the molecular mechanism of dorso-ventral patterning in late stages of development

• Project/Area Number: 22870008
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: TSUKAHARA Tatsuya 東京大学, 大学院・理学系研究科, 助教 (90586413)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000); Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000); Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
• Keywords: 後期発生 / 背腹パターン / Zic1/Zic4 / ChIP-seq

Research Abstract

We analyzed the molecular function of transcription factors Zic1/Zic4, which express in dorsal somites and regulate dorso-ventral patterning of body trunk. We performed transcriptome analyses using Da mutants, which lost the expression of Zic1/Zic4 specifically in somites and identified several downstream genes of Zic1/Zic4. We found that the modulator of Notch signaling, lunatic fringe, is one of the downstream target of Zic1/Zic4, suggesting that Zic1/Zic4 contribute the dorsal trunk morphology by reguating the activity of Notch signaling. We generated and analyzed several mouse lines to understand the conserved function and expression of Zic1/Zic4.

Research Products

• [Journal Article] The Medaka zic1/zic4 Mutant Provides Molecular Insights into Teleost Caudal Fin Evolution (2012)
  • Author(s): Moriyama Y, Kawanishi T, Nakamura R, Tsukahara T, Sumiyama K, Suster ML, Kawakami K, Toyoda A, Fujiyama A, Yasuoka Y, Nagao Y, Sawatari E, Shimizu A, Wakamatsu Y, Hibi M, Taira M, Okabe M, Naruse K, HashimotoH, Shimada A, Takeda H
  • Journal Title: Current biology Volume: vol.22 Pages: 601-607
  • URL: http://dx.doi.org/10.1016/j.cub.2012.01.063
• [Journal Article] The medaka zic1/zic4 mutant provides molecular insights into teleost caudal fin evolution. (2012)
  • Author(s): Yuuta Moriyama
  • Journal Title: Current Biology Volume: 21 Issue: 7 Pages: 601-607
  • DOI: 10.1016/j.cub.2012.01.063
  • Peer Reviewed
• [Presentation] The identification of the downstream genes of Zic1/Zic4, key reg ulators of dorso-ventral patterning (2011)
  • Author(s): Yoshikawa K, Nishimura T, Tanaka M, Shigenobu S, Saito TL, Yoshimura J, Morishita S, Tsukahara T, Takeda H
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] The identification of the downstream genes of Zic1/Zic4, key regulators of dorso-ventral patterning (2011)
  • Author(s): Yoshikawa K, Nishimura T, Tanaka M, Shigenobu S, Saito TL, Yoshimura J, MorishitaS, Tsukahara T and Takeda H
  • Organizer: 第34回日本分子生物学会年会
• [Presentation] Zicl/Zic4による背腹パターン形成機構の普遍性と分子メカニズム (2010)
  • Author(s): 塚原達也
  • Organizer: 新学術領域(秩序形成ロジック)第一回班会議
  • Place of Presentation: 山形県 蔵王温泉
  • Year and Date: 2010-10-11
• [Presentation] Zic1/Zic4による背腹パターン形成機構の普遍性と分子メカニズム (2010)
  • Author(s): 塚原達也
  • Organizer: 新学術領域(秩序形成ロジック)第一回班会議