Molecular cellular biological analysis and new drug development using retinal pigment epithelial cells isolated from aged macular degeneration monkeys

• Project/Area Number: 22791703
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: 独立行政法人国立病院機構東京医療センター(臨床研究センター) (2011); 独立行政法人国立病院機構(東京医療センター臨床研究センター) (2010)
• Principal Investigator: CHI Zai long 独立行政法人国立病院機構東京医療センター(臨床研究センター), 分子細胞生物学研究部, 研究員 (30567459)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 細胞・組織 / 病理学 / マイクロアレイ

Research Abstract

Retinal pigment epithelial cells were isolated and cultured from aged cynomolgus monkeys with drusen. RPE cells from affected monkeys were compared with normal. Tight junction of RPE cell, phagocytosis, autophagy, and gene expression were analyzed. Significant change was observed in RPE cells from affected monkeys.

Research Products

• [Journal Article] Suppression of drusen formation by compstatin, a peptide inhibitor of complement C3 activation, on Cynomolgus monkey with early-onset macular degeneration. Currrent Topics on Complement and Eye Disease (2011)
  • Author(s): Chi Z-L, Yoshida T, Lambris JD, and Iwata T.
  • Journal Title: Advances in Experimental Medicine and Biology Volume: 703 Pages: 127-135
• [Journal Article] Processing of optineurin in neuronal cells (2011)
  • Author(s): Shen X, Ying H, Qiu Y, Park J-S, Shyam R, Chi Z-L, Iwata T, Yue BYJT
  • Journal Title: The Journal of Biological Chemistry Volume: 286 Pages: 3618-29
• [Journal Article] Processing of optineurin in neuronal cells (2011)
  • Author(s): Shen X, et al
  • Journal Title: J Biol Chem Volume: 286 Pages: 3618-36294
  • Peer Reviewed
• [Journal Article] Overexpression of optineurin E50K disrupts Rab8 interaction and leads to a progressive retinal degeneration in mice (2010)
  • Author(s): Chi Z-L, Akahori, A, Obazawa M, Minami M, Noda T, Nakaya N, Tomarev S, Kawase K, Yamamoto T, Noda S, Sasaoka M, Shimazaki A, Takada Y, and Iwata T.
  • Journal Title: Human Molecular Genetics Volume: 19 Pages: 2605-2615
• [Journal Article] Mutant WDR36 directly affects axon growth of retinal ganglion cells leading to progressive retinal degeneration in mice (2010)
  • Author(s): Chi Z-L, Yasumoto F, Sergeev Y, Minami M, Obazawa M, Kimura I, Takada Y, and Iwata T.
  • Journal Title: Human Molecular Genetics Volume: 19 Pages: 3806-3815
• [Presentation] Microarray analysis of RPE cells isolated from cynomolgus monkey with early-onset drusen formation (2011)
  • Author(s): Chi, et al
  • Organizer: ARVO
  • Place of Presentation: Fort Lauderdale, FL
• [Presentation] Suppression of drusen formation by Compstatin (POT-4), a peptide inhibitor of C3 activation, on cynomolgus monkey with early-onset macular degeneration (2010)
  • Author(s): Z-L Chi(池在龍)
  • Organizer: XIX Biennial Meeting of the International Society for Eye Research (ISER 2010)
  • Place of Presentation: Hilton Montreal Bonaventure(カナダ)
  • Year and Date: 2010-07-19
• [Presentation] Analysis of Colocalization of Rab8 and ERM Family in the Ocular Body (2010)
  • Author(s): I. Kimura, H. Okamoto, Z.-L. Chi, M. Akahori, M. T. Suzuki, T. Iwata
  • Organizer: Association for Research in Vision and Ophthalmology
• [Presentation] CHARACTERIZATION OF GLIA CELLS IN OPTN AND WDR36 TRANSGENIC MICE (2010)
  • Author(s): T. Iwata. Z.-L. Chi, M. Akahori, Y. Takada, N. Nakaya, S. Tomarev, Y. Sergeev
  • Organizer: 19th International Congress for Eye Research
• [Presentation] Overexpression of mutant OPTN and WDR36 leads to a progressive retinal degeneration in mice (2010)
  • Author(s): Chi ZL. Akahori M, Obazawa M, Minami M, Noda T, Nakaya N, Tomarev S, Kawase K, Yamamoto T, Noda S, Sasaoka M, Shimazaki A, Sergeev Y, Takada Y, Iwata T.
  • Organizer: 50th American Society for Cell Biology
• [Presentation] Overexpression of Mutated Optineurin and WDR36 Leads to Normal Tension Glaucoma in Mice (2009)
  • Author(s): Chi ZL, et al.
  • Organizer: Association for Research in Vision and Ophthalmology
• [Presentation] 緑内障遺伝子WDR36トランスジェニックマウスの解析 (2009)
  • Author(s): 池在龍, 他
  • Organizer: 第113回日本眼科学会総会
• [Presentation] Susceptibility genes and animal models of glaucoma (2009)
  • Author(s): Chi ZL, et al.
  • Organizer: Asia Association for Research in Vision and Ophthalmology