| Project/Area Number |
22792053
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
SHIRAKURA Maya 広島大学, 大学院・医歯薬学総合研究科, 特任助教 (70549013)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 歯科矯正学 / 変形性顎関節症 / HIF-1α / 低酸素 / 破骨細胞 / 機械刺激 / 炎症性サイトカイン |
|---|
| Research Abstract |
Giving mechanical stress, the cells were cultured under 1%, 5%, 10%, 15% pO2, and normoxic condition and HIF-1 activity was analyzed. As a result, HIF-1 was observed only under 1% pO2 condition without loading. On the other hand, HIF-1 tended to increase under every condition with loading. Furthermore the activation of HIF-1 was evaluated by a real-time RT-PCR analysis for HIF-1 target genes and osteoclast-relating genes. As a result, mechanical stimuli strikingly increased these genes expression under 1% and 5% pO2.
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