Quantitative proteomics using formalin-fixed paraffin-embedded tissues of oral cancer

• Project/Area Number: 22791959
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: NEGISHI Ayako 東京医科歯科大学, 歯学部附属病院, 医員 (70549972)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: プロテオーム / 口腔がん / バイオマーカー / 質量分析 / ホルマリン固定パラフィン包埋組織 / 放射線化学療法 / 抗がん剤 / プロテオーム解析

Research Abstract

Neoadjuvant chemotherapy is used to treat oral cancer however chemo-resistance is a major obstacle. The ability to predict tumour response would allow chemotherapy administration to be directed towards patients who would most benefit, thus maximising treatment efficacy. This study aimed to identify protein biomarkers associated with response to neoadjuvant chemotherapy using formalin-fixed paraffin-embedded(FFPE) tissue blocks of oral squamous cell carcinoma samples and analyzed by 2-dimensional image-converted analysis of liquid chromatography and mass spectrometry(2DICAL), a label-free quantitative proteomics method.