The evaluation of the marker genes during the development of trabecular meshwork cells and the induction of trabecular meshwork cells from human iPS cells

• Project/Area Number: 22791678
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: UENO Morio 京都府立医科大学, 医学研究科, 助教 (40426531)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 眼発生 / 再生医学 / 再生医療

Research Abstract

Transplantation of trabecular meshwork cells to the damaged trabecular meshwork tissues in patients of primary open angle glaucoma because one of causes of primary open angle glaucoma is the decrement of trabecular meshwork cells. In this study, we performed the development of the induction system of human trabecular meshwork cells from human induced pluripotent stem cells to mimic the in vivo development of trabecular meshwork tissues.

Research Products

• [Journal Article] Common variants in CDKN2B-AS1 associated with optic-nerve vulnerability of glaucoma identified by genome-wide association studies in Japanese (2012)
  • Author(s): Nakano M, Ikeda Y, Tokuda Y, Fuwa M, Omi N, Ueno M, Imai K, Adachi H, Kageyama M, Mori K, Kinoshita S, Tashiro K
  • Journal Title: PLoS One Volume: 7(3)
• [Journal Article] Enhancement of corneal endothelium wound healing by Rho-associated kinase (ROCK) inhibitor eye drops (2012)
  • Author(s): Okumura N, Koizumi N, Ueno M, Sakamoto Y, Takahashi H, Hirata K, Torii R, Hamuro J, Kinoshita S
  • Journal Title: Br J Ophthalmol Volume: 95 Issue: 7 Pages: 1006-9
  • DOI: 10.1136/bjo.2010.194571
  • Peer Reviewed
• [Journal Article] Enhancement of corneal endothelium wound healing by Rho-associated kinase(ROCK) inhibitor eye drops (2011)
  • Author(s): Okumura N, Koizumi N, Ueno M, Sakamoto Y, Takahashi H, Hirata K, Torii R, Hamuro J, Kinoshita S
  • Journal Title: Br J Ophthalmol Volume: 95(7) Pages: 1006-9
• [Journal Article] The new therapeutic concept of using a rho kinase inhibitor for the treatment of corneal endothelial dysfunction (2011)
  • Author(s): Okumura N, Koizumi N, Ueno M, Sakamoto Y, Takahashi H, Hamuro J, Kinoshita S
  • Journal Title: Cornea
• [Journal Article] Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells (2010)
  • Author(s): Ohgushi M, Matsumura M, Eiraku M, Murakami K, Aramaki T, Nishiyama A, Muguruma K, Nakano T, Suga H, Ueno M, Ishizaki T, Suemori H, Narumiya S, Niwa H, Sasai Y
  • Journal Title: Cell Stem Cell Volume: 7(2) Pages: 225-39
• [Journal Article] Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells (2010)
  • Author(s): Ohgushi M, Matsumura M, Eiraku M, Murakami K, Aramaki T, Nishiyama A, Muguruma K, Nakano T, Suga H, Ueno M, Ishizaki T, Suemori H, Narumiya S, Niwa H, Sasai Y.
  • Journal Title: Cell Stem Cell Volume: 7 Pages: 225-239
  • Peer Reviewed
• [Presentation] Induction of ocular tissues from human-induced pluripotent stem cells on the amniotic membrane matrix (2011)
  • Author(s): Ueno M, Nakai Y, Matsumura M, Sasai Y, Kinoshita S
  • Organizer: 2011 annual meeting of the ARVO
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2011-05-03
• [Presentation] Induction Of Ocular Tissues From Human-induced Pluripotent Stem Cells On The Amniotic Membrane Matrix (2011)
  • Author(s): Ueno M, Nakai Y, Matsumura M, Sasai Y, Kinoshita S
  • Organizer: ARVO Annual Meeting 2011
  • Place of Presentation: Fort Lauderdale, U.S.A.
  • Year and Date: 2011-05-03
• [Presentation] Induction of corneal epithelial cells from human embryonic stem cells on the amniotic membrane matrix (2011)
  • Author(s): Nakai Y, Ueno M, Matsumura M, Sasai Y, Kinoshita S
  • Organizer: 2011 annual meeting of the ARVO
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2011-05-01
• [Presentation] Induction Of Corneal Epithelial Cells From Human Embryonic Stem Cells On The Amniotic Membrane Matrix (2011)
  • Author(s): Nakai Y, Ueno M, Matsumura M, Sasai Y, Kinoshita S
  • Organizer: ARVO Annual Meeting 2011
  • Place of Presentation: Fort Lauderdale, U.S.A.
