanecortave desacetate (2010)
  • Author(s): Unoki N, Murakami T, Ogino K, Nukada M, Yoshimura N
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 51(5) Issue: 5 Pages: 2347-55
  • DOI: 10.1167/iovs.09-4158
  • Peer Reviewed
• [Journal Article] SDF-1/CXCR4 contributes to the activation of tip cells and microglia in retinal angiogenesis (2010)
  • Author(s): Noriyuki Unoki, 他
  • Journal Title: Investigative Ophthalmology and Visual Science Volume: 51 Pages: 3362-3371
  • Peer Reviewed
• [Journal Article] Time-lapse imaging of retinal angiogenesis reveals decreased development and progression of neovascular sprouting by anecortave desacetate. (2010)
  • Author(s): Noriyuki Unoki, 他
  • Journal Title: Investigative Ophthalmology and Visual Science Volume: 51 Pages: 2347-2355
  • Peer Reviewed
• [Presentation] Annex in Vによる網膜血管新生と血管透過性に対する効果の検討 (2011)
  • Author(s): 諸岡諭(京都大学), 額田正之, 荻野顕, 村上智昭, 吉村長久
  • Organizer: 第115回日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-13
• [Presentation] Glycogen Synthase Kinase-3βInhibitors Reduce Vegf-induced Neovascular Sprouts In Retinal Explants (2011)
  • Author(s): Satoshi Morooka, Ken Ogino, Masayuki Nukada, Tomoaki Murakami, Nagahisa Yoshimura
  • Organizer: Association for Research in Vision and Ophthalmology(ARVO) annual meeting
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2011-05-04
• [Presentation] Glycogen Synthase Kinase-3β Inhibitors Reduce Vegf-induced Neovascular Sprouts In Retinal Explants (2011)
  • Author(s): Satoshi Morooka, 他
  • Organizer: 2011 Association for Research in Vision and Ophthalmology annual meeting
  • Place of Presentation: Fort Lauderdale/USA
  • Year and Date: 2011-05-04
• [Presentation] Annexin V Suppresses Pathological Retinal Angiogenesis And Disrupts Blood-retinal Barrier (2011)
  • Author(s): Masayuki Nukada, Tomoaki Murakami, Satoshi Morooka, Ken Ogino, Nagahisa Yoshimura
  • Organizer: Association for Research in Vision and Ophthalmology(ARVO) annual meeting
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2011-05-03
• [Presentation] Annexin V Suppresses Pathological Retinal Angiogenesis And Disrupts Blood-retinal Barrier (2011)
  • Author(s): Masayuki Nukada, 他
  • Organizer: 2011 Association for Research in Vision and Ophthalmology annual meeting
  • Place of Presentation: Fort Lauderdale/USA
  • Year and Date: 2011-05-03
• [Presentation] Stromal Cell-Derived Factor-1(SDF-1)/CXCR4 Promotes the Activation of Tip Cells and Microglia in Retinal Angiogenesis (2010)
  • Author(s): Unoki N, Murakami T, Nishijima K, Yoshimura N
  • Organizer: Association for Research in Vision and Ophthalmology(ARVO) annual meeting
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2010-05-02
• [Presentation] Time-Lapse Imaging of Retinal Angiogenesis Demonstrates That Anecortave Desacetate Attenuates Both the Development and Progression of Neovascular Sprouting (2010)
  • Author(s): Nukada M, Unoki N, Ogino K, Murakami T, Yoshimura N
  • Organizer: Association for Research in Vision and Ophthalmology(ARVO) annual meeting
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2010-05-02
• [Presentation] SDF-1/CXCR4 promotes the activation of tip cells and microglia in retinal angiogenesis (2010)
  • Author(s): Noriyuki Unoki, 他
  • Organizer: 2010 Association for Research in Vision and Ophthalmology annual meeting
  • Place of Presentation: Fort Lauderdale/U.S.A
  • Year and Date: 2010-05-02
• [Presentation] Time-lapse imaging of retinal angiogenesis demonstrates that anecortave desacetate attenuates both the development and progression of neovascular sprouting. (2010)
  • Author(s): Masayuki Nukada, 他
  • Organizer: 2010 Association for Research in Vision and Ophthalmology annual meeting
  • Place of Presentation: Fort Lauderdale/U.S.A
  • Year and Date: 2010-05-02
• [Patent(Industrial Property Rights)] 網膜新生血管に対する薬物効果の新規評価システム (2012)
  • Inventor(s): 村上智昭、吉村長久
  • Industrial Property Rights Holder: 村上智昭、吉村長久
  • Industrial Property Number: 2005-360983
  • Acquisition Date: 2012-02-21
  • Overseas