Observation of ultra-structures in resin-bonding interface by Ultra-high Voltage Electron Tomography

• Project/Area Number: 22791831
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: MIURA Jiro 大阪大学, 歯学部附属病院, 助教 (70437383)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 石灰化硬組織 / 電子線トモグラフィー / ナノテクノロジー / 象牙質 / コラーゲン / 電子顕微鏡 / 硬組織 / 接着界面 / トモグラフィー

Research Abstract

Human dentin are hard and brittle mineralized tissues. It is difficult to prepare thin specimens (under 200 nm) of these tissues for transmission electron microscope observation without demineralizing them. We present a novel method for creating three-dimensional ultra-structural images of interface of resin and dentin bonding.

Research Products

• [Journal Article] Structural Modifications of Dentinal Microcracks with human aging,Microscopy (2013)
  • Author(s): Kubo M, Miura J, Sakata T, Nishi R, Takeshige F
  • Journal Title: 学術論文 Volume: (in press)
  • Peer Reviewed
• [Journal Article] Mechanical and morphological evaluation of the bond-dentin interface in direct resin core build-up method (2013)
  • Author(s): Matsumoto M, Miura J, Takeshige F, Yatani H
  • Journal Title: Dental Materials Volume: 29 Pages: 287-293
  • Peer Reviewed
• [Journal Article] Detection of dentinal microcracks using Infrared Thermography (2013)
  • Author(s): Matsushita-Tokugawa M, Miura J, Iwami Y, et al
  • Journal Title: Journal of Endodontics Volume: 39 Pages: 88-91
  • Peer Reviewed
• [Journal Article] 1.Structural Modifications of Dentinal Microcracks with human aging (2013)
  • Author(s): Kubo M, Miura J, Sakata T, Nishi R, Takeshige F
  • Journal Title: Microscopy Volume: in press
  • Peer Reviewed
• [Journal Article] Ultrastructural observation of human enamel and dentin by ultra-high voltage electron tomography and focus ion beam technique (2012)
  • Author(s): Miura J, Kubo M, Nagashima T, Takeshige F
  • Journal Title: Journal of Electron Microscopy Volume: 61(5) :335-41
  • Peer Reviewed
• [Journal Article] 歯の形成と老化の可視化 (2012)
  • Author(s): 福島修一郎、荒木勉、三浦治郎
  • Journal Title: O plus E Volume: 34 Pages: 1051-1055
• [Journal Article] レジン支台築造法における象牙質接着界面の多面的評価 (2012)
  • Author(s): 三浦治郎
  • Journal Title: Multi-Function Nanofoundary ISIR & UHVEM Volume: 1 Pages: 148-51
• [Journal Article] Biomedical applications of a real-time terahertz color scanner (2010)
  • Author(s): M Schirmer, M Fujio, M Minami, J Miura, T Araki, T Yasui
  • Journal Title: Optics Express Volume: 1 Pages: 354-366
  • Peer Reviewed
• [Journal Article] 診る技術を支える見る技術 (2010)
  • Author(s): 三浦治郎
  • Journal Title: 生産と技術 Volume: 春号 Pages: 78-81
• [Presentation] 歯科医療における界面構造観察の有用性 (2013)
  • Author(s): 三浦治郎
  • Organizer: 微細構造解析プラットフォーム第2回ワークショップ(招待講演)NIMS筑波
  • Place of Presentation: つくば市
  • Year and Date: 2013-03-01
• [Presentation] Fluorescence Characteristics and Morphological evaluation of Advanced Glycation End Products in Human Dentin (2013)
  • Author(s): Jiro Miura, Mizuho Kubo, Kantaro Nishikawa, Shuichiro Fukushima, Fumio Takeshige, and Tsutomu Araki
  • Organizer: IADR
  • Place of Presentation: Seattle, United States
• [Presentation] Structural Modifications of dentinal microcracks with human aging (2013)
  • Author(s): Mizuho Kubo, Jiro Miura, Masato Shimizu, Tadashi Nagashima, Fumio Takeshige
  • Organizer: IADR
  • Place of Presentation: Seattle, United States
• [Presentation] 歯科医療における界面構造観察の有用性 (2013)
  • Author(s): 三浦治郎
  • Organizer: 微細構造解析プラットフォーム第２回ワークショップ（招待講演）
  • Place of Presentation: 物質材料研究機構（筑波市）
  • Invited