  • Year and Date: 2011-05-01
• [Presentation] 選択的Rhoキナーゼ阻害剤による角膜内皮細胞の細胞周期に対する影響 (2011)
  • Author(s): 奥村直毅, 小泉範子, 山崎健太, 上野盛夫, 坂本雄二, 曽和義広, 酒井敏行, 羽室淳爾, 木下茂
  • Organizer: 角膜カンファレンス2011
  • Place of Presentation: 東京
  • Year and Date: 2011-02-19
• [Presentation] 選択的Rhoキナーゼ阻害剤の角膜実質創傷治癒への影響 (2011)
  • Author(s): 山本真弓, 奥村直毅, 上野盛夫, 坂本雄二, 木下茂, Robert Young, Andrew Quantock, 小泉範子
  • Organizer: 角膜カンファレンス2011
  • Place of Presentation: 東京
  • Year and Date: 2011-02-17
• [Presentation] 京滋地区における過去3年間の緑内障薬物治療の変化の検討 (2010)
  • Author(s): 上野盛夫, 池田陽子, 今井浩二郎, 森和彦, 木下茂
  • Organizer: 第64回日本臨床眼科学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-11-13
• [Presentation] 緑内障検診における正常者乳頭形状グレード別判定結果 (2010)
  • Author(s): 池田陽子, 上野盛夫, 今井浩二郎, 森和彦, 木下茂
  • Organizer: 第64回日本臨床眼科学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-11-13
• [Presentation] 小眼球を伴うUveal effusionに対するマイトマイシンC併用強膜開窓術 (2010)
  • Author(s): 小森秀樹, 上野盛夫, 米田一仁, 木下茂
  • Organizer: 第64回日本臨床眼科学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-11-13
• [Presentation] 4歳で発症したVogt-小柳-原田病の1例 (2010)
  • Author(s): 永田健児, 上野盛夫, 小森秀樹, 多田玲, 森和彦, 中野由起子, 丸山和一, 木下茂
  • Organizer: 第64回日本臨床眼科学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-11-13
• [Presentation] Neural induction fromembryonic stem cells (2010)
  • Author(s): Ueno M
  • Organizer: Cornea to cortex research meeting
  • Place of Presentation: Cardiff
  • Year and Date: 2010-10-20
• [Presentation] Neural induction from embryonic stem cells (2010)
  • Author(s): Ueno M
  • Organizer: 9th EGS Congress
  • Place of Presentation: 英国Cardiff
  • Year and Date: 2010-10-20
• [Presentation] カスタムチップによる既知緑内障遺伝子解析 (2010)
  • Author(s): 池田陽子, 森和彦, 上野盛夫, 今井浩二郎, 大見奈津江, 不破正博, 中野正和, 八木知人, 徳田雄市, 田代啓, 木下茂
  • Organizer: 第21回日本緑内障学会
  • Place of Presentation: 博多
  • Year and Date: 2010-09-25
• [Presentation] 血液生化学データによる正常眼圧緑内障と全身疾患との関連性の検討 (2010)
  • Author(s): 今井浩二郎, 森和彦, 池田陽子, 上野盛夫, 木村健一, 木下茂
  • Organizer: 第21回日本緑内障学会
  • Place of Presentation: 博多
  • Year and Date: 2010-09-24
• [Presentation] Soemmering ringによる続発閉塞隅角緑内障眼に対する手術療法 (2010)
  • Author(s): 森和彦, 新開陽一郎, 加藤浩晃, 多田香織, 池田陽子, 上野盛夫, 今井浩二郎, 木下茂
  • Organizer: 第21回日本緑内障学会
  • Place of Presentation: 博多
  • Year and Date: 2010-09-24
• [Presentation] 網膜神経線維層厚解析装置による正常眼圧緑内障の長期経過観察 (2010)
  • Author(s): 高橋佳奈子, 池田陽子, 森和彦, 上野盛夫, 今井浩二郎, 岩間亜矢子, 多田香織, 吉村彰紘, 木下茂
  • Organizer: 第21回日本緑内障学会
  • Place of Presentation: 博多
  • Year and Date: 2010-09-24
• [Presentation] 正常眼圧緑内障の視神経乳頭形状解析装置による長期経過観察症例の検討 (2010)
  • Author(s): 吉村彰紘, 池田陽子, 森和彦, 上野盛夫, 今井浩二郎, 岩間亜矢子, 多田香織, 高橋佳奈子, 木下茂
  • Organizer: 第21回日本緑内障学会
  • Place of Presentation: 博多
  • Year and Date: 2010-09-24
• [Presentation] 多剤併用例におけるラタノプロストからビマトプロストへの切替え効果の検討 (2010)
  • Author(s): 南泰明, 池田陽子, 森和彦, 上野盛夫, 今井浩二郎, 岩間亜矢子, 多田香織, 高橋佳奈子, 木下茂
  • Organizer: 第21回日本緑内障学会
  • Place of Presentation: 博多
  • Year and Date: 2010-09-24
• [Presentation] ラタノプロスト点眼液及び安定性改善処方製剤MPR-0717の有効性比較試験 (2010)
  • Author(s): 高橋純子, 池田陽子, 森和彦, 上野盛夫, 今井浩二郎, 永田真帆, 木下茂
  • Organizer: 第21回日本緑内障学会
  • Place of Presentation: 博多
  • Year and Date: 2010-09-24
