Functional roles of cis-antisense gene of MYCN, NCYM, in human neuroblastoma

• Project/Area Number: 22890241
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Pediatrics
• Research Institution: Chiba Cancer Center (Research Institute)
• Principal Investigator: SUENAGA Yusuke 千葉県がんセンター(研究所), がん治療開発グループ, 研究員 (80581793)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,068,000 (Direct Cost: ¥2,360,000、Indirect Cost: ¥708,000); Fiscal Year 2011: ¥1,469,000 (Direct Cost: ¥1,130,000、Indirect Cost: ¥339,000); Fiscal Year 2010: ¥1,599,000 (Direct Cost: ¥1,230,000、Indirect Cost: ¥369,000)
• Keywords: NCYM / MYCN / 神経芽腫 / Natural Antisense RNA / 遺伝子増幅 / GSK3β / p53ファミリー遺伝子 / GSK3 / p63 / P53ファミリー遺伝子 / P63 / 転写制御

Research Abstract

MYCN oncogene is frequently amplified in neuroblastoma, and MYCN contributes to aggressiveness of the tumors. NCYM is a cis-antisense transcript of MYCN gene and is co-amplified with MYCN gene in unfavorable neuroblastoma. However, the functional roles of NCYM in neuroblastoma remain elusive. In this study, we found that NCYM gene encodes nuclear protein, and the NCYM protein stabilizes MYCN to maintain the cellular survival of neurblastomas.