• [Presentation] Fluorescence Characteristics and Morphological evaluation of Advanced Glycation End Products in Human Dentin (2013)
  • Author(s): Jiro Miura, Mizuho Kubo, Kantaro Nishikawa, Shuichiro Fukushima, Fumio Takeshige, and Tsutomu Araki
  • Organizer: International Association for Dental Research
  • Place of Presentation: Seattle, United States
• [Presentation] Structural Modifications of dentinal microcracks with human aging (2013)
  • Author(s): Mizuho Kubo, Jiro Miura, Masato Shimizu, Tadashi Nagashima, Fumio Takeshige
  • Organizer: International Association for Dental Research
  • Place of Presentation: Seattle, United States
• [Presentation] 象牙質接着界面評価における電子線トモグラフィー用切片加工法の検討 (2012)
  • Author(s): 三浦治郎、久保美寿穂、清水真人、長島正、竹重文雄
  • Organizer: 第137回日本歯科保存学会
  • Place of Presentation: 広島市
  • Year and Date: 2012-11-21
• [Presentation] 加齢による象牙質の形態変化と微小亀裂伸展様相の検討 (2012)
  • Author(s): 久保美寿穂、三浦治郎、木下可子、西藤三紀子、長島正、竹重文雄
  • Organizer: 第137回日本歯科保存学会
  • Place of Presentation: 広島市
  • Year and Date: 2012-11-21
• [Presentation] 糖化によるヒト象牙質の蛍光と力学特性の変化 (2012)
  • Author(s): 西川貫太郎 近藤恵太 田中佑治 三浦治郎 荒木勉
  • Organizer: 日本バイオフロンティア学会
  • Place of Presentation: 青森
  • Year and Date: 2012-10-05
• [Presentation] Influence of Glycosylation on Fluorescence Characteristics and Mechanical Properties of Human Dentin (2012)
  • Author(s): Miura J
  • Organizer: 1stInternatinal Conference on Fluorescense-based diagnostic of Oral Diseases
  • Place of Presentation: Montpellier France
  • Year and Date: 2012-09-20
• [Presentation] Evaluation of Adhesion of Resin to Radicular Dentin in a Direct Resin Core Build-up Method (2012)
  • Author(s): Matsumoto M、Miura J, Mine A, Yatani H
  • Organizer: 12th Asian Academy of Aesthetic Dentistry
  • Place of Presentation: Sapporo Japan
  • Year and Date: 2012-07-20
• [Presentation] 未脱灰石灰化硬組織における電子線トモグラフィー用切片加工法 (2012)
  • Author(s): 三浦治郎、長谷川利昭、久保美寿穂、長島正、竹重文雄
  • Organizer: 第68回日本顕微鏡学会学術講演会
  • Place of Presentation: 筑波市
  • Year and Date: 2012-05-14
• [Presentation] Influence of Glycosylation on Fluorescence Characteristics and Mechanical Properties of Human Dentin (2012)
  • Author(s): Jiro Miura
  • Organizer: 1st Internatinal Conference on Fluorescense-based diagnostic of Oral Diseases
  • Place of Presentation: Montpellier France
• [Presentation] Evaluation of Adhesion of Resin to Radicular Dentin in a Direct Resin Core Build-up Method (2011)
  • Author(s): Mariko Matsumoto, Jiro Miura, Hirofumi Yatani
  • Organizer: 国際接着歯科学会
  • Place of Presentation: Seoul
  • Year and Date: 2011-04-21
• [Presentation] 直接法レジンコア築造法における根面接着様相の評価 (2011)
  • Author(s): 松本真理子, 三浦治郎, 矢谷博文
  • Organizer: 日本接着歯科学会
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-02-05
• [Presentation] 新規デュアルキュア型レジンコア材料を用いた支台築造システムにおける根面接着様相の評価 (2011)
  • Author(s): 松本真理子、三浦治郎,矢谷博文
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 大阪
• [Presentation] 赤外線サーモグラフィを用いた歯根破折診断法の開発 (2011)
  • Author(s): 松下真美,三浦治郎,竹重文雄,岩見行晃,阪上隆英,和泉遊以,林美加子,今里聡,恵比須繁之
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 大阪
• [Presentation] Evaluation of Adhesion of Resin to Radicular Dentin in a Direct Resin Core Build-up Method (2011)
  • Author(s): Mariko Matsumoto, Jiro Miura, Hirofumi Yatani
  • Organizer: International Congress on Adhesive Dentistry
  • Place of Presentation: Seoul
• [Presentation] Three-dimensional ultrastructureof the interfaces between dentin and composite resin (2010)
  • Author(s): Jiro Miura , Noriaki Hasegawa, Tadashi Nagashima, Fumio Takeshige
  • Organizer: International Association for Dental Research
  • Place of Presentation: Barcelona Spain