• [Presentation] 白内障手術後に生じた遅発性水晶体起因性続発緑内障 (2010)
  • Author(s): 多田香織, 上野盛夫, 森和彦, 池田陽子, 今井浩二郎, 木下茂
  • Organizer: 第21回日本緑内障学会
  • Place of Presentation: 博多
  • Year and Date: 2010-09-24
• [Presentation] Clinical-based observational study of glaucoma patient distribution and drug preference in Japanese common clinic in 2009 (2010)
  • Author(s): Ueno M, Ikeda Y, Imai K, Mori K, Kinoshita S
  • Organizer: 9th EGS Congress
  • Place of Presentation: スペインMadrid
  • Year and Date: 2010-09-12
• [Presentation] Long-term clinical outcomes of trabeculotomy ab externo for the treatment of glaucoma after corneal transplantation (2010)
  • Author(s): Mori K, Ikeda Y, Ueno M, Imai K, Kinoshita S
  • Organizer: 9th EGS Congress
  • Place of Presentation: スペインMadrid
  • Year and Date: 2010-09-12
• [Presentation] Three cases of lens-induced secondary glaucoma with combination mechanism which developed several years after cataract surgery (2010)
  • Author(s): Tada K, Ueno M, Mori K, Ikeda Y, Imai K, Kinoshita S
  • Organizer: Cornea to Cortex Research Seminar
  • Place of Presentation: スペインMadrid
  • Year and Date: 2010-09-12
• [Presentation] The effect of a ROCK inhibitor on corneal wound and the transformation of keratocytes (2010)
  • Author(s): Yamamoto M, Okumura N, Takahashi H, Ueno M, Sakamoto Y, Liles M, Young RD, Quantock AJ, Kinoshita S, Koizumi N
  • Organizer: BSMB autumn meeting 2010
  • Place of Presentation: Norwich
  • Year and Date: 2010-09-06
• [Presentation] The Effect of ROCK inhibitor on Corneal Wound Healing and the Trans formation of Keratocytes (2010)
  • Author(s): Yamamoto M, Okumura N, Takahashi H, Ueno M, Sakamoto Y, Liles M, Young RD, Quantock AJ, Kinoshita S, Koizumi N
  • Organizer: BSMB Autumn Meeting 2010
  • Place of Presentation: 英国Norwich
  • Year and Date: 2010-09-06
• [Presentation] Cell injection therapy using cultivated corneal endothelial cells combined with a ROCK inhibitor in a rabbit model (2010)
  • Author(s): Koizumi N, Okumura N, Takahashi H, Ueno M, Sakamoto Y, Hirata K, Hamuro J, Kinoshita S
  • Organizer: 2010 annual meeting of the ARVO
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2010-05-05
• [Presentation] The Rs16958477 SNP in the promoter region of the LOXL1 gene is associated with the LOXL1 gene expression level (2010)
  • Author(s): Imai K, Mori K, Ueno M, Ikeda Y, Kawasaki S Yagi T, Omi N, Fuwa M, Tashiro K, Kinoshita S
  • Organizer: 2010 annual meeting of the ARVO
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2010-05-05
• [Presentation] Cell-Injection Therapy Using Cultivated Corneal Endothelial Cells Combined With a ROCK Inhibitor in a Rabbit Model (2010)
  • Author(s): Koizumi N, Okumura N, Takahashi H, Ueno M, Sakamoto Y, Hirata K, Hamuro J, Kinoshita S
  • Organizer: 2010 Annual Meeting of the ARVO (The Association for Research in Vision and Ophthalmology)
  • Place of Presentation: アメリカ合衆国Fort Lauderdale
  • Year and Date: 2010-05-05
• [Presentation] The Rs16958477 SNP in the Promoter Region of the LOXL1 Gene is Associated With the LOXL1 Gene Expression Level (2010)
