Structural and biochemical analyses of interactions among TSWV structural proteins

Research Project

Project/Area Number	22880001
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Plant pathology
Research Institution	Hokkaido University
Principal Investigator	KOMODA Keisuke 北海道大学, 大学院・先端生命科学研究院, 特任助教 (40581640)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000) Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000) Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
Keywords	ウィルス / 蛋白質 / X線結晶構造解析 / TSWV / N蛋白質 / Nタンパク質
Research Abstract	The nucleocapsid proteins(NP) of Tomato Spotted Wilt Virus(TSWV) are key proteins in the replication of viral RNA. In this study, we obtained crystals of recombinant TSWV NPs diffracting to 2. 7Åresolution. The asymmetric unit contains three NP molecules forming trimeric ring-like structure. Moreover, the inside of TSWV NP trimeric ring shows the positive charge rich surface, suggesting that these regions are expected to be genomic RNA binding site.

Report

(3 results)