Research on the synergistic enhancement of physiological functions by food components

• Project/Area Number: 22880024
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Food science
• Research Institution: Kyushu University
• Principal Investigator: TANAKA Mitsuru 九州大学, 農学研究院, 特任助教 (70584209)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥2,795,000 (Direct Cost: ¥2,150,000、Indirect Cost: ¥645,000); Fiscal Year 2011: ¥1,209,000 (Direct Cost: ¥930,000、Indirect Cost: ¥279,000); Fiscal Year 2010: ¥1,586,000 (Direct Cost: ¥1,220,000、Indirect Cost: ¥366,000)
• Keywords: 食相乗性 / ペプチド / 血管機能 / エピガロカテキンガレート

Research Abstract

The aim of this study is to demonstrate the enhancement of the small peptide Trp-His-induced vasorelaxation by food components. We found that epigallocatechin gallate(EGCg) dramatically enhanced the Trp-His-induced vasorelaxation. The present study also suggests that the Trp-His-induced vasorelaxation is significantly potentiated by the activation of the NO/cGMP pathway by EGCg.

Research Products

• [Journal Article] The anti-therosclerotic di用eptide, Trp-His, inhibits the phosphorylation of voltage-dependent L-type Ca^2+ channels in rat vascular smooth muscle cells (2012)
  • Author(s): Y. Kobayashi, T. Fukuda, M. Tanaka, T. Matsui
  • Journal Title: FEBS Open Bio Volume: 2 Pages: 83-88
  • Peer Reviewed
• [Journal Article] Effect of the Uncharged Imidazolium Moiety in Adenine on Endothelium-Independent Relaxation in Contracted Thoracic Aorta of Sprague-Dawley Rats (2012)
  • Author(s): S.Tokuda, et al
  • Journal Title: Biosci.Biotechnol.Biocbem. Volume: (in accept)
  • Peer Reviewed
• [Journal Article] 血管機能とペプチド (2011)
  • Author(s): 松井利郎, 田中充
  • Journal Title: New Food Industry Volume: 53 Pages: 22-30
  • NAID: 40018729449
• [Journal Article] Application of ^<13>C-stable-isotope labeling LC-MRM-MS/MS method for determining intact absorption of bioactive di-peptides into blood system in spontaneously hypertensive rats. (2011)
  • Author(s): E.M.N.Nakashima, et al.
  • Journal Title: Anal.Biochem. Volume: (in accept)
  • Peer Reviewed
• [Journal Article] Tip-His, a vasorelaxant di用eptide, can inhibit extra cellular Ca' entry to rat vascular smooth muscle cells through blockade of dihydropyridine-like L-type Ca^<2+> channels (2010)
  • Author(s): Z. Wang, S. Watanabe, Y. Kobayashi, M. Tanaka, T. Matsui
  • Journal Title: Peptides Volume: 31 Pages: 2060-2066
  • Peer Reviewed
• [Journal Article] Trp-His, a vasorelaxant di-peptide, can inhibit extracellular Ca2+ entry to rat vascular smooth muscle cells through blockade of dihydropyridine-like L-type Ca^<2+> channels. (2010)
  • Author(s): Z.Wang, et al.
  • Journal Title: Peptides Volume: 31 Pages: 2060-2066
  • Peer Reviewed
• [Presentation] 低分子ペプチドTrp-HisとEGCgとの相乗的血管弛緩作用はNO/cGMP系賦活化を介て発現する (2012)
  • Author(s): 田中充、須山晶,趙健,松井利郎
  • Organizer: 第66回日本栄養・食糧学会大会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2012-05-19
• [Presentation] 抗動脈硬化ペプチドTrp-Hisは血管平滑筋細胞でのL型Ca2+チャンネルのリン酸化を抑制する (2012)
  • Author(s): 福田俊彦,小林優多郎,田中充松井利郎
  • Organizer: 第66回日本栄養・食糧学会大会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2012-05-19
• [Presentation] グリシニン酵素加水分解物からの血管平滑筋細胞内Ca2+流入阻害ペプチドの検索 (2011)
  • Author(s): 松村慎也,王正全,田中充,松井利郎
  • Organizer: 平成23年日本栄養・食糧学会九州・沖縄支部及び日本食品科学工学会西日本支部合同大会
  • Place of Presentation: 佐賀大学(佐賀県)
  • Year and Date: 2011-09-04
• [Presentation] 血管平滑筋細胞における抗動脈硬化ペプチドTrp-Hisの細胞内Ca^<2+>シグナル系制御機構の解明 (2011)
  • Author(s): 小林優多郎,福田俊彦,田中充,松井利郎
  • Organizer: 平成23年日本栄養・食糧学会九州・沖縄支部及び日本食品科学工学会西日本支部合同大会
  • Place of Presentation: 佐賀大学(佐賀県)
  • Year and Date: 2011-09-04
• [Presentation] グリシニン酵素加水分解物からの血管平滑筋細胞内Ca^<2+>流入阻害ペプチドの検索 (2011)
  • Author(s): 松村慎也, ら
  • Organizer: 平成23年日本栄養・食糧学会九州・沖縄支部及び日本食品科学工学会西日本支部合同大会
  • Place of Presentation: 佐賀大学(佐賀県)
  • Year and Date: 2011-09-04
• [Presentation] 低分子ペプチドの生理機能相乗作用に関する研究(1);ポリフェノールによる血管弛緩性ペプチドの増強作用 (2011)
