| Project/Area Number |
22791778
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Morphological basic dentistry
|
|---|
| Research Institution | Nihon University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | デキサメサゾン / トリプトファン誘導体 / 骨芽細胞 / 未分化間葉系幹細胞 / 共培養 / Osterix / 石灰化 |
|---|
| Research Abstract |
In this study, we investigated the effect of newly synthesized tryptophan derivatives and dexamethasone (Dex), a synthetic glucocorticoid, on osteoblast differentiation. The tryptophan derivatives and Dex enhanced terminal osteoblast differentiation, as indicated by mineralization. However, no inductive effect was observed on commitment of mesenchymal stem cells (MSC) into osteoblast differentiation was observed. Furthermore, it was suggested that a transcription factor, Osterix, played an important role in the mechanism by which tryptophan derivatives and dexamethasone induced terminal osteoblast differentiation. In addition, we demonstrated that these agents significantly induced mRNA expression levels of the osteogenic marker genes bone sialoprotein (BSP) and Alkaline Phosphatase (ALP) in MSCs co-cultured with osteoblasts.
|
