| Project/Area Number |
22791940
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
IWATA Takanori 東京女子医科大学, 医学部, 講師 (60431946)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 歯根膜細胞 / 多分化能 / 細胞治療 / 細胞標識 / 神経堤細胞 / ヒト歯根膜細胞 / NCAM1 / 神経細胞分化 |
|---|
| Research Abstract |
Neural differentiation potential of human periodontal ligament(PDL) cells was examined. PDL tissue-derived cells showed mesenchymal stromal cells(MSC)-like properties and possessed neural differentiation capacities both in vitro and in vivo. In addition, we found out that c-kit, a key marker of hematopoiesis, was slightly(～ 0.5%) expressed in human PDL cells. Thus, we are studying the involvement of c-kit/stem cell factor signaling during cell differentiation. To trace the transplanted cells, we introduced lentiviral system and optimize the methods of decalcification and fixation to detect the GFP-labeled cells in immunohistochemical analysis.
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