Biological significance of the unique fatty acid composition of phosphatidylinositol in the asymmetric division of stem cells.

• Project/Area Number: 22890032
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Tokyo Women's Medical University (2011); The University of Tokyo (2010)
• Principal Investigator: IMAE Rieko 東京女子医科大学, 医学部, 助教 (60584000)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,146,000 (Direct Cost: ¥2,420,000、Indirect Cost: ¥726,000); Fiscal Year 2011: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000); Fiscal Year 2010: ¥1,638,000 (Direct Cost: ¥1,260,000、Indirect Cost: ¥378,000)
• Keywords: 非対称分裂 / ホスホイノシチド

Research Abstract

Intracellular phospholipase A1(ipla-1) and acyltransferases(acl-8, acl-9 and acl-10) determine the sn-1 fatty acyl chain of phosphatidylinositol(PI) in C. elegans. In both ipla-1 mutants and acl-8 acl-9 acl-10 triple mutants, the sn-1 fatty acid composition of PI is significantly altered, and asymmetric division of stem cell-like epithelial cells is defective. This study identified the molecules that are associated with the asymmetric division defects caused by the altered fatty acid composition of PI. In addition, functional conservation of acl-8,-9,-10 in mammals was examined.

Research Products

• [Journal Article] LYCAT, a homologue of C. elegans acl-8, acl-9, and acl-10, determines the fatty acid composition of phosphatidylinositol in mice (2012)
  • Author(s): Rieko Imae, Takao Inoue, Yasuko Nakasaki, Yasunori Uchida, Yohsuke Ohba, Nozomu Kono, Hiroki Nakanishi, Takehiko Sasaki, Shohei Mitani and Hiroyuki Arai
  • Journal Title: Journal of Lipid Research Volume: 53 Issue: 3 Pages: 335-347
  • DOI: 10.1194/jlr.m018655
  • Peer Reviewed
• [Journal Article] Intracellular PLA1 and acyltransferase, which are involved in C. elegans stem cell divisions, determine the sn-1 fatty acyl chain of phosphatidylinositol (2010)
  • Author(s): Imae, R., Inoue, T., et al(8名中2番目)
  • Journal Title: Mol. Biol. Cell Volume: 21 Issue: 18 Pages: 3114-3124
  • DOI: 10.1091/mbc.e10-03-0195
  • Peer Reviewed
• [Journal Article] Intracellular Phospholipase A_1 and Acyltransferase, Which Are Involved in Caenorhabditis elegans Stem Cell Divisions, Determine the sn-1 Fatty Acyl Chain of Phosphatidylinositol (2010)
  • Author(s): Rieko Imae, Takao Inoue, Masako Kimura, Takahiro Kanamori, Naoko H. Tomioka, Eriko Kage-Nakadai, Shohei Mitani, Hiroyuki Arai
  • Journal Title: Molecular Biology of the Cell Volume: VOL.21 Pages: 3114-3124
  • Peer Reviewed
• [Presentation] Intracellular PLA1 and acyltransferase, which are involved in C. elegans stem cell divisions, determine the sn-1 fatty acyl chain of phosphatidylinositol (2011)
  • Author(s): Rieko Imae, Takao Inoue, Masako Kimura, Eriko Kage-Nakadai, Shohei Mitani, Hiroyuki Arai
  • Organizer: 18th International C. elegans Meeting
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2011-06-23
• [Presentation] Intracellular PLA1 and acyltransferase, which are involved in C.elegans stem cell divisions, determine the sn-1 fatty acyl chain of phosphatidy linositol (2011)
  • Author(s): Rieko Imae, Takao Inoue, Masako Kimura, Eriko Kage-Nakadai, Shohei Mitani, Hiroyuki Arai
  • Organizer: 18^<th> International C.elegans Meeting
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2011-06-23
• [Presentation] Intracellular PLA1 and acyltransferase, which are involved in C. elegans stem cell divisions, determine the sn-1 fatty acyl chain of phosphatidylinositol (2010)
  • Author(s): Rieko Imae, Takao Inoue, Masako Kimura, Eriko Kage-Nakadai, Shohei Mitani, Hiroyuki Arai
  • Organizer: ASCB(the American Society for Cell Biology) 50th Annual Meeting
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2010-12-13
• [Presentation] Intracellular Phospholipase A_1 and Acyltransferase, Which Are Involved in Caenorhabditis elegans Stem Cell Divisions, Determine the sn-1 Fatty Acyl Chain of Phosphatidylinositol (2010)
  • Author(s): Rieko Imae, Takao Inoue, Masako Kimura, Eriko Kage-Nakadai, Shohei Mitani, Hiroyuki Arai
  • Organizer: ASCB (the American Society for Cell Biology) 50th Annual Meeting
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2010-12-13
• [Presentation] Intracellular PLA1 and acyltransferase, which are involved in C. elegans stem cell divisions, determine the sn-1 fatty acyl chain of phosphatidylinositol (2010)
  • Author(s): Rieko Imae, Takao Inoue, Masako Kimura, Eriko Kage-Nakadai, Shohei Mitani, Hiroyuki Arai
  • Organizer: 4th East Asia C. elegans Meeting
  • Place of Presentation: Tokyo
  • Year and Date: 2010-07-13
• [Presentation] Intracellular Phospholipase A_1 and Acyltransferase, Which Are Involved in Caenorhabditis elegans Stem Cell Divisions, Determine the sn-1 Fatty Acyl Chain of Phosphatidylinositol (2010)
  • Author(s): Rieko Imae, Takao Inoue, Masako Kimura, Eriko Kage-Nakadai, Shohei Mitani, Hiroyuki Arai
  • Organizer: 4^<th> East Asia C. elegans Meeting
  • Place of Presentation: 国立オリンピック記念青少年総合センター(東京都)
  • Year and Date: 2010-07-13
• [Remarks] NBRPホームページアドレス
  • URL: http://www.shigen.nig.ac.jp/c.elegans/index.jsp