  • Author(s): 田中充,松井利郎
  • Organizer: 第65回日本栄養・食糧学会大会
  • Place of Presentation: お茶の水女子大学(東京都)
  • Year and Date: 2011-05-14
• [Presentation] 低分子ペプチドの生理機能相乗作用に関する研究(1);ポリフェノールによる血管弛緩性ペプチドの増強作用 (2011)
  • Author(s): 田中充, 松井利郎
  • Organizer: 第65回日本栄養・食糧学会大会
  • Place of Presentation: 東京農業大学(東京都)
  • Year and Date: 2011-05-14
• [Presentation] Matsui. Antiatherosclerotic effect of a small peptide, Tip-His, in apolipoprotein E deficient mice (2010)
  • Author(s): M. Tanaka, Z. Wang, Y. Kobayashi, T.
  • Organizer: China-Japan Joint Symposium on Food Science & Technology of Food Industry Level
  • Place of Presentation: 中国
  • Year and Date: 2010-11-03
• [Presentation] A small peptide, Tip-His, that improves vascular health can act as a Ca^<2+> channel blacker (2010)
  • Author(s): Y. Kobayashi, Z. Wang, M. Tanaka, T. Matsui
  • Organizer: China-Japan Joint Symposium on Food Science & Technology of Food Industry Level
  • Place of Presentation: 中国
  • Year and Date: 2010-11-03
• [Presentation] Anti-atherosclerotic effect of a small peptide, Trp-His, in apolipoprotein E deficient mice. (2010)
  • Author(s): M.Tanaka, et al.
  • Organizer: China-Japan Joint Symposium on Food Science & Technology of Food Industry Level
  • Place of Presentation: 中国
  • Year and Date: 2010-11-03
• [Presentation] A small peptide, Trp-His, that improves vascular health can act as a Ca2+ channel blocker. (2010)
  • Author(s): Y.Kobayashi, et al.
  • Organizer: China-Japan Joint Symposium on Food Science & Technology of Food Industry Level
  • Place of Presentation: 中国
  • Year and Date: 2010-11-03
• [Presentation] Antiatherosclerotic dipeptide, Tip-His, can suppress intracellular[ Ca^<2+>] in vascular smooth muscle cells in a similar manner to dihydropyridine type Ca^<2+> channel blocker (2010)
  • Author(s): Z. Q. Wang, M. Tanaka, S. Watanabe, Y. Kobayashi, T. Matsui
  • Organizer: The 23rd Scientific Meeting of the International Society of Hypertension
  • Place of Presentation: Canada
  • Year and Date: 2010-09-30
• [Presentation] Anti-atherosclerotic di-peptide, Trp-His, can suppress intracellular [Ca^<2+>] in vascular smooth muscle cells in a similar manner to dihydropyridine type Ca^<2+> channel blocker. (2010)
  • Author(s): Z.Q.Wang, et al.
  • Organizer: The 23rd Scientific Meeting of the International Society of Hypertension
  • Place of Presentation: Canada
  • Year and Date: 2010-09-30
• [Presentation] 血管平滑筋細胞を用いた血管機能改善ジペプチドTrp-HisのL型Ca^<2+>チャンネルに対する結合部位の解明 (2010)
  • Author(s): 小林優多郎,王正全,渡邊慎平,田中充,松井利郎
  • Organizer: 第57回日本食品科学工学会
  • Place of Presentation: 東京農業大学(東京都)
  • Year and Date: 2010-09-03
• [Book] Chapter 2. Vascular regulation by small peptides : Bioactive Natural Products : Opportunities & Ch all enges in Medicinal Chemistry (2012)
  • Author(s): T. Matsui, Z. Wang, M. Tanaka
  • Publisher: World Scientific Publishing Co.
• [Book] Chapter 2. Vascular regulation by small peptides in "Bioactive Natural Products : Opportunities & Challenges in Medicinal Chemistry" (2012)
  • Author(s): T.Matsui, et al
  • Total Pages: 21
  • Publisher: World Scientific Publishing Co
• [Book] Chapter 3. Vascular regulation by small peptides in "Bioactive Natural Products : Opportunities & Challenges in Medicinal Chemistry" (2011)
  • Author(s): T.Matsui, et al.
  • Publisher: World Scientific Publishing Co.(in press)
• [Book] Chapter 4. Antihypertensive peptides and their underlying mechanism : Bioactive Proteins and Peptides as Functional Foods and Nu traceu ticals (2010)
  • Author(s): T. Matsui, M. Tanaka
  • Publisher: Institute of Food Technologists
• [Book] Chapter 4. Anti-hypertensive peptides and their underlying mechanism in "Bioactive Proteins and Peptides as Functional Foods and Nutraceuticals" (2010)
  • Author(s): T.Matsui, et al.
  • Publisher: IFT (Blackwell Publishing Ltd.)