The role of anti-apoptotic Bcl-X_L in bone formation and maintenance of bone mass

• Project/Area Number: 22791771
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Nagasaki University
• Principal Investigator: MORIISHI Takeshi 長崎大学, 大学院・医歯薬学総合研究科, 技術職員 (20380983)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 骨芽細胞 / アポトーシス / Bcl-XL / 骨形成

Research Abstract

In the present study, we generated transgenic mice with osteoblasts that express anti-apoptotic BCL-X_L. In BCL-X_L transgenic mice, bone volume was increased at 10 weeks of age and recognized maintenance of the bone volume at aging mice. In the recent aging society, bone volume decrease by the osteoporosis becomes the big social problem, therefore Bcl-X_L may become the target for the osteoporosis treatment in the future.

Research Products

• [Journal Article] OBIF, an osteoblast induction factor, plays an essential role in bone formation in association with osteoblastogenesis (2012)
  • Author(s): Mizuhashi K, Kanamoto T, Ito M, Moriishi T, Muranishi Y, Omori Y, Terada K, Komori T, Furukawa T.
  • Journal Title: Dev Growth Differ
  • NAID: 10031136918
• [Journal Article] SP7 inhibits osteoblast differentiation at a late stage in mice (2012)
  • Author(s): Yoshida CA, Komori H, Maruyama Z, Miyazaki T, Kawasaki K, Furuichi T, Fukuyama R, Mori M, Yamana K, Nakamura K, Liu W, Toyosawa S, Moriishi T, Kawaguchi H, Takada K, Komori T.
  • Journal Title: PLoS One Volume: 7(3)
  • NAID: 120006985432
• [Journal Article] Pyruvate dehydrogenase kinase 4 induces bone loss at unloading by promoting osteoclastogenesis (2012)
  • Author(s): Wang Y, Liu W, Masuyama R, Fukuyama R, Ito M, Zhang Q, Komori H, Murakami T, Moriishi T, Miyazaki T, Kitazawa R, Yoshida CA, Kawai Y, Izumi S, Komori T.
  • Journal Title: Bone Volume: 50(1) Pages: 409-19
• [Journal Article] OBIF, an osteoblast induction factor, plays an essential role in bone formation in association with osteoblastogenesis (2012)
  • Author(s): Mizuhashi K
  • Journal Title: Development, growth and differentiation Volume: 54 Issue: 4 Pages: 474-480
  • DOI: 10.1111/j.1440-169x.2012.01333.x
  • Peer Reviewed
• [Journal Article] Overexpression of Bcl2 in osteoblasts inhibits osteoblast differentiation and induces osteocyte apoptosis (2011)
  • Author(s): Moriishi T, Maruyama Z, Fukuyama R, Ito M, Miyazaki T, Kitaura H, Ohnishi H, Furuichi T, Kawai Y, Masuyama R, Komori H, Takada K, Kawaguchi H, Komori T.
  • Journal Title: PLoS One Volume: 6(11)
  • NAID: 120006985216
• [Journal Article] Early onset of Runx2 expression caused craniosynostosis, ectopic bone formation, and limb defects (2011)
  • Author(s): Maeno T, Moriishi T, Yoshida CA, Komori H, Kanatani N, Izumi S, Takaoka K, Komori T.
  • Journal Title: Bone Volume: 49(4) Pages: 673-82
• [Journal Article] Comparative morphology of the osteocyte lacunocanalicular system in various vertebrates (2011)
  • Author(s): Cao L, Moriishi T, Miyazaki T, Iimura T, Hamagaki M, Nakane A, Tamamura Y, Komori T, Yamaguchi A.
  • Journal Title: J Bone Miner Metab Volume: 29(6) Pages: 662-70
  • NAID: 10030895041
• [Journal Article] Overexpression of Bcl2 in osteoblasts inhibits osteoblast differentiation and induces osteocyte apoptosis (2011)
  • Author(s): Moriishi T, et al
  • Journal Title: PLoS One Volume: 6(11)
  • NAID: 120006985216
  • Peer Reviewed
• [Journal Article] Ultrastructural analysis of osteoblasts, osteocytes and odontoblasts in Runx2 transgenic mice. (2010)
  • Author(s): Miyazaki, T, Moriishi, T, Izumi, S, Baba, T, Komori, T
  • Journal Title: J Electr Microsc Technol Med Biol. Volume: 23(2) Pages: 50-50
• [Presentation] 2はマウス椎間板の発達維持に必須である (2011)
  • Organizer: 日本分子生物学会年会プログラム・要旨集
• [Presentation] 骨芽細胞分化における抗アポトーシス分子Bcl-2の役割 (2010)
  • Organizer: 第28回日本骨代謝学会学術集会学術集会プログラム抄録集
• [Presentation] 骨芽細胞分化における抗アポトーシス分子Bc1-2の役割 (2010)
  • Author(s): 森石武史
  • Organizer: 第28回日本骨代謝学会学術集会
  • Place of Presentation: 東京都新宿区