  • Author(s): Imai K, Mori K, Ueno M, Ikeda Y, Kawasaki S, Yagi T, Ohmi N, Fuwa M, Tashiro K, Kinoshita S
  • Organizer: 2010 Annual Meeting of the ARVO (The Association for Research in Vision and Ophthalmology)
  • Place of Presentation: アメリカ合衆国Fort Lauderdale
  • Year and Date: 2010-05-05
• [Presentation] Induction of ocular tissues from human pluripotent stem cells on the amniotic membrane matrix (2010)
  • Author(s): Ueno M, Nakai Y, Matsumura M, Takahashi M, Sasai Y, Kinoshita S
  • Organizer: 2010 annual meeting of the ARVO
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2010-05-04
• [Presentation] Choroidal thickness evaluation accompanied by intraocular pressure change using enhanced depth imaging optical coherence tomography (2010)
  • Author(s): Mori K, Tanaka H, Koizumi H, Ueno M, Ikeda Y, Imai K, Kinoshita S
  • Organizer: 2010 annual meeting of the ARVO
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2010-05-04
• [Presentation] The efficacy of a ROCK inhibitor containing corneal storage medium for corneal endothelial cells (2010)
  • Author(s): Takahashi H, Koizumi N, Okumura N, Ueno M, Sakamoto Y, Hamuro J, Kinoshita S
  • Organizer: 2010 annual meeting of the ARVO
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2010-05-04
• [Presentation] The effect of a ROCK inhibitor on corneal endothelial cell migration in an vitro wound-healing model (2010)
  • Author(s): Okumura N, Koizumi N, Ueno M, Sakamoto Y, Takahashi H, Hirata K, Hamuro J, Kinoshita S
  • Organizer: 2010 annual meeting of the ARVO
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2010-05-04
• [Presentation] Induction of Ocular Tissues From Human Pluripotent Stem Cells on the Amniotic Membrane Matrix (2010)
  • Author(s): Ueno M, Nakai Y, Matsumura M, Takahashi M, Sasai Y, Kisnoshita S
  • Organizer: 2010 Annual Meeting of the ARVO (The Association for Research in Vision and Ophthalmology)
  • Place of Presentation: アメリカ合衆国Fort Lauderdale
  • Year and Date: 2010-05-04
• [Presentation] Choroidal Thickness Evaluation Accompanied by Intraocular Pressure Change Using Enhanced Depth Imaging Optical Coherence Tomography (2010)
  • Author(s): Mori K, Tanaka H, Koizumi H, Ueno M, Ikeda Y, Imai K, Kinoshita S
  • Organizer: 2010 Annual Meeting of the ARVO (The Association for Research in Vision and Ophthalmology)
  • Place of Presentation: アメリカ合衆国Fort Lauderdale
  • Year and Date: 2010-05-04
• [Presentation] The Efficacy of A ROCK Inhibitor Containing Corneal Storage Medium for Corneal Endothelial Cells (2010)
  • Author(s): Takahashi H, Koizumi N, Okumura N, Ueno M, Sakamoto Y, Hamuro J, Kinoshita S
  • Organizer: 2010 Annual Meeting of the ARVO (The Association for Research in Vision and Ophthalmology)
  • Place of Presentation: アメリカ合衆国Fort Lauderdale
  • Year and Date: 2010-05-04
• [Presentation] The Effect of a ROCK Inhibitor on Corneal Endothelial Cell Migration in an in vitro Wound-Healing Model (2010)
  • Author(s): Okumura N, Koizumi N, Ueno M, Sakamoto Y, Takahashi H, Hirata K, Hamuro J, Kinoshita S.
