Bone tissue engineering by human dental pulp cells

• Project/Area Number: 22791960
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: HAMADA Keiichi 東京医科歯科大学, 歯学部, 非常勤講師 (60570025)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 骨 / 再生医療 / 歯髄 / 無血清培地 / 歯

Research Abstract

The aim of this study is to develop a new way of bone tissue engineering by human dental pulp cells cultured without serum and osteogenic differentiation induction. Human dental pulp cells could be cultured and expanded in serum-free medium. There were no differences in alkaline phosphatase activity and expression of osteocalin between human dental pulp cell expanded in serum-free medium and in medium containing fetal bovine serum. Human dental pulp cells expanded in serum free medium formed mineralized tissues in vivo, but it is not typical bone tissues.

Research Products

• [Journal Article] 放射線による歯根形成阻害メカニズムの検討 (2011)
  • Author(s): 阿部成宏、濱田啓一、山口聰、天笠光雄
  • Journal Title: 日本口腔組織培養学会雑誌 Volume: 20(1) Pages: 41-41
• [Journal Article] Characterization of the radioresponse of human apical papilla-derived cells. (2011)
  • Author(s): Abe S., Hamada K., Yamaguchi S., et al
  • Journal Title: Stem Cell Res.Therap. Volume: 2:2
  • Peer Reviewed
• [Journal Article] Characterization of the radioresponse of human apical-papilla derived cells (2010)
  • Author(s): Abe s., Hamada k., Yamaguchi S., Amagasa T., Miura M.
  • Journal Title: Stem Cell Res. Therap Volume: 2 Issue: 1 Pages: 2-2
  • DOI: 10.1186/scrt43
  • Peer Reviewed
• [Journal Article] ヒト根未完成歯根尖部歯髄組織由来細胞は神経堤幹細胞様の性質を持つ (2010)
  • Author(s): 山口聰、阿部成宏、濱田啓一、天笠光雄
  • Journal Title: 日本口腔組織培養学会雑誌 Volume: 19(1) Pages: 37-38
• [Presentation] ヒト根未完成歯・根尖部歯髄由来細胞の放射線生物学的検討 (2011)
  • Author(s): 阿部成宏、濱田啓一、山口聰、天笠光雄、山城正司
  • Organizer: 第56回日本口腔外科学会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-21
• [Presentation] ヒト根未完成歯根尖部歯髄由来細胞の放射線生物学的検討 (2011)
  • Author(s): 阿部成宏、濱田啓一, 他
  • Organizer: 第56回日本口腔外科学会総会・学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-21
• [Presentation] 放射線による歯根形成阻害メカニズム (2010)
  • Author(s): 阿部成宏、山口聰、濱田啓一、天笠光雄
  • Organizer: 第46回日本口腔組織培養学会
  • Place of Presentation: 高知
  • Year and Date: 2010-11-13
• [Presentation] 放射線による歯根形成阻害メカニズムの検討 (2010)
  • Author(s): 阿部成宏、濱田啓一、山口聰, 他
  • Organizer: 第47回日本口腔組織培養学会
  • Place of Presentation: 高知市
  • Year and Date: 2010-11-13
• [Presentation] 分化誘導因子を必要としないヒト歯髄組織由来細胞による骨組織形成 (2010)
  • Author(s): 山口聰、阿部成宏、濱田啓一、天笠光雄
  • Organizer: 第8回日本歯科再生医療学会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-10-30