Research Products

• [Journal Article] RUNX3 modulates DNA damage-mediated phosphorylation of tumor suppressor p53 at Ser-15 and acts as a Co-activator for p53 (2010)
  • Author(s): Yamada C., Ozaki T., Ando K., Suenaga Y., Inoue K., Ito Y., Okoshi R., Kageyama H., Kimura H., Miyazaki M., Nakagawara A.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 16693-16703
  • Peer Reviewed
• [Journal Article] NFBD1/MDC1 participates in the regulation of G2/M transition in mammalian cells. (2010)
  • Author(s): Bu Y., Suenaga Y., Okoshi R., Sang M., Kubo N., Song F., Nakagawara A., Ozaki T.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 397 Pages: 157-162
  • Peer Reviewed
• [Presentation] NCYMはMYCNを安定化し、神経芽腫悪性化に寄与する (2011)
  • Author(s): Yusuke Suenaga, Daisuke Matsumoto, Yoshiki Kaneko, Mohammad Shamim Hossain, Atushi Takatori, Yohko Nakamura, Miki Ohira, Seiki Haraguchi, Akira Nakagawara
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-03
• [Presentation] NCYMはMYCNを安定化し、神経芽腫悪性化に寄与する (2011)
  • Author(s): 末永雄介
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-03
• [Presentation] NCYMはMYCNを安定化し、神経芽腫悪性化に寄与する (2011)
  • Author(s): 末永雄介
  • Organizer: 第17回神経芽腫研究会・症例検討会
  • Place of Presentation: 京都府立医科大学
  • Year and Date: 2011-09-03
• [Presentation] NCYM stabilizes MYCN and contributes to aggresiveness of neuroblastoma (2011)
  • Author(s): Yusuke Suenaga, Daisuke Matsumoto, Yoshiki Kaneko, Mohammad Shamim Hossain, Atsushi Takatori, Yohko Nakamura, Miki Ohira, Seiki Haraguchi, Akira Nakagawara
  • Organizer: 5^<th> International p63/73 workshop
  • Place of Presentation: リヨン(フランス)
• [Presentation] NCYM stabilizes MYCN and contributes to aggresiveness of neuroblastoma (2011)
  • Author(s): 末永雄介
  • Organizer: 5^<th> International p63/73 workshop
  • Place of Presentation: フランス(リヨン)
• [Presentation] MYCNの相補鎖遺伝子産物NCYMは神経芽腫においてリプログラミングファクターの発現を誘導する (2010)
  • Author(s): 金子伊樹、末永雄介、松本大介、中村洋子、大平美紀、中川原章
  • Organizer: 第26回日本小児がん学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2010-12-18
• [Presentation] MYCNアンチセンス遺伝子NCYMは神経芽腫予後不良群で高発現し、MYCNの発現を誘導する (2010)
  • Author(s): 末永雄介、金子伊樹、松本大介、中村洋子、大平美紀、中川原章
  • Organizer: 第26回日本小児がん学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2010-12-17
• [Presentation] NCYM, an antisense transcript of MYCN, induces expression of reprogramming factors in human neuroblastoma (2010)
  • Author(s): Yoshiki Kaneko, Yusuke Suenage, Daisuke Matsumoto, Yohko Nakamura, Miki Ohira, Akira Nakagawara
  • Organizer: 第33回日本分子生物学会年会,第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2010-12-09
• [Presentation] NCYM, an antisense transcript of MYCN, induces expression of reprogramming factors in human neuroblastoma (2010)
  • Author(s): Yoshiki Kaneko, Yusuke Suenaga, Daisuke Matsumoto, Yohko Nakamura, Miki Ohira, Akira Nakagawara
  • Organizer: 第33回日本分子生物学会年会, 第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2010-12-09
• [Presentation] Natural MYCN antisense gene, NCYM, functions as an oncogene in neuroblastoma through induction of MYCN (2010)
  • Author(s): Yusuke Suenaga, Yoshiki Kaneko, Yoshiki Kaneko, Daisuke Matsumoto, Yohko Nakamura, Miki Ohira, Seiki Haraguchi, Akira Nakagawara
  • Organizer: 第69日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2010-09-23
• [Presentation] NCYM induces Oct3/4 expression in neuroblastoma (2010)
  • Author(s): Yoshiki kaneko, Yusuke Suenaga, Daisuke Matsumoto, Yohko Nakamura, Miki Ohira, Akira Nakagawara
  • Organizer: 第69日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2010-09-23
• [Presentation] Natural MYCN antisense gene, NCYM, functions asan oncogene in neuroblastoma through induction of MYCN (2010)
  • Author(s): Yusuke Suenaga, Yoshiki Kaneko, Daisuke Matsumoto, Yohko Nakamura, Miki Ohira, Seiki Haraguchi, Akira Nakagawara
  • Organizer: 第69日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2010-09-23
• [Presentation] NCYM, a protein product of an natural antisense MYCN gene co-amplified with MYCN, targets MYCN for functional modulation and affects the prognosis of neuroblastoma (2010)
  • Author(s): Yusuke Suenaga, Yoshiki Kaneko, Daisuke Matsumoto, Yohko Nakamura, Miki Ohira, Toshinori Ozaki, Akira Nakagawara
  • Organizer: Advances in Neuroblastoma Research 2010
  • Place of Presentation: The Stockholm City Conference Centre(Stockholm)
  • Year and Date: 2010-06-22
• [Presentation] NCYM induces Oct3/4 expression in neuroblastoma (2010)
  • Author(s): Yoshiki Kaneko, Yusuke Suenaga, Daisuke Matsumoto, Yohko Nakamura, Miki Ohira, Akira Nakagawara
  • Organizer: 日本分子生物学会第10回春季シンポジウム
  • Place of Presentation: ホテル松島大観荘(宮城郡)
  • Year and Date: 2010-06-07
• [Presentation] Transcriptional repression of MYCN is mediated by TAp63 in human neuroblastomas (2010)
  • Author(s): Yusuke Suenaga, Yoshiki Kaneko, Mami Yamamoto, Daisuke Matsumoto, Tomoe Ichikawa, Toshiyuki Moritan, Takehiko Kamijo, Yohko Nakamura, Miki Ohira, Toshinori Ozaki, Akira Nakagawara
  • Organizer: American Association for Cancer Research 101st ANNUAL MEETING
  • Place of Presentation: Washington Convention Center(Washington, DC)
  • Year and Date: 2010-04-21
• [Presentation] Transcriptional repression of MYCN is mediated byTAp63 in human neuroblastomas. (2010)
  • Author(s): Yusuke Suenaga, Yoshiki Kaneko, Mami Yamamoto, Daisuke Matsumoto, Tomoe Ichikawa, Toshiyuki Moritan, Takehiko Kamijo, Yohko Nakamura, Miki Ohira, Toshinori Ozaki, Akira Nakagawara
  • Organizer: American Association for Cancer Research 101st ANNUAL MEETING
  • Place of Presentation: Walter E.Washington Convention Center(Washington,DC)
  • Year and Date: 2010-04-21
• [Book] がん生物学イラストレイテッド (2011)
  • Author(s): 末永雄介
  • Total Pages: 5
  • Publisher: 羊土社
• [Book] がん生物学イラストレイテッド
  • Author(s): 末永雄介、中川原章
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.chiba-cc.jp/