  • Organizer: 2010 Annual Meeting of the ARVO (The Association for Research in Vision and Ophthalmology)
  • Place of Presentation: アメリカ合衆国Fort Lauderdale
  • Year and Date: 2010-05-04
• [Presentation] Association between general systemic disease and the marker SNPs for primary open angle glaucoma (2010)
  • Author(s): Ikeda Y, Mori K, Ueno M, Imai K, Yagi T, Omi N, Tokuda Y, Fuwa M, Tashiro K, Kinoshita S
  • Organizer: 2010 annual meeting of the ARVO
  • Place of Presentation: Fort Lauderdale
  • Year and Date: 2010-05-03
• [Presentation] Association Between General Systematic Disease and the Marker Snps for Primary Open-Angle Glaucoma (2010)
  • Author(s): Ikeda Y, Mori K, Ueno M, Imai K, Yagi T, Omi N, Tokuda Y, Fuwa M, Tashiro K, Kinoshita S.
  • Organizer: 2010 Annual Meeting of the ARVO (The Association for Research in Vision and Ophthalmology)
  • Place of Presentation: アメリカ合衆国Fort Lauderdale
  • Year and Date: 2010-05-03
• [Presentation] 選択的Rhoキナーゼ阻害剤による角膜内皮細胞遊走への影響 (2010)
  • Author(s): 奥村直毅, 小泉範子, 上野盛夫, 坂本雄二, 高橋浩昭, 平田香菜, 羽室淳爾, 木下茂
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-16
• [Presentation] 緑内障患者の近見視力障害と読字能力の相関 (2010)
  • Author(s): 加藤浩晃, 上野盛夫, 山村麻里子, 池田陽子, 今井浩二郎, 横山貴子, 森和彦, 木下茂
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-16
• [Presentation] 狭義開放隅角緑内障患者の血液生化学データの検討 (2010)
  • Author(s): 山脇敬博, 池田陽子, 今井浩二郎, 上野盛夫, 森和彦, 木下茂
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-16
• [Presentation] 多発性硬化症の病型別網膜神経線維厚減少量の検討 (2010)
  • Author(s): 池田陽子, 高橋純子, 森和彦, 上野盛夫, 永田真帆, 斎田孝彦, 木下茂
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-15
• [Presentation] 原発開放隅角緑内障疾患マーカーSNPsと全身疾患の関連性の検討 (2010)
  • Author(s): 池田陽子, 森和彦, 上野盛夫, 今井浩二郎, 八木知人, 大見奈津江, 徳田雄市, 不破正博, 田中雅深, 田代啓, 木下茂
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-15
• [Presentation] LOXL1遺伝子プロモーター領域の遺伝子多型と落屑緑内障の関係 (2010)
  • Author(s): 今井浩二郎, 森和彦, 上野盛夫, 池田陽子, 川崎諭, 中野正和, 谷口孝純, 大見奈津江, 田代啓, 木下茂
  • Organizer: 第114回日本眼科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-15
• [Book] 眼のサイエンス眼疾患の謎 (2010)
  • Author(s): 上野盛夫、池田陽子
  • Total Pages: 278
  • Publisher: 分光堂
• [Book] 専門医のための眼科臨床クオリファイ緑内障診療ガイド (2010)
  • Author(s): 上野盛夫、池田陽子
  • Total Pages: 273
  • Publisher: 中山書店
• [Book] 眼のサイエンス眼疾患の謎 (2010)
  • Author(s): 上野盛夫, 池田陽子
  • Total Pages: 278
  • Publisher: 文光堂
• [Book] 専門医のための眼科診療クオリファイ緑内障診療ガイド (2010)
  • Author(s): 上野盛夫, 池田陽子
  • Total Pages: 273
  • Publisher: